哮喘联盟

   摘要
   研究目的：亚洲沙尘暴（ADS）包含大气尘粒，这些尘粒影响疾病控制情况，例如哮喘，但其导致哮喘急性加重的机制仍不清楚。本研究的目的是比较在ADS时收集的大气尘粒和从ADS发源地收集的（CJ-1 soil）尘粒对哮喘小鼠模型免疫佐剂效应及对气道炎症的影响。
   方法：在日本西部地区ADS时收集大气尘粒。NC/Nga小鼠经鼻内滴注ADS大气尘粒和/或尘螨（Df）共致敏，继续连用CJ-1 soil 和/或 Df 5天。Df致敏的小鼠在最后一次Df致敏后，继续经Df鼻内激发7天。激发结束后24小时，检测血清过敏原特异性抗体、支气管肺泡灌洗液（BALF）中的白细胞分类计数和炎症因子及气道炎症的组织病变。
   结果：经ADS大气尘粒和Df共同致敏的小鼠，其BALF的中性粒细胞和嗜酸性粒细胞计数上升，同时观察到肺部细支气管和血管周围气道炎症增强。经 ADS大气尘粒刺激的小鼠其Df特异性的血清IgE水平显著升高，但在CJ-1 soil刺激小鼠未出现这一变化。相比于CJ-1 soil刺激的小鼠，ADS大气尘粒处理的小鼠，其BALF中白介素-5、白介素-13、白介素-6、巨噬细胞、炎症蛋白-2的水平更高。
   结论：本研究的结果提示附着在ADS大气尘粒上的物质，而不是ADS尘粒本身，可能在免疫佐剂效应和气道炎症中发挥重要的作用。
 

（杨冬 审校）
PLoS One. 2014 Nov 11; 9(11): e111831.doi:10.1371/journal.pone.0111831.eCollection 2014.


 

Influence of asian dust particles on immune adjuvant effects and airway inflammation in asthma model mice.
 

Kurai J1, Watanabe M1, Tomita K2, Yamasaki HS3, Shimizu E1.
 

ABSTRACT
OBJECTIVE:An Asian dust storm (ADS) contains airborne particles that affect conditions such as asthma, but the mechanism of exacerbation is unclear. The objective of this study was to compare immune adjuvant effects and airway inflammation induced by airborne particles collected on ADS days and the original ADS soil (CJ-1 soil) in asthma model mice.
METHODS: Airborne particles were collected on ADS days in western Japan. NC/Nga mice were co-sensitized by intranasal instillation with ADS airborne particles and/or Dermatophagoides farinae (Df), and with CJ-1 soil and/or Df for 5 consecutive days. Df-sensitized mice were stimulated with Df challenge intranasally at 7 days after the last Df sensitization. At 24 hours after challenge, serum allergen specific antibody, differential leukocyte count and inflammatory cytokines in bronchoalveolar lavage fluid (BALF) were measured, and airway inflammation was examined histopathologically.
RESULTS:Co-sensitization with ADS airborne particles and Df increased the neutrophil and eosinophil counts in BALF. Augmentation of airway inflammation was also observed in peribronchiolar and perivascular lung areas. Df-specific serum IgE was significantly elevated by ADS airborne particles, but not by CJ-1 soil. Levels of interleukin (IL)-5, IL-13, IL-6, and macrophage inflammatory protein-2 were higher in BALF in mice treated with ADS airborne particles.
CONCLUSION: These results suggest that substances attached to ADS airborne particles that are not in the original ADS soil may play important roles in immune adjuvant effects and airway inflammation.
 

PLoS One. 2014 Nov 11; 9(11): e111831.doi:10.1371/journal.pone.0111831.eCollection 2014.