哮喘联盟

   摘要
   背景：本研究旨在评估可能是哮喘潜在诱因的白介素－9（IL-9）、IL-17、IL-22及IL-25的基因表达情况，以及炎症细胞因子的另一个来源——固有淋巴细胞（ILCs）的数量。
   目的：本研究旨在评估哮喘患者群体中新亚型辅助T淋巴细胞所分泌的细胞因子以及固有淋巴细胞的数量。
   方法：我们收集了23例中度至重度哮喘患者以及23例健康受试者的血样和痰标本。通过免疫印迹法检测哮喘患者过敏原的种类，通过定量的转录－聚合酶链反应检测相关细胞因子的基因表达水平，通过流式细胞术检测固有淋巴细胞的数量。
   结果：本项目的研究成果表明，哮喘患者群体IL-9、IL-17、IL-22以及 IL-25基因的表达量显著高于正常人群，在重度哮喘患者群体中尤其明显。而且以上细胞因子在血清样本中的增高程度高于痰标本。此外，哮喘患者群体中固有淋巴细胞的数量也显著高于正常人群。
   结论：本研究的结果显示，哮喘患者群体IL-9、IL-17、IL-22以及 IL-25基因的表达量增高，且增高的程度与其临床严重程度相关，因此，IL-9、IL-17、IL-22以及 IL-25可能在哮喘的发生发展过程中起着重要的作用。同时，本研究的结果还显示，作为炎症细胞因子的另一个来源，固有淋巴细胞的数量在哮喘患者中也显著增高，预示着该类型细胞可能与哮喘的病理发病机制相关。
 

（苏楠 审校）
Asia Pac Allergy. 2014 Oct;4(4):212-21. doi: 10.5415/apallergy.2014.4.4.212. Epub 2014 Oct 29.

 

Innate lymphoid cells and cytokines of the novel subtypes of helper T cells in asthma.
 

Sherkat R1, Yazdani R2, Ganjalikhani Hakemi M3, Homayouni V2, Farahani R2, Hosseini M4, Rezaei A3.
 

ABSTRACT
BACKGROUND: In this study, the expression of interleukin-9 (IL-9), IL-17, IL-22, and IL-25 genes that might be the potential predisposing factors for asthma as well as count of innate lymphoid cells (ILCs) as another source of inflammatory cytokines have been evaluated.
OBJECTIVE: The aim of this study was to evaluate the expression of newly identified helper T cells signature cytokines and amount of ILCs.
METHODS: Blood and sputum samples from 23 patients with moderate to severe asthma and 23 healthy volunteers were collected. The types of allergens to which our patients were sensitive were defined using immunoblotting method. Gene expression of studied cytokines was evaluated using quantitative transcription-polymerase chain reaction and ILCs were counted by the flow cytometry method.
RESULTS: In this research, the gene expressions of IL-9, IL-17, IL-22, and IL-25 were significantly higher in asthmatics, especially in the severe form of the disease. This increase was even higher in serum samples compared with sputum samples. Counting ILCs revealed their increase in comparison with normal people.
CONCLUSION: We showed the importance of IL-25, IL-22, IL-17, and IL-9 cytokines in patients with asthma as their expression levels are increased and these increase are correlated with the severity of the disease. We also showed that the increased amount of ILCs in asthmatics could confirm their potential role in the immunopathogenesis of asthma as another source of inflammatory cytokines.
 

Asia Pac Allergy. 2014 Oct;4(4):212-21. doi: 10.5415/apallergy.2014.4.4.212. Epub 2014 Oct 29.