来源于一种寄生虫的巨噬细胞迁移抑制因子能抑制过敏性哮喘患者外周血单核细胞Th2细胞因子的产生
2012/01/31
摘要
背景：我们前期研究显示，分离自寄生虫的人巨噬细胞迁移抑制因子(MIF)样蛋白(As-MIF)能通过募集CD4(+)CD25(+)Foxp3(+) T细胞抑制过敏性气道炎症。
目的：为了评价As-MIF作为哮喘治疗药物的临床重要性，我们研究了体外培养外周血单核细胞(PBMC)重组As-MIF(rAs-MIF)处理后的免疫反应。
方法：PBMC分离自10名过敏性哮喘患者、8名非过敏性哮喘患者和12名非过敏性健康受试者。将不同浓度的rAs-MIF加入到PBMC培养液中。3天后，通过ELISA检测Th2和Th1细胞因子。
结果：对于来自过敏性哮喘患者的PBMC，在rAs-MIF处理后，IL-4和IL-5的产生显著下降。这种抑制作用未在来自于非过敏性哮喘患者的PBMC中观察到。相反，对于来自不同人群的PBMC，rAs-MIF处理后IL-10的产生显著增加。
结论：本研究结果与前期小鼠研究类似，即As-MIF可以作为哮喘特异性治疗一种备选方法。
（林江涛 审校）
J Asthma. 2011 Dec 7. [Epub ahead of print]
Macrophage Migration Inhibitory Factor Isolated from a Parasite Inhibited Th2 Cytokine Production in PBMCs of Atopic Asthma Patients.
Paark HK, Cho MK, Park HY, Kim KU, Kim YS, Lee MK, Park SK, Kim DH, Yu HS.
Source
Department of Internal Medicine, School of Medicine, Pusan National University , Busan, Republic of Korea.
Abstract
BACKGROUND：In a previous study, we demonstrated that the human macrophage migration inhibitory factor (MIF)-like protein (As-MIF) isolated from helminths could inhibit allergic airway inflammation via the recruitment of CD4(+)CD25(+)Foxp3(+) T cells.
OBJECTIVE：To evaluate the clinical importance of As-MIF as an antiasthma drug, we evaluated immune responses after recombinant As-MIF (rAs-MIF) treatment in peripheral blood mononuclear cell (PBMC) cultures.
METHODS：PBMC was isolated from 10 patients with atopic asthma, 8 patients with nonatopic asthma, and 12 nonatopic healthy subjects, and various concentrations of rAs-MIF were transferred into the PBMC culture medium. After 3 days, we measured the levels of T helper 2 and T helper 1 cytokines via ELISA.
RESULTS：In atopic asthma, IL-4 and IL-5 production was significantly reduced in the PBMC cultures after rAs-MIF treatment. These inhibitory effects were not observed in the nonatopic asthma group. By way of contrast, IL-10 production in the PMBC cultures was significantly increased after rAs-MIF treatment in all experimental groups.
CONCLUSION：The results of this study are similar to those previously reported in a mouse study, suggesting that As-MIF might be a candidate for the specific treatment of asthma
J Asthma. 2011 Dec 7. [Epub ahead of print]
背景：我们前期研究显示，分离自寄生虫的人巨噬细胞迁移抑制因子(MIF)样蛋白(As-MIF)能通过募集CD4(+)CD25(+)Foxp3(+) T细胞抑制过敏性气道炎症。
目的：为了评价As-MIF作为哮喘治疗药物的临床重要性，我们研究了体外培养外周血单核细胞(PBMC)重组As-MIF(rAs-MIF)处理后的免疫反应。
方法：PBMC分离自10名过敏性哮喘患者、8名非过敏性哮喘患者和12名非过敏性健康受试者。将不同浓度的rAs-MIF加入到PBMC培养液中。3天后，通过ELISA检测Th2和Th1细胞因子。
结果：对于来自过敏性哮喘患者的PBMC，在rAs-MIF处理后，IL-4和IL-5的产生显著下降。这种抑制作用未在来自于非过敏性哮喘患者的PBMC中观察到。相反，对于来自不同人群的PBMC，rAs-MIF处理后IL-10的产生显著增加。
结论：本研究结果与前期小鼠研究类似，即As-MIF可以作为哮喘特异性治疗一种备选方法。
（林江涛 审校）
J Asthma. 2011 Dec 7. [Epub ahead of print]
Macrophage Migration Inhibitory Factor Isolated from a Parasite Inhibited Th2 Cytokine Production in PBMCs of Atopic Asthma Patients.
Paark HK, Cho MK, Park HY, Kim KU, Kim YS, Lee MK, Park SK, Kim DH, Yu HS.
Source
Department of Internal Medicine, School of Medicine, Pusan National University , Busan, Republic of Korea.
Abstract
BACKGROUND：In a previous study, we demonstrated that the human macrophage migration inhibitory factor (MIF)-like protein (As-MIF) isolated from helminths could inhibit allergic airway inflammation via the recruitment of CD4(+)CD25(+)Foxp3(+) T cells.
OBJECTIVE：To evaluate the clinical importance of As-MIF as an antiasthma drug, we evaluated immune responses after recombinant As-MIF (rAs-MIF) treatment in peripheral blood mononuclear cell (PBMC) cultures.
METHODS：PBMC was isolated from 10 patients with atopic asthma, 8 patients with nonatopic asthma, and 12 nonatopic healthy subjects, and various concentrations of rAs-MIF were transferred into the PBMC culture medium. After 3 days, we measured the levels of T helper 2 and T helper 1 cytokines via ELISA.
RESULTS：In atopic asthma, IL-4 and IL-5 production was significantly reduced in the PBMC cultures after rAs-MIF treatment. These inhibitory effects were not observed in the nonatopic asthma group. By way of contrast, IL-10 production in the PMBC cultures was significantly increased after rAs-MIF treatment in all experimental groups.
CONCLUSION：The results of this study are similar to those previously reported in a mouse study, suggesting that As-MIF might be a candidate for the specific treatment of asthma
J Asthma. 2011 Dec 7. [Epub ahead of print]
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小气道在成人哮喘患者临床表现中的重要性
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