超细颗粒和纳米颗粒诱导的氧化应激：血红素加氧酶-1和一氧化碳在抗炎途径中的作用
2012/01/31
摘要
前言：可吸入颗粒物（PM）被认为是与呼吸道疾病患病和死亡增加相关的一个环境因素。周围环境中的PM能促进易感人群炎症的发生，这是PM对呼吸系统的主要影响。PM增加由化学物质和金属成分诱导的氧化应激，成为其促炎效应的机制之一。细胞针对PM诱导的氧化应激的反应包括抗氧化防御的活化。
目的：本试验旨在研究血红素加氧酶-1（HO-1）和一氧化碳（CO）在PM诱导的氧化应激、炎症和毒性中抗炎和抗凋亡作用。
方法：基于时间过渡理论，采用Eyetech分析仪，通过激光技术检测微粒大小(Donner Technologies Ltd., Israel)。通过定量RT-PCR检测诱导痰液（IS）中HO-1含量。
结果：检测工作场所内外受暴露个体IS中的微小颗粒和纳米颗粒，可用于确定HO-1和CO的保护作用。
结论：正在进行的研究将在PM诱导的氧化应激模型中阐述HO-1和CO的保护作用。
（林江涛 审校）
J Asthma. 2011 Dec 22. [Epub ahead of print]
Ultrafine and Nanoparticles-Induced Oxidative Stress: The Role of Heme Oxygenase-1 and Carbon Monoxide as Anti-inflammatory Pathways.
Fireman E.
Source
Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center , Tel-Aviv, Israel.
Abstract
INTRODUCTION： Particulate matter (PM) is an environmental factor that has been associated with increased respiratory morbidity and mortality. The major effect of ambient PM on the pulmonary system is that it promotes inflammation in susceptible people. One of the mechanisms by which ambient PM exerts its proinflammatory effects is by generating increased oxidative stress induced by chemical compounds and metal constituents. Cellular responses to PM-induced oxidative stress include activation of antioxidant defense.
OBJECTIVE：To investigate the anti-inflammatory and anti-apoptotic roles of heme oxygenase-1 (HO-1) and carbon monoxide (CO) in the PM-induced oxidative stress arm, inflammation, and toxicity.
METHODS：Particle size analyses were performed according to a laser technique based on the time of transition theory using an Eyetech Analyzer (Donner Technologies Ltd., Israel). HO-1 was analyzed by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in induced sputum (IS) samples.
RESULTS：We demonstrate the feasibility of measuring micro- and nanoparticles in IS samples of exposed individuals in and out of the workplace to determine the protective role of HO-1 and CO.
CONCLUSIONS：Ongoing studies will further elucidate the protective role of HO-1 and CO in the PM-induced oxidative stress model.
J Asthma. 2011 Dec 22. [Epub ahead of print]
前言：可吸入颗粒物（PM）被认为是与呼吸道疾病患病和死亡增加相关的一个环境因素。周围环境中的PM能促进易感人群炎症的发生，这是PM对呼吸系统的主要影响。PM增加由化学物质和金属成分诱导的氧化应激，成为其促炎效应的机制之一。细胞针对PM诱导的氧化应激的反应包括抗氧化防御的活化。
目的：本试验旨在研究血红素加氧酶-1（HO-1）和一氧化碳（CO）在PM诱导的氧化应激、炎症和毒性中抗炎和抗凋亡作用。
方法：基于时间过渡理论，采用Eyetech分析仪，通过激光技术检测微粒大小(Donner Technologies Ltd., Israel)。通过定量RT-PCR检测诱导痰液（IS）中HO-1含量。
结果：检测工作场所内外受暴露个体IS中的微小颗粒和纳米颗粒，可用于确定HO-1和CO的保护作用。
结论：正在进行的研究将在PM诱导的氧化应激模型中阐述HO-1和CO的保护作用。
（林江涛 审校）
J Asthma. 2011 Dec 22. [Epub ahead of print]
Ultrafine and Nanoparticles-Induced Oxidative Stress: The Role of Heme Oxygenase-1 and Carbon Monoxide as Anti-inflammatory Pathways.
Fireman E.
Source
Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center , Tel-Aviv, Israel.
Abstract
INTRODUCTION： Particulate matter (PM) is an environmental factor that has been associated with increased respiratory morbidity and mortality. The major effect of ambient PM on the pulmonary system is that it promotes inflammation in susceptible people. One of the mechanisms by which ambient PM exerts its proinflammatory effects is by generating increased oxidative stress induced by chemical compounds and metal constituents. Cellular responses to PM-induced oxidative stress include activation of antioxidant defense.
OBJECTIVE：To investigate the anti-inflammatory and anti-apoptotic roles of heme oxygenase-1 (HO-1) and carbon monoxide (CO) in the PM-induced oxidative stress arm, inflammation, and toxicity.
METHODS：Particle size analyses were performed according to a laser technique based on the time of transition theory using an Eyetech Analyzer (Donner Technologies Ltd., Israel). HO-1 was analyzed by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in induced sputum (IS) samples.
RESULTS：We demonstrate the feasibility of measuring micro- and nanoparticles in IS samples of exposed individuals in and out of the workplace to determine the protective role of HO-1 and CO.
CONCLUSIONS：Ongoing studies will further elucidate the protective role of HO-1 and CO in the PM-induced oxidative stress model.
J Asthma. 2011 Dec 22. [Epub ahead of print]
上一篇：
来源于一种寄生虫的巨噬细胞迁移抑制因子能抑制过敏性哮喘患者外周血单核细胞Th2细胞因子的产生
下一篇：
哮喘小鼠的气道平滑肌肥厚与气道反应性增加相关
