匈牙利儿童期呼吸道感染中A型和B型呼吸道合胞病毒的检测和分子流行病学
2009/03/31
人呼吸道合胞病毒（hRSV）是全世界范围引起婴儿和儿童呼吸道感染的重要原因。目前在匈牙利hRSV的分子流行病学还不清楚。
目的：我们的研究目的是对匈牙利儿童期呼吸道感染中的hRSV进行分子水平的检测并进行遗传分析。
材料和方法：采集在Mosdós胸科医院儿科就诊的患有急性呼吸道感染的10岁以下儿童的鼻咽抽吸液。样本采集时间分别为2005～2006年和2006～2007年的10月15日～5月15日这段时间。前瞻性收集临床和流行病学数据。通过RT-PCR方法扩增表面融合糖蛋白（F）和附着糖蛋白（G）基因的病毒RNA。对PCR产物进行测序并进行系统进化分析。
结果：104名儿童的鼻咽抽吸液中23份样本（22.1%）检测出hRSV，其中男性16例（69.6%），女性7例（30.4%），第一季检出率为2%（1/49），第二季检出率为40%（22/55）。hRSV感染的发生时间为12月至3月。患儿平均年龄为2.1岁（1个月至8岁）。主要的症状有流鼻涕、发热、咳嗽和喘息。39.1%的感染hRSV的患儿有潜在疾病。根据F区，22例（96%）遗传学上病毒属于A型hRSV，1例（4%）为B型hRSV。根据B区，11例A型hRSV中8例（72.7%）为GA5组，3例（27.3%）为GA2组。几例患儿中病毒核苷酸序列相同。
结论：据我们所知，本研究匈牙利10岁以下儿童呼吸道感染中两种类型（A型和B型）的hRSV的分子检测和遗传分析的首次报道。冬春季hRSV是儿童呼吸道感染的重要原因，特别是婴儿，常需要住院治疗。
（刘国梁 审校）
Pankovics P, et al. Orv Hetil.2009 Jan 19;150(3):121-127.
Pankovics P, et al. Orv Hetil. 2009 Jan 19;150(3):121-7. Links
[Detection and molecular epidemiology of respiratory syncytial virus type A and B strains in childhood respiratory infections in Hungary.]
Human respiratory syncytial virus (hRSV) is one of the major causes of respiratory infection of infants and children worldwide. The molecular epidemiology of hRSV is unknown in Hungary. Aims: Our aims were the molecular detection and genetic analysis of hRSV from childhood respiratory infections in Hungary. Materials and methods: Nasopharyngeal aspirates were collected from children under the age of 10 years with acute respiratory infections provided by the Pediatric Department of the Hospital for Chest Diseases in Mosdós. Samples were taken from 15 October to 15 May in seasons of 2005/2006 and 2006/2007. The clinical and epidemiological data were collected prospectively. The amplification of the surface fusion glycoprotein (F) and the attachment glycoprotein (G) genes of viral RNA was made by RT-PCR method. PCR-products were sequenced and analyzed by phylogenetic analysis. Results: Nasopharyngeal aspirates of 104 children were examined out of which 23 (22.1%) samples - 16 males (69.6%) and 7 females (30.4%) - (first season: 1/49, 2%; second season: 22/55, 40%) contained hRSV. The hRSV infections were taking place from December to March. The average age was 2.1 years (1 month to 8 years). The leading symptoms were dropping nose, fever, cough and wheezing. Thirty-nine point one percent of the hRSV infected children had underlying disease. Based upon the F region 22 (96%), viruses genetically belonged to type A and 1 (4%) was classified as type B hRSV. Based upon the G region, out of the 11 type A viruses 8 (72.7%) belonged to group GA5 and 3 (27.3%) to group GA2. Viral nucleotide sequence was identical in several cases. Conclusions: To our knowledge, this is the first report on molecular detection and genetic analysis of the two types (A and B) of hRSV of children under the age of 10 with respiratory infections in Hungary. In winter and spring hRSV is an important cause of childhood respiratory infections, particularly in infants, often requiring hospitalization.
目的：我们的研究目的是对匈牙利儿童期呼吸道感染中的hRSV进行分子水平的检测并进行遗传分析。
材料和方法：采集在Mosdós胸科医院儿科就诊的患有急性呼吸道感染的10岁以下儿童的鼻咽抽吸液。样本采集时间分别为2005～2006年和2006～2007年的10月15日～5月15日这段时间。前瞻性收集临床和流行病学数据。通过RT-PCR方法扩增表面融合糖蛋白（F）和附着糖蛋白（G）基因的病毒RNA。对PCR产物进行测序并进行系统进化分析。
结果：104名儿童的鼻咽抽吸液中23份样本（22.1%）检测出hRSV，其中男性16例（69.6%），女性7例（30.4%），第一季检出率为2%（1/49），第二季检出率为40%（22/55）。hRSV感染的发生时间为12月至3月。患儿平均年龄为2.1岁（1个月至8岁）。主要的症状有流鼻涕、发热、咳嗽和喘息。39.1%的感染hRSV的患儿有潜在疾病。根据F区，22例（96%）遗传学上病毒属于A型hRSV，1例（4%）为B型hRSV。根据B区，11例A型hRSV中8例（72.7%）为GA5组，3例（27.3%）为GA2组。几例患儿中病毒核苷酸序列相同。
结论：据我们所知，本研究匈牙利10岁以下儿童呼吸道感染中两种类型（A型和B型）的hRSV的分子检测和遗传分析的首次报道。冬春季hRSV是儿童呼吸道感染的重要原因，特别是婴儿，常需要住院治疗。
（刘国梁 审校）
Pankovics P, et al. Orv Hetil.2009 Jan 19;150(3):121-127.
Pankovics P, et al. Orv Hetil. 2009 Jan 19;150(3):121-7. Links
[Detection and molecular epidemiology of respiratory syncytial virus type A and B strains in childhood respiratory infections in Hungary.]
Human respiratory syncytial virus (hRSV) is one of the major causes of respiratory infection of infants and children worldwide. The molecular epidemiology of hRSV is unknown in Hungary. Aims: Our aims were the molecular detection and genetic analysis of hRSV from childhood respiratory infections in Hungary. Materials and methods: Nasopharyngeal aspirates were collected from children under the age of 10 years with acute respiratory infections provided by the Pediatric Department of the Hospital for Chest Diseases in Mosdós. Samples were taken from 15 October to 15 May in seasons of 2005/2006 and 2006/2007. The clinical and epidemiological data were collected prospectively. The amplification of the surface fusion glycoprotein (F) and the attachment glycoprotein (G) genes of viral RNA was made by RT-PCR method. PCR-products were sequenced and analyzed by phylogenetic analysis. Results: Nasopharyngeal aspirates of 104 children were examined out of which 23 (22.1%) samples - 16 males (69.6%) and 7 females (30.4%) - (first season: 1/49, 2%; second season: 22/55, 40%) contained hRSV. The hRSV infections were taking place from December to March. The average age was 2.1 years (1 month to 8 years). The leading symptoms were dropping nose, fever, cough and wheezing. Thirty-nine point one percent of the hRSV infected children had underlying disease. Based upon the F region 22 (96%), viruses genetically belonged to type A and 1 (4%) was classified as type B hRSV. Based upon the G region, out of the 11 type A viruses 8 (72.7%) belonged to group GA5 and 3 (27.3%) to group GA2. Viral nucleotide sequence was identical in several cases. Conclusions: To our knowledge, this is the first report on molecular detection and genetic analysis of the two types (A and B) of hRSV of children under the age of 10 with respiratory infections in Hungary. In winter and spring hRSV is an important cause of childhood respiratory infections, particularly in infants, often requiring hospitalization.
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