母体血清而非母乳中的IL-5，IL-6和IL-13免疫标志物与婴幼抓挠相关
2016/07/27
摘要
背景：婴儿抓挠被认为与湿疹的早期发展有关。鲜为人知的是，母体的免疫标志物对婴儿抓挠的作用。本研究的目的是比较孕期母体血清免疫标志物（IMs）和母乳中免疫标志物对6月龄和12月龄婴儿抓挠的影响。
方法：在南卡罗来纳州的哥伦比亚和查尔斯顿招募怀孕的妇女，采集血（平均分娩前3周）和母乳（产后3周）标本。采用优化免疫法测定孕妇血清和乳清中干扰素(IFN)-γ，γ干扰素诱导蛋白10（IP-10）（或CXCL10）、CCL11、白细胞介素（IL）1β、IL-4、IL-5、IL-6、IL-8（CXCL8）、IL-10、IL-12(p70)、IL-13、转化生长因子(TGF)-β1和免疫球蛋白(Ig) A的浓度。在6个月和12个月的时候确定抓挠和皮肤的表现。用广义估计方程评估免疫标记物对反复抓挠的相对风险，考虑到自身内在相关性，并对混杂因素进行校正。
结果：178名妇女中，161人提供了血液样本，115人提供了母乳样本。由于出现了大比例的无法检测的数值，因此并未对孕妇血清和乳清中的IL-1β，IL-4、IL-10、IL-12、和CCL11进行分析。在孕妇血清中IL-6和IL-13水平最高的婴儿抓挠的风险更高（RR分别为 1.73和1.84；P≤ 0.002）；同样，血清IL-5水平高（与低的相比）的母亲所生的婴儿抓挠的风险也在增加（RR 1.60，P = 0.002）。母乳中的免疫标志物与抓挠无关。
结论：有抓挠并非医生诊断的湿疹与母体孕期更高水平的IL-5，IL-6和IL-13相关。有必要进行进一步的研究来确定母体血清标志物如何影响婴儿抓挠。
（苏欣 审校）
Allergy Asthma Clin Immunol. 2016 May 24;12:25. doi: 10.1186/s13223-016-0129-x. eCollection 2016.
Maternal serum but not breast milk IL-5, IL-6, and IL-13 immune markers are associated with scratching among infants.
Soto-Ramírez N1, Boyd K2, Zhang H1, Gangur V3, Goetzl L4, Karmaus W1.
Author information
Abstract
BACKGROUND:Scratching in infants is considered to be related to early development of eczema. Little is known about the effects of maternal immune markers on scratching among infants. The objective is to compare the risks related to maternal serum immune markers (IMs) during pregnancy and IMs in breast milk for the occurrence of scratching in infants at 6 and 12 months of age.
METHODS:Pregnant women were recruited in Columbia and Charleston, South Carolina. Blood (median 3 weeks prepartum) and breast milk (3 weeks postpartum) samples were collected. The concentrations of interferon (IFN)-γ, IFN gamma-induced protein 10 (IP-10) (or CXCL10), CCL11, interleukin (IL) 1β, IL-4, IL-5, IL-6, IL-8 (CXCL8), IL-10, IL-12 (p70), IL-13, transforming growth factor (TGF)-β1, and immunoglobulin (Ig) A in both maternal serum and whey were assayed using optimized immunoassays. Scratching and skin manifestations were ascertained at 6 and 12 months. Generalized estimating equations were used to estimate relative risks (RRs) of IMs for repeated measurements of scratching, considering intra-individual correlations and adjusting for confounders.
RESULTS:Of 178 women, 161 provided blood and 115 breast milk samples. IL-1β, IL-4, IL-10, IL-12, and CCL11 in maternal serum and whey were not analyzed due to a large proportion of non-detectable values. Infants in the highest tertile of IL-6 and IL-13 in maternal serum were at higher risk of scratching (RR 1.73 and 1.84, respectively; p ≤ 0.002) compared to infants in the first tertile; similarly, infants born to mothers with high (versus low) levels of serum IL-5 were also at increased risk (RR 1.60, p = 0.002). None of the breast milk IMs studied were associated with scratching.
CONCLUSIONS:Scratching but not doctors diagnosed eczema was associated with higher levels of maternal IL-5, IL-6, and IL-13 during pregnancy. Further investigations are necessary to determine how maternal serum IMs influence infants scratching.
KEYWORDS:Breast milk; IL-13; IL-5; IL-6; Immune markers; Maternal serum; Scratching episodes
Allergy Asthma Clin Immunol. 2016 May 24;12:25. doi: 10.1186/s13223-016-0129-x. eCollection 2016.
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