城市灰尘微生物：对过敏和喘息的影响
2016/07/27
摘要
简介：在城区，对探索室内灰尘微生物可能影响哮喘和过敏的发病机制的调查才刚刚开始。我们旨在探讨室内灰尘中早期真菌和细菌微生态与儿童后期过敏和喘息的关系。
方法:从LISAplus出生队列研究中的189个家庭收集灰尘样品，这些样本在小孩出生不久马上从客厅地板收集，并对真菌和细菌微生物进行测定。真菌和细菌的多样性用末端限制性片段长度多态性评估（tRFLP），并用辛普森多样性指数进行定义。喘息的结果和共同变量信息直到小孩10岁时会通过父母问卷调查获取。特应性致敏的信息，在6岁和10岁的时候获取。逻辑回归和广义估计方程（GEE）模型被用来研究微生物多样性和健康之间的关系。
结果：在校对过的分析中，逻辑回归分析显示，暴露于高真菌多样性的环境下，6岁时空气过敏原的致敏作用和10岁之前的喘息风险显著降低（校正的比值比(95%CI)分别为: 0.26 (0.10-0.70), 和0.42 (0.18-0.96)）。直到10岁时，纵向分析（GEE）显示这种相互关系减弱。高暴露于多样性细菌和测试的健康结果之间没有关联。
结论：早期高暴露于多样性真菌中可能有助于防止儿童早期的空气过敏原的致敏作用，但在儿童后期这种关联减弱的原因需要进一步的前瞻性研究。
（苏欣 审校）
Environ Health Perspect. 2016 May 27. [Epub ahead of print]
Urban Dust Microbiome: Impact on Later Atopy and Wheezing.
Tischer C1,2,3,4, Weikl F5, Probst AJ6, Standl M1, Heinrich J1,7, Pritsch K5.
Author information
Abstract
INTRODUCTION:Investigations in urban areas have just begun to explore how the indoor dust microbiome may affect the pathogenesis of asthmaand allery. We aimed to investigate the early fungal and bacterial microbiome in house dust with allergic sensitization and wheezing later in childhood.
METHODS:Individual dust samples from 189 homes of the LISAplus birth cohort study were collected shortly after birth from living room floors and profiled for fungal and bacterial microbiome. Fungal and bacterial diversity was assessed with terminal restriction fragment length polymorphism (tRFLP) and defined by the Simpson diversity index. Information on wheezing outcomes and co-variates until the age of 10 years was obtained by parental questionnaires. Information on specific allergic sensitization was available at 6 and 10 years. Logistic regression and General Estimation Equation (GEE) models were used to examine the relationship between microbial diversity and health outcomes.
RESULTS:Logistic regression analyses revealed a significantly reduced risk of developing sensitization to aero-allergens at 6 years and ever wheezing until the age of 10 years for exposure to higher fungal diversity (adjusted Odds Ratio aOR (95%CI): 0.26 (0.10-0.70)), and 0.42 (0.18-0.96), respectively), in adjusted analyses. The associations were attenuated for the longitudinal analyses (GEE) until the age of 10 years. There was no association between higher exposure to bacterial diversity and the tested health outcomes.
CONCLUSION:Higher early exposure to fungal diversity might help to prevent from developing sensitization to aero-allergens in early childhood, but the reasons for attenuated effects in later childhood require further prospective studies.
Environ Health Perspect. 2016 May 27. [Epub ahead of print]
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母体血清而非母乳中的IL-5，IL-6和IL-13免疫标志物与婴幼抓挠相关
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富含大豆油或鱼油的食物对过敏性气道炎症的保护作用
