哮喘联盟

   摘要
   背景：已有诸多研究探讨，维生素D受体(VDR)基因多态性作为独立因素或与维生素D水平联合与免疫相关疾病的进展有关。维生素D和维生素D受体与哮喘易感性独立相关。本研究旨在探讨VDR变异体是否独立或与维生素D水平共同与塞浦路斯青少年哮喘相关。
   方法：共纳入190例喘息患者，其中69例为哮喘活动期患者，另有671例为健康对照者。分别测定3种VDR基因型（BsmI、TaqI及ApaI）和血清25(OH)D水平。运用Logistic回归进行分层分析，根据维生素D缺乏(≤20 ng/ml)的情况来评价VDR变异体与哮喘的关系。
   结果：对照组和喘息患者、活动期哮喘患者的TaqI基因型分布显著不同，p值分别为 0.030和0.014。与对照组相比，tt基因型更多出现于喘息和哮喘患者中，三者比例分别为12.9%、19.2%、21.3%。而Bsml和ApaI基因多态性位点的分布在对照组、喘息患者和活动期哮喘患者之间无差异。根据维生素D缺乏情况进行分层，TaqI的tt基因型仅与维生素D水平正常(>20 ng/ml)的喘息 (OR: 1.97, 95% CI: 1.12-3.46)和哮喘(OR: 2.37, 95% CI: 1.02-5.52)显著相关，与维生素D缺乏的患者无关。
   结论：VDR的TaqI基因型与塞浦路斯青少年哮喘患者有关。该基因多态性可能主要促进维生素D水平正常(>20 ng/ml)患者的哮喘易感性。
 

（杨冬 审校）
Multidiscip Respir Med. 2015 Sep 4;10(1):26. doi: 10.1186/s40248-015-0025-0. eCollection 2015.

 

Association of vitamin D receptor gene polymorphisms and vitamin D levels with asthma and atopy in Cypriot adolescents: a case-control study.
 

Papadopoulou A1, Kouis P2, Middleton N3, Kolokotroni O4, Karpathios T5, Nicolaidou P5, Yiallouros PK6.
 

Abstract
BACKGROUND:Polymorphisms in the vitamin D receptor (VDR) gene have been studied in immune-related disorders either as independent contributors or in combination with vitamin D concentration. Vitamin D and VDR have been independently linked to asthma susceptibility. We investigated whether VDR variants were associated independently or in relation to vitamin D levels with asthma in Cypriot adolescents.
METHODS:We studied 190 current wheezers, 69 of which were categorized as active asthmatics and 671 healthy controls. We determined three VDR genotypes (BsmI, TaqI, ApaI) and measured serum 25(OH)D levels. Logistic regression and stratified analyses by the presence of hypovitaminosis D (≤20 ng/ml) were used to evaluate the association of the VDR variants with asthma.
RESULTS:The distribution of TaqI genotypes was significantly different between controls and current wheezers (p = 0.030) or active asthmatics (p = 0.014). The tt genotype was over-represented in wheezers (19.2 %) and asthmatics (21.3 %) compared to respective controls (12.9 %). No difference was observed between controls, current wheezers and active asthmatics in the genotypic distribution of BsmI and ApaI polymorphic sites. After stratification by the presence of hypovitaminosis D, a significant association was detected between tt genotype of TaqI polymorphism with wheezing (OR: 1.97, 95 % CI: 1.12, 3.46) and asthma (OR: 2.37, 95CI%: 1.02, 5.52) only in those with normal vitamin D levels (>20 ng/ml) but not in subjects with low vitamin D.
CONCLUSIONS:The minor TaqI genotype of VDR is associated with asthma in Cypriot adolescents. This polymorphism may contribute to asthma susceptibility primarily under conditions of normal vitamin D levels (>20 ng/ml).
 

Multidiscip Respir Med. 2015 Sep 4;10(1):26. doi: 10.1186/s40248-015-0025-0. eCollection 2015.