哮喘联盟

   摘要
   哮喘是一个常见的慢性炎症性气道疾病，已被公认为是一个重要的公共卫生问题。本研究通过卵清蛋白(OVA)诱导的过敏性哮喘小鼠动物模型和BEAS-2B细胞评估了褪黑素对过敏性哮喘的影响。，使用OVA致敏小鼠并进行气道激发诱导过敏性哮喘。初始OVA致敏后21-23天给予10和15mg/kg褪黑素，每日一次腹腔注射。研究褪黑素在肿瘤坏死因子 (TNF)-α刺激的BEAS-2B细胞中对前炎性细胞活素和基质金属蛋白酶-9(MMP-9)活性和表达的影响。褪黑素使用后可显著减少炎症细胞的数量、减轻气道高反应性以及降低免疫球蛋白(lg)E、白介素(IL)-4、IL-5和IL-13水平。褪黑素能减轻肺组织中的气道炎症和黏液产生，并显著抑制OVA激发诱导的MMP-9的高表达和活性。在TNF-α-刺激的BEAS-2B细胞中，褪黑素治疗显著降低促炎细胞因子水平，且具有较低的MMP-9表达和活性。这些结果表明褪黑素可有效抑制OVA激发诱导的过敏性哮喘。该结果提示褪黑素在哮喘治疗中的潜在作用。
 

（林江涛 审校）
Immunobiology.2014Aug10.pii:S0171-2985(14)00138-7.doi:10.1016/j.imbio.2014.08.004. [Epub ahead of print]

 

Melatonin reduces airway inflammation in ovalbumin-induced asthma.
 

Shin IS1, Park JW2, Shin NR3, Jeon CM3, Kwon OK3, Kim JS4, Kim JC5, Oh SR3, Ahn KS6.
 

ABSTRACT
Asthma is a common chronic inflammatory airway disease that is recognized as a major public health problem. In this study, we evaluated the effects of melatonin on allergic asthma using a murine model of ovalbumin (OVA)-induced allergic asthma and BEAS-2B cells. To induce allergic asthma, the mice were sensitized and airway-challenged with OVA. Melatonin was administered by intraperitoneal injection once per day at doses of 10 and 15mg/kg from days 21 to 23 after the initial OVA sensitization. We investigated the effects of melatonin on proinflammatory cytokines and matrix metalloproteinase-9 (MMP-9) activity and expression in tumor necrosis factor (TNF)-α-stimulated BEAS-2B cells. The administration of melatonin significantly decreased the number of inflammatory cells, airway hyperresponsiveness, and immunoglobulin (Ig) E with reductions in interleukin (IL)-4, IL-5, and IL-13. Melatonin attenuated the airway inflammation and the mucus production in lung tissue and significantly suppressed elevated MMP-9 expression and activity induced by an OVA challenge. In TNF-α-stimulated BEAS-2B cells, treatment with melatonin significantly reduced the levels of proinflammatory cytokines and lowered the expression and activity of MMP-9. These results indicate that melatonin effectively suppressed allergic asthma induced by an OVA challenge. The results suggest a potential role for melatonin in treating asthma.
 

Immunobiology.2014Aug10.pii:S0171-2985(14)00138-7.doi:10.1016/j.imbio.2014.08.004. [Epub ahead of print]