哮喘联盟

   摘要
   背景：局部IgE的产生可能是哮喘致病因素之一。本研究的目的是根据痰细胞表型评估痰液总IgE和细胞因子。
   方法：自CHU Liege 哮喘诊所纳入122例受试者，包括22例无过敏问题的健康人、41例嗜酸性细胞型（痰嗜酸性细胞≥3%）、16例中性粒细胞型（痰中性粒细胞>76%）和43例寡粒细胞型（痰嗜酸性细胞<3%且痰中性粒细胞<76%）哮喘患者。痰上清液总IgE(tIgE)以免疫CAP方法检测，细胞因子(IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-γ 和TNF-α)使用商品化Fluorokine MAP kits 以Luminex xMAP技术检测。
   结果：痰液样本浓缩后，大部分标本可检测出总IgE。哮喘患者痰液IgE 高于健康受试者。总体上，哮喘患者的细胞因子水平与健康人没有明显差异。嗜酸性细胞型哮喘患者 tIgE, IL-5 和IL-13较健康人(分别为P<0.001, P<0.001 和P<0.05)和寡粒细胞型哮喘患者(分别为P<0.01, P<0.001 和P<0.05)升高，同时IL-5较中性粒细胞型哮喘患者升高(P<0.01)。将患者按照痰液IgE含量分组，发现“高IgE组”(IgE ≥0.1 kU/l)痰上清液中IL-5, IL-6, IL-17和TNF-α浓度比“低IgE组”(IgE<0.1 kU/l)升高。
   结论：嗜酸性哮喘表型与痰IgE升高和Th2细胞因子表达相关。痰IgE升高与异质性细胞因子过多产生相关。
 

(刘国梁 审校)
PLoS One. 2013;8(3):e58388. doi: 10.1371/journal.pone.0058388. Epub 2013 Mar 26.
 

Sputum IgE and Cytokines in Asthma: Relationship with Sputum Cellular Profile.
 

Manise M, Holtappels G, Van Crombruggen K, Schleich F, Bachert C, Louis R.
 

Abstract
BACKGROUND: Local IgE production may play a role in asthma pathogenesis. The aim of the study was to assess sputum total IgE and cytokines in asthmatics according to sputum cellular phenotype.
METHODS: We studied 122 subjects including 22 non atopic healthy subjects, 41 eosinophilic (sputum eosinophils ≥3%), 16 neutrophilic (sputum neutrophils >76%) and 43 pauci-granulocytic asthmatics (sputum eosinophils  and sputum neutrophils ≤76%) recruited from the asthma clinic at CHU Liege. Sputum supernatant total IgE (tIgE) was measured by Immuno CAP and sputum supernatant cytokines (IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-γ and TNF-α) were measured with the Luminex xMAP Technology by using commercially available Fluorokine MAP kits.
RESULTS: After concentrating sputum samples, total IgE was detectable in the majority of subjects. Sputum IgE was raised in asthmatics when compared to healthy subjects. Overall, asthmatics did not significantly differ from healthy subjects with respect to cytokine levels. The eosinophilic asthma phenotype, however, was characterised by raised sputum tIgE, IL-5 and IL-13 compared to healthy subjects (p<0.001, p<0.001 and p<0.05 respectively) and pauci-granulocytic asthma (p<0.01, p<0.001 and p<0.05 respectively) and raised IL-5 compared to neutrophilic asthma (p<0.01). When patients were classified according to sputum IgE levels, it appeared that IL-5, IL-6, IL-17 and TNF-α sputum supernatant levels were raised in the "IgE high" asthmatics (IgE ≥0.1 kU/l) when compared to "IgE low" asthmatics (IgE<0.1 kU/l).
CONCLUSION: The eosinophilic asthma phenotype was associated with raised sputum IgE and a Th2 cytokine profile. Raised sputum IgE was associated with a heterogeneous cytokine overproduction.

PLoS One. 2013;8(3):e58388. doi: 10.1371/journal.pone.0058388. Epub 2013 Mar 26.