哮喘联盟

   摘要
   背景：在老年小鼠中，尚未深入研究年龄增加对低剂量抗原喂养诱导的口服耐受性的影响及其对抗原气道激发试验的影响。
   目的：本实验研究老年小鼠中是否能诱导口服耐受性，及其对过敏性气道炎症发展的影响。
   方法：致敏之前，年轻（6周）和老年（18个月）小鼠采用卵清蛋白(OVA)喂食以诱导抗原耐受性。最后一次抗原激发后检测血清抗原特异性免疫球蛋白（Igs）、支气管肺泡灌洗液（BALF）、肺组织学、CD4 + Foxp3+ Treg细胞计数、气道高反应性（AHR）。
   结果：老年小鼠致敏前喂食抗原能诱导口服耐受性，表现为抗原特异性IgE和IgG1下降，然而，该影响在年轻小鼠中更明显。在年轻小鼠中，口服耐受性的诱导与气道Treg细胞增加更为相关。尽管存在这些差异，口服耐受性能显著抑制老年小鼠的哮喘特征，包括BALF总细胞计数、嗜酸性粒细胞计数、细胞因子产生和AHR。
   结论：老年小鼠也能出现抗原口服耐受性，后者能抑制过敏性气道炎症的某些特征。

（刘国梁 审校）
J Asthma. 2013 Jan 9. [Epub ahead of print]

 

The Effect of Oral Tolerance on the Allergic Airway Response in Younger and Aged Mice.
 
Birmingham JM, Patil S, Li XM, Busse PJ.

Source
Division of Clinical Immunology, Department of Medicine, Mount Sinai School of Medicine , New York, NY , USA .

Abstract 
BACKGROUND：The effect of increased age on the induction of oral tolerance by low-dose antigen feeding and its effect on the response to antigen airway challenge in aged mice have not been well characterized.
OBJECTIVE：To determine whether oral tolerance can be induced in aged mice and its impact on the development of allergic airway inflammation.
METHODS：Younger (6 weeks old) and aged (18 months old) mice were fed ovalbumin (OVA) prior to sensitization to induce antigen tolerance. Serum antigen-specific immunoglobulins (Igs), bronchoalveolar lavage fluid (BALF), lung histology, enumeration of CD4 + Foxp3+ Treg cells, and airway hyperresponsiveness (AHR) were determined after the final antigen challenge.
RESULTS：Feeding antigen to aged mice prior to sensitization induced oral tolerance as determined by a decrease in antigen-specific IgE and IgG(1); however, the effect was greater in younger mice. Induction of oral tolerance was associated with a greater increase in airway Treg cells in the younger mice. Despite these differences, oral tolerance significantly suppressed features of asthma in aged mice, including BALF total cell and eosinophil numbers, cytokine production, and AHR.
CONCLUSIONS：Aged mice developed oral tolerance to antigen, which suppressed several features of allergic airway inflammation.

J Asthma. 2013 Jan 9. [Epub ahead of print]