严重哮喘患者气道平滑肌细胞趋化因子表达对糖皮质激素低敏感的机制研究
2013/02/08
糖皮质激素治疗严重哮喘患者疗效不佳。
研究严重哮喘患者气道平滑肌细胞是否对糖皮质激素低敏感。
选择严重哮喘（n=10）、非严重哮喘（n=10）患者及非哮喘对照者（n=12）支气管活检标本进行气道平滑肌细胞培养，比较糖皮质激素对三组气道平滑肌细胞TNF-α及 IFN-γ诱导的CCL11（嗜酸粒细胞活化趋化因子）、CXCL8（IL-8）及CX3CL1（趋化因子CX3）表达的抑制作用及糖皮质激素低敏感的机制。
Corticosteroid insensitivity of chemokine expression in airway smooth muscle of patients with severe asthma.
ChangPJ,BhavsarPK,MichaeloudesC,KhorasaniN,ChungKF.JAllergyClinImmunol.2012Oct;130(4):877-85.e5. doi: 10.1016/j.jaci.2012.07.017. Epub 2012 Sep 1.（附：影响因子：11.003）
Source
Airway Disease, National Heart and Lung Institute, Imperial College London, and the Biomedical Research Unit, Royal Brompton NHS Foundation Trust, London, United Kingdom.
Abstract
BACKGROUND:
Patients with severe asthma are less responsive to the beneficial effects of corticosteroid therapy.
OBJECTIVE:
We investigated whether corticosteroid insensitivity was present in airway smooth muscle cells (ASMCs) of patients with severe asthma.
METHODS:
ASMCs cultured from bronchial biopsy specimens of nonasthmatic control subjects (n = 12) and patients with nonsevere (n = 10) or severe (n = 10) asthma were compared for the effect of dexamethasone on suppression of TNF-α- and IFN-γ-induced CCL11 (eotaxin), CXCL8 (IL-8), and CX3CL1 (fractalkine) expression. The mechanisms of corticosteroid insensitivity are also determined.
RESULTS:
CCL11 release was higher in ASMCs of patients with nonsevere but not severe asthma and nonasthmatic control subjects; CXCL8 and CX3CL1 release were similar in all groups. In patients with severe asthma, dexamethasone caused less suppression of CCL11 and CXCL8 release induced by TNF-α. Dexamethasone potentiated TNF-α- and IFN-γ-induced CX3CL1 release equally in all 3 groups. TNF-α-induced phosphorylated p38 mitogen-activated protein kinase levels were increased in ASMCs from patients with severe asthma compared with those from patients with nonsevere asthma and nonasthmatic subjects, whereas TNF-α-induced phosphorylated c-Jun N-terminal kinase and phosphorylated extracellular signal-related kinase levels were increased in all asthmatic groups. A p38 inhibitor increased the inhibitory effect of dexamethasone.
ASMCs of patients with severe asthma are corticosteroid insensitive; this might be secondary to heightened p38 mitogen-activated protein kinase levels.
JAllergyClinImmunol.2012Oct;130(4):877-85.e5.doi:10.1016/j.jaci.2012.07.017. Epub 2012 Sep 1.
研究严重哮喘患者气道平滑肌细胞是否对糖皮质激素低敏感。
选择严重哮喘（n=10）、非严重哮喘（n=10）患者及非哮喘对照者（n=12）支气管活检标本进行气道平滑肌细胞培养，比较糖皮质激素对三组气道平滑肌细胞TNF-α及 IFN-γ诱导的CCL11（嗜酸粒细胞活化趋化因子）、CXCL8（IL-8）及CX3CL1（趋化因子CX3）表达的抑制作用及糖皮质激素低敏感的机制。
Corticosteroid insensitivity of chemokine expression in airway smooth muscle of patients with severe asthma.
ChangPJ,BhavsarPK,MichaeloudesC,KhorasaniN,ChungKF.JAllergyClinImmunol.2012Oct;130(4):877-85.e5. doi: 10.1016/j.jaci.2012.07.017. Epub 2012 Sep 1.（附：影响因子：11.003）
Source
Airway Disease, National Heart and Lung Institute, Imperial College London, and the Biomedical Research Unit, Royal Brompton NHS Foundation Trust, London, United Kingdom.
Abstract
BACKGROUND:
Patients with severe asthma are less responsive to the beneficial effects of corticosteroid therapy.
OBJECTIVE:
We investigated whether corticosteroid insensitivity was present in airway smooth muscle cells (ASMCs) of patients with severe asthma.
METHODS:
ASMCs cultured from bronchial biopsy specimens of nonasthmatic control subjects (n = 12) and patients with nonsevere (n = 10) or severe (n = 10) asthma were compared for the effect of dexamethasone on suppression of TNF-α- and IFN-γ-induced CCL11 (eotaxin), CXCL8 (IL-8), and CX3CL1 (fractalkine) expression. The mechanisms of corticosteroid insensitivity are also determined.
RESULTS:
CCL11 release was higher in ASMCs of patients with nonsevere but not severe asthma and nonasthmatic control subjects; CXCL8 and CX3CL1 release were similar in all groups. In patients with severe asthma, dexamethasone caused less suppression of CCL11 and CXCL8 release induced by TNF-α. Dexamethasone potentiated TNF-α- and IFN-γ-induced CX3CL1 release equally in all 3 groups. TNF-α-induced phosphorylated p38 mitogen-activated protein kinase levels were increased in ASMCs from patients with severe asthma compared with those from patients with nonsevere asthma and nonasthmatic subjects, whereas TNF-α-induced phosphorylated c-Jun N-terminal kinase and phosphorylated extracellular signal-related kinase levels were increased in all asthmatic groups. A p38 inhibitor increased the inhibitory effect of dexamethasone.
ASMCs of patients with severe asthma are corticosteroid insensitive; this might be secondary to heightened p38 mitogen-activated protein kinase levels.
JAllergyClinImmunol.2012Oct;130(4):877-85.e5.doi:10.1016/j.jaci.2012.07.017. Epub 2012 Sep 1.
（深圳市人民医院 王凌伟摘译 邱晨审校）
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