吸烟哮喘患者的痰液上清液中骨桥蛋白含量增加
2012/12/24
摘要
吸烟可调节哮喘患者气道的炎症形式。骨桥蛋白(OPN)与炎症和纤维化相关。哮喘患者痰液OPN水平增加,而且与哮喘严重程度、气道重构和炎症相关介质有关。为评价哮喘患者痰液上清液OPN水平、研究吸烟与炎症和气道重构相关介质和细胞的相关性,我们对103名哮喘患者(49名吸烟)和40名健康对照(20名吸烟)进行研究。受试者接受肺功能检测、支气管乙酰甲胆碱高反应性检测、诱导痰液细胞计数以及痰液上清液OPN、TGF-β1、IL-8、IL-13和ECP检测。所有介质采用酶免疫分析。与非吸烟的哮喘患者、非吸烟对照和吸烟对照者相比,吸烟哮喘患者OPN水平(pg/ml)显著增加(中位数分别为1120 (651,1817) vs. 197 (118,341) vs. 50 (42,70) vs. 102 (77,110) pg/ml;p<0.001)。回顾性分析显示,OPN与痰液中性粒细胞、IL-8和TGF-β1显著相关,其中与TGF-β1的相关性最强。这些相关性仅在吸烟的哮喘患者中观察到。吸烟能显著影响哮喘患者的OPN水平。吸烟哮喘患者中的OPN与痰液中性粒细胞、TGF-β1和IL-8的相关性表明,OPN在该表现型哮喘的中性粒细胞性炎症和气道重构中起到一定作用。
(刘国梁 审校)
Cytokine.2012 Oct 22. pii:S1043-4666(12)00719-3. doi: 10.1016/j.cyto.2012.10.002. [Epub ahead of print]
Increased levels of osteopontin in sputum supernatant of smoking asthmatics.
Hillas G, Loukides S, Kostikas K, Simoes D, Petta V, Konstantellou E, Emmanouil P, Papiris S, Koulouris N, Bakakos P.
Source
Department of Respiratory and Critical Care Medicine, Research Unit, "Sotiria" Chest Diseases Hospital, Athens, Greece. Electronic address: ghillas70@yahoo.gr.
Abstract
Smoking may modify the inflammatory pattern of the asthmatic airways. Osteopontin (OPN) has been associated with inflammation and fibrosis. In asthma, sputum levels of OPN are elevated and have been related to the underlying severity and to mediators expressing remodeling and inflammation. To evaluate the levels of OPN in sputum supernatants of asthmatic patients and to investigate the possible role of smoking as well as associations with mediators and cells involved in the inflammatory and remodeling process. We studied 103 asthma patients (49 smokers) and 40 healthy subjects (20 smokers) who underwent lung function tests, bronchial hyperresponsiveness to methacholine, and sputum induction for cell count identification and measurement of OPN, TGF-β1, IL-8, IL-13 and ECP in sputum supernatants. The concentrations of all mediators were measured using enzyme immunoassays. OPN levels (pg/ml) were significantly higher in smoking asthmatics compared to non-smoking asthmatics, and both non-smoking and smoking controls [median (interquartile ranges) 1120 (651,1817) vs. 197 (118,341) vs. 50 (42,70) vs. 102 (77,110)pg/ml, respectively; p<0.001]. Regression analysis provided significant associations between OPN and sputum neutrophils, IL-8 and TGF-β1, the most significant being the one with TGF-β1. These associations were present only in smoking asthmatics. Smoking habit significantly affects sputum OPN levels in asthma. The associations of OPN with sputum neutrophils, TGF-β1 and IL-8 in smoking asthmatics suggest a possible role for OPN in the neutrophilic inflammation and remodeling process in this phenotype of asthma.
Cytokine. 2012 Oct 22. pii: S1043-4666(12)00719-3. doi: 10.1016/j.cyto.2012.10.002. [Epub ahead of print]
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