哮喘患者的高血清精氨酸酶I水平：与高敏感性C反应蛋白的相关性
2010/12/31
摘要
背景：诸多研究已经对哮喘小鼠内诱导肺内精氨酸酶I进行了关注。然而，前期的研究对哮喘患者血清精氨酸酶活性的改变尚未达成一致意见。
目的：本试验旨在评价哮喘患者血清精氨酸酶I的临床相关性。
方法：对非吸烟哮喘患者（n = 23）和健康个体（n = 30），采用ELISA横断面检测血清精氨酸酶I，研究该酶水平与多项临床参数之间的相关性。
结果：与健康对照（27.2 ± 12.9 ng/mL）相比，哮喘患者血清精氨酸酶I 水平显著增加（67.4 ± 41.0 ng/mL）。在健康对照者，不同性别之间未见精氨酸酶I 水平存在差异，但不同年龄组之间存在显著差异。在哮喘患者，不同年龄、不同性别和不同激素吸入治疗患者之间未见血清精氨酸酶I 水平存在显著差异，但不同过敏状态患者之间存在显著差异。非过敏性哮喘患者血清精氨酸酶I 水平显著高于过敏性哮喘患者和健康对照，但过敏性哮喘患者与健康对照之间未见显著差异。Spearman相关分析显示，在健康对照组中，血清精氨酸酶I 水平与年龄呈现显著负相关，与红细胞计数呈正相关，而在哮喘患者中，血清精氨酸酶I 水平与丙氨酸转氨酶(ALT)、高敏感C反应蛋白(hs-CRP)呈现正相关，而与免疫球蛋白E(IgE)呈现负相关。
结论：在非过敏性哮喘患者中，较高的血清精氨酸酶I水平与气道炎症相关。
（刘国梁 审校）
J Asthma. 2010 Nov 1. [Epub ahead of print]
High Serum Arginase I Levels in Asthma: its Correlation with High-Sensitivity C-Reactive Protein.
Ogino K, Obase Y, Takahashi N, Shimizu H, Takigawa T, Wang DH, Ouchi K, Oka M.
Department of Public Health, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Abstract
Background. Much attention has been directed to the induction of arginase I in the lung of asthmatic mice. However, there is no agreement on the changes of serum arginase activity in asthmatic patients among previous studies. Objectives. The aim of this study was to evaluate the clinical relevance of serum arginase I in asthmatic patients.
Methods. Serum arginase I was examined cross-sectionally in non-smoking asthmatic patients (n = 23) and healthy individuals (n = 30) using enzyme-linked immunosorbent assay (ELISA) and its correlations with several clinical parameters were investigated. Results. Serum levels of arginase I were significantly increased in asthmatic patients (mean ± SD 67.4 ± 41.0 ng/mL) compared with healthy controls (27.2 ± 12.9 ng/mL). In healthy controls, a difference in arginase I levels was not observed between sex groups but was observed between age groups. In asthmatic patients, serum arginase I levels were not different between groups of age, sex, and inhalation steroid therapy but were different between groups of atopic status. Non-atopic asthmatic patients revealed significantly high serum arginase I levels compared with atopic asthmatic patients and healthy controls although no difference was observed between atopic asthmatic patients and healthy controls. Spearman’s correlation analysis showed that serum arginase I level had a significant negative correlation with age and a positive correlation with red blood cell numbers in healthy controls, whereas in asthmatic patients, it had significant positive correlations with alanine aminotransferase (ALT), high-sensitivity C-reactive protein (hs-CRP) and a negative correlation with immunoglobulin-E (IgE). Conclusions. High serum arginase I levels in asthmatic patients may be associated with airway inflammation in non-atopic asthma.
J Asthma. 2010 Nov 1. [Epub ahead of print]
背景：诸多研究已经对哮喘小鼠内诱导肺内精氨酸酶I进行了关注。然而，前期的研究对哮喘患者血清精氨酸酶活性的改变尚未达成一致意见。
目的：本试验旨在评价哮喘患者血清精氨酸酶I的临床相关性。
方法：对非吸烟哮喘患者（n = 23）和健康个体（n = 30），采用ELISA横断面检测血清精氨酸酶I，研究该酶水平与多项临床参数之间的相关性。
结果：与健康对照（27.2 ± 12.9 ng/mL）相比，哮喘患者血清精氨酸酶I 水平显著增加（67.4 ± 41.0 ng/mL）。在健康对照者，不同性别之间未见精氨酸酶I 水平存在差异，但不同年龄组之间存在显著差异。在哮喘患者，不同年龄、不同性别和不同激素吸入治疗患者之间未见血清精氨酸酶I 水平存在显著差异，但不同过敏状态患者之间存在显著差异。非过敏性哮喘患者血清精氨酸酶I 水平显著高于过敏性哮喘患者和健康对照，但过敏性哮喘患者与健康对照之间未见显著差异。Spearman相关分析显示，在健康对照组中，血清精氨酸酶I 水平与年龄呈现显著负相关，与红细胞计数呈正相关，而在哮喘患者中，血清精氨酸酶I 水平与丙氨酸转氨酶(ALT)、高敏感C反应蛋白(hs-CRP)呈现正相关，而与免疫球蛋白E(IgE)呈现负相关。
结论：在非过敏性哮喘患者中，较高的血清精氨酸酶I水平与气道炎症相关。
（刘国梁 审校）
J Asthma. 2010 Nov 1. [Epub ahead of print]
High Serum Arginase I Levels in Asthma: its Correlation with High-Sensitivity C-Reactive Protein.
Ogino K, Obase Y, Takahashi N, Shimizu H, Takigawa T, Wang DH, Ouchi K, Oka M.
Department of Public Health, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
Abstract
Background. Much attention has been directed to the induction of arginase I in the lung of asthmatic mice. However, there is no agreement on the changes of serum arginase activity in asthmatic patients among previous studies. Objectives. The aim of this study was to evaluate the clinical relevance of serum arginase I in asthmatic patients.
Methods. Serum arginase I was examined cross-sectionally in non-smoking asthmatic patients (n = 23) and healthy individuals (n = 30) using enzyme-linked immunosorbent assay (ELISA) and its correlations with several clinical parameters were investigated. Results. Serum levels of arginase I were significantly increased in asthmatic patients (mean ± SD 67.4 ± 41.0 ng/mL) compared with healthy controls (27.2 ± 12.9 ng/mL). In healthy controls, a difference in arginase I levels was not observed between sex groups but was observed between age groups. In asthmatic patients, serum arginase I levels were not different between groups of age, sex, and inhalation steroid therapy but were different between groups of atopic status. Non-atopic asthmatic patients revealed significantly high serum arginase I levels compared with atopic asthmatic patients and healthy controls although no difference was observed between atopic asthmatic patients and healthy controls. Spearman’s correlation analysis showed that serum arginase I level had a significant negative correlation with age and a positive correlation with red blood cell numbers in healthy controls, whereas in asthmatic patients, it had significant positive correlations with alanine aminotransferase (ALT), high-sensitivity C-reactive protein (hs-CRP) and a negative correlation with immunoglobulin-E (IgE). Conclusions. High serum arginase I levels in asthmatic patients may be associated with airway inflammation in non-atopic asthma.
J Asthma. 2010 Nov 1. [Epub ahead of print]
