运动增加哮喘患者呼出气冷凝液中的半胱胺酰白三烯浓度
2010/12/17
摘要
背景：诸多证据显示，半胱氨酰白三烯(Cys-LTs)在运动诱导的支气管收缩(EIB)中具有重要作用。然而，有关EIB中Cys-LTs 浓度的变化尚未进行过研究。
目的：本试验研究运动对哮喘患者气道中的Cys-LT浓度的影响，后者通过检测呼出气冷凝液(EBC) 中Cys-LT的浓度来表示。
方法：17名过敏性哮喘患者和6名健康受试者入选本项研究，哮喘患者存在EIB病史。运动前检测FEV1及运动后10分钟内检测两次FEV1。收集EBC，检测Cys-LT浓度。基线状态下检测呼出气一氧化氮水平，作为气道炎症的一个标志物。
结果：基线状态下，哮喘组患者Cys-LT 水平显著高于对照组（168 pg/mL /112–223/ vs. 77 pg/mL /36–119/, P=0.03）。所有哮喘患者在运动后均出现EBC Cys-LT浓度增加(n = 17, P=0.03)，该增加程度在出现运动后支气管痉挛的患者更为显著(n = 7, P =0.03)，而健康受试者未见EBC Cys-LT浓度变化(P =0.59)。哮喘患者诱导的FEV1下降与EBC Cys-LT浓度增加相关(r = −0.40, P=0.03)。
结论：哮喘患者运动后数分钟之内，EBC的Cys-LT浓度显著增加，提示运动后Cys-LT的释放参与了EIB。
（林江涛 审校）
J Asthma. 2010 Sep 27. [Epub ahead of print]
Exercise increases exhaled breath condensate cysteinyl leukotriene concentration in asthmatic patients.
Bikov AX, Gajd X00f3 Csi RX, Husz X00e1 R XC, Szili BX, L X00e1 Z X00e1 R ZX, Antus BX, Losonczy GX, Horvath I.
Department of Pulmonology, Semmelweis University, Budapest, Hungary.
Abstract
Background. Although the importance of cysteinyl leukotrienes (Cys-LTs) in exercise-induced bronchoconstriction (EIB) is supported by various sources of evidence, how the concentration of these mediators change during the development of EIB has not been investigated. Objectives. Our goal was to determine the effect of exercise on the concentration of airway Cys-LT in asthmatic patients by measuring Cys-LT in exhaled breath condensate (EBC). Methods. Seventeen atopic asthmatic patients with a previous history of EIB and six healthy volunteers were studied. Before and two times within 10 minutes after exercise challenge, FEV1 was measured and EBC was collected for Cys-LT measurement. Exhaled nitric oxide level, a marker of airway inflammation, was also determined at baseline. Results. Baseline Cys-LT level was higher in the asthmatic group versus healthy subjects (168 pg/mL /112–223/ vs. 77 pg/mL /36–119/, p = .03). EBC Cys-LT concentration increased in all asthmatic patients post-exercise (n = 17, p = .03), with the increase significantly greater in patients developing exercise-induced bronchospasm (n = 7, p = .03), whereas no change was observed in healthy controls (p = .59). The exercise-induced fall in FEV1 in asthmatics was related to the increase in EBC Cys-LT concentration (r = −0.40, p = .03). Conclusions. Our study shows that Cys-LT concentration of EBC is elevated minutes after physical exercise in asthmatic patients and strongly supports the concept that the release of this mediator is involved in the development of EIB.
J Asthma. 2010 Sep 27. [Epub ahead of print]
背景：诸多证据显示，半胱氨酰白三烯(Cys-LTs)在运动诱导的支气管收缩(EIB)中具有重要作用。然而，有关EIB中Cys-LTs 浓度的变化尚未进行过研究。
目的：本试验研究运动对哮喘患者气道中的Cys-LT浓度的影响，后者通过检测呼出气冷凝液(EBC) 中Cys-LT的浓度来表示。
方法：17名过敏性哮喘患者和6名健康受试者入选本项研究，哮喘患者存在EIB病史。运动前检测FEV1及运动后10分钟内检测两次FEV1。收集EBC，检测Cys-LT浓度。基线状态下检测呼出气一氧化氮水平，作为气道炎症的一个标志物。
结果：基线状态下，哮喘组患者Cys-LT 水平显著高于对照组（168 pg/mL /112–223/ vs. 77 pg/mL /36–119/, P=0.03）。所有哮喘患者在运动后均出现EBC Cys-LT浓度增加(n = 17, P=0.03)，该增加程度在出现运动后支气管痉挛的患者更为显著(n = 7, P =0.03)，而健康受试者未见EBC Cys-LT浓度变化(P =0.59)。哮喘患者诱导的FEV1下降与EBC Cys-LT浓度增加相关(r = −0.40, P=0.03)。
结论：哮喘患者运动后数分钟之内，EBC的Cys-LT浓度显著增加，提示运动后Cys-LT的释放参与了EIB。
（林江涛 审校）
J Asthma. 2010 Sep 27. [Epub ahead of print]
Exercise increases exhaled breath condensate cysteinyl leukotriene concentration in asthmatic patients.
Bikov AX, Gajd X00f3 Csi RX, Husz X00e1 R XC, Szili BX, L X00e1 Z X00e1 R ZX, Antus BX, Losonczy GX, Horvath I.
Department of Pulmonology, Semmelweis University, Budapest, Hungary.
Abstract
Background. Although the importance of cysteinyl leukotrienes (Cys-LTs) in exercise-induced bronchoconstriction (EIB) is supported by various sources of evidence, how the concentration of these mediators change during the development of EIB has not been investigated. Objectives. Our goal was to determine the effect of exercise on the concentration of airway Cys-LT in asthmatic patients by measuring Cys-LT in exhaled breath condensate (EBC). Methods. Seventeen atopic asthmatic patients with a previous history of EIB and six healthy volunteers were studied. Before and two times within 10 minutes after exercise challenge, FEV1 was measured and EBC was collected for Cys-LT measurement. Exhaled nitric oxide level, a marker of airway inflammation, was also determined at baseline. Results. Baseline Cys-LT level was higher in the asthmatic group versus healthy subjects (168 pg/mL /112–223/ vs. 77 pg/mL /36–119/, p = .03). EBC Cys-LT concentration increased in all asthmatic patients post-exercise (n = 17, p = .03), with the increase significantly greater in patients developing exercise-induced bronchospasm (n = 7, p = .03), whereas no change was observed in healthy controls (p = .59). The exercise-induced fall in FEV1 in asthmatics was related to the increase in EBC Cys-LT concentration (r = −0.40, p = .03). Conclusions. Our study shows that Cys-LT concentration of EBC is elevated minutes after physical exercise in asthmatic patients and strongly supports the concept that the release of this mediator is involved in the development of EIB.
J Asthma. 2010 Sep 27. [Epub ahead of print]
