生命早期应激对哮喘大鼠免疫功能的影响以及音乐治疗的作用
2010/09/14
摘要
目的：虽然研究已经显示，心理应激对支气管哮喘能产生不良影响，但对于生命早期的应激（出生后的社会心理环境）是否对成人哮喘产生长期影响，以及其潜在的病理生理机制，目前的客观证据较少。本试验旨在研究生命早期应激对成年哮喘大鼠免疫功能和下丘脑-垂体-肾上腺（HPA）轴反应的影响，并探索音乐治疗是否能缓解上述指标。
方法：40只雄性Wistar大鼠的幼鼠随机分为哮喘组、成年期应激的哮喘组、儿童期应激的哮喘组、音乐组和对照组。大鼠哮喘采用卵清蛋白诱导，采用束缚应激建立心理应激模型，莫扎特的奏鸣曲 K.448 用于音乐治疗模型。检测儿童期应激后和成人期OVA激发后血清皮质酮水平。最后一次OVA激发后检测血、肺和脑组织中的免疫指标。
结果：儿童期和成人期应激导致白细胞和嗜酸性粒细胞计数和血清白介素4水平增加。成人期应激组OVA激发后皮质酮水平增加，而儿童期应激组大鼠皮质酮在儿童期增加，但在成年期下降。白介素1β具有类似的趋势。音乐治疗组大鼠血清白介素4水平和皮质酮水平下降。
结论：儿童期和成年期应激导致在哮喘恶化中不同的HPA轴反应。我们结果首次发现音乐对哮喘大鼠的长期治疗作用。
（陈欣 审校）
J Asthma. 2010 Jun;47(5):526-531.
Effects of stress in early life on immune functions in rats with asthma and the effects of music therapy
Lu Y, Liu M, Shi S, Jiang H, Yang L, Liu X, Zhang Q, Pan F.
Institute of Medical Psychology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.
Abstract
OBJECTIVE: Although studies have shown that psychological stress has detrimental effects on bronchial asthma, there are few objective data on whether early-life stress, as early postnatal psychosocial environment, has a long-lasting effect on adult asthma and the potential pathophysiologic mechanism. This study aims to examine the effects on immune function and hypothalamic-pituitary-adrenal (HPA) axis responses in adult asthmatic rats that experienced stress in early life and the potential ameliorative effects of music therapy on these parameters.
METHODS: Forty male Wistar rat pups were randomly assigned to the asthma group, the adulthood-stressed asthma group, the childhood-stressed asthma group, the music group, and the control group. Restraint stress and Mozart’s Sonata K.448 were applied to ovalbumin (OVA)-induced asthmatic rats to establish psychological stress and music therapy models. The levels of serum corticosterone were examined in both childhood after stress and adulthood after OVA challenge. Immune indicators in blood, lung, and brain tissues were measured after the last OVA challenge.
RESULTS: Stress in both childhood and adulthood resulted in increases in leukocyte and eosinophil numbers and serum interleukin (IL)-4 levels. The adulthood-stressed group demonstrated increased corticosterone levels after challenge, whereas the childhood-stressed group showed increased corticosterone concentration in childhood but decreased level in adulthood. Central IL-1beta exhibited a similar tendency. Music group rats showed reduced serum IL-4 and corticosterone.
CONCLUSIONS: Stress in childhood and adulthood resulted in different HPA axis responsiveness in the exacerbation of markers of asthma. These data provide the first evidence of the long-term normalizing effects of music on asthmatic rats.
J Asthma. 2010 Jun;47(5):526-31.
目的：虽然研究已经显示，心理应激对支气管哮喘能产生不良影响，但对于生命早期的应激（出生后的社会心理环境）是否对成人哮喘产生长期影响，以及其潜在的病理生理机制，目前的客观证据较少。本试验旨在研究生命早期应激对成年哮喘大鼠免疫功能和下丘脑-垂体-肾上腺（HPA）轴反应的影响，并探索音乐治疗是否能缓解上述指标。
方法：40只雄性Wistar大鼠的幼鼠随机分为哮喘组、成年期应激的哮喘组、儿童期应激的哮喘组、音乐组和对照组。大鼠哮喘采用卵清蛋白诱导，采用束缚应激建立心理应激模型，莫扎特的奏鸣曲 K.448 用于音乐治疗模型。检测儿童期应激后和成人期OVA激发后血清皮质酮水平。最后一次OVA激发后检测血、肺和脑组织中的免疫指标。
结果：儿童期和成人期应激导致白细胞和嗜酸性粒细胞计数和血清白介素4水平增加。成人期应激组OVA激发后皮质酮水平增加，而儿童期应激组大鼠皮质酮在儿童期增加，但在成年期下降。白介素1β具有类似的趋势。音乐治疗组大鼠血清白介素4水平和皮质酮水平下降。
结论：儿童期和成年期应激导致在哮喘恶化中不同的HPA轴反应。我们结果首次发现音乐对哮喘大鼠的长期治疗作用。
（陈欣 审校）
J Asthma. 2010 Jun;47(5):526-531.
Effects of stress in early life on immune functions in rats with asthma and the effects of music therapy
Lu Y, Liu M, Shi S, Jiang H, Yang L, Liu X, Zhang Q, Pan F.
Institute of Medical Psychology, Shandong University School of Medicine, Jinan, Shandong, P. R. China.
Abstract
OBJECTIVE: Although studies have shown that psychological stress has detrimental effects on bronchial asthma, there are few objective data on whether early-life stress, as early postnatal psychosocial environment, has a long-lasting effect on adult asthma and the potential pathophysiologic mechanism. This study aims to examine the effects on immune function and hypothalamic-pituitary-adrenal (HPA) axis responses in adult asthmatic rats that experienced stress in early life and the potential ameliorative effects of music therapy on these parameters.
METHODS: Forty male Wistar rat pups were randomly assigned to the asthma group, the adulthood-stressed asthma group, the childhood-stressed asthma group, the music group, and the control group. Restraint stress and Mozart’s Sonata K.448 were applied to ovalbumin (OVA)-induced asthmatic rats to establish psychological stress and music therapy models. The levels of serum corticosterone were examined in both childhood after stress and adulthood after OVA challenge. Immune indicators in blood, lung, and brain tissues were measured after the last OVA challenge.
RESULTS: Stress in both childhood and adulthood resulted in increases in leukocyte and eosinophil numbers and serum interleukin (IL)-4 levels. The adulthood-stressed group demonstrated increased corticosterone levels after challenge, whereas the childhood-stressed group showed increased corticosterone concentration in childhood but decreased level in adulthood. Central IL-1beta exhibited a similar tendency. Music group rats showed reduced serum IL-4 and corticosterone.
CONCLUSIONS: Stress in childhood and adulthood resulted in different HPA axis responsiveness in the exacerbation of markers of asthma. These data provide the first evidence of the long-term normalizing effects of music on asthmatic rats.
J Asthma. 2010 Jun;47(5):526-31.
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年龄和性别对气道高反应性的影响
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学龄期患哮喘和过敏性鼻炎儿童的氧化状态变化
