台湾儿童中NOS1基因对哮喘和总IgE水平的影响及其与环境因素的相互作用
2010/09/14
摘要
哮喘是一个复杂的疾病，已知其受到遗传和环境因素的影响。本试验在台湾南部155名哮喘儿童和301名对照儿童中研究NOS1基因中内显子2的GT重复序列、内显子20的AAT重复序列以及外显子29的CA重复序列的微卫星多态性，以及NOS1基因与环境因素的相互作用。检测血清总IgE、进行过敏筛选试验和基因多态性监测。哮喘组患儿其NOS1基因的14/14-AAT 重复序列的基因型频率显著高于对照组(p = 0.01)。携带NOS1 14/14-AAT基因型的哮喘患儿，其总IgE含量高于其他多态性的哮喘患儿。在至少一个NOS1 14-AAT等位基因、蟑螂暴露、总血清IgE较高以及过敏原筛选试验阳性的哮喘患儿中，基因与环境的相互作用是对照儿童的3.83倍、6.86倍和8.04倍（所有校正后的p<0.001）。
本研究发现，伴14-AAT 重复序列的NOS1基因与儿童哮喘相关。环境因素和过敏状态将增加携带NOS1易感等位基因儿童的哮喘风险。
（苏楠 审校）
Pediatr Allergy Immunol. 2010 Jul 1. [Epub ahead of print]
The effects of NOS1 gene on asthma and total IgE levels in Taiwanese children, and the interactions with environmental factors
Wang TN, Tseng HI, Kao CC, Chu YT, Chen WY, Wu PF, Lee CH, Ko YC.
Department of Public Health, College of Health Science, Kaohsiung Medical University, Kaohsiung, Taiwan.
Abstract
Asthma is a complex disorder, which is known to be affected by interactions between genetic and environmental factors. The aim of this study was to investigate the three microsatellite polymorphisms of GT repeats in intron 2, AAT repeats in intron 20, and CA repeats in exon 29 of the NOS1 gene in 155 asthmatic children and 301 control children, and the interaction with environmental factors in southern Taiwan. Total serum IgE, phadiatop test and genetic polymorphisms were measured. The genotype frequency of 14/14-AAT repeats of the NOS1 gene was significantly higher in the asthmatic group (p = 0.01). Total IgE concentrations were higher in asthmatic children (p = 0.015) carrying the NOS1 14/14-AAT genotype than in subjects with other polymorphisms. The gene and environmental interaction effects were 3.83-fold, 6.86-fold, and 8.04-fold (all corrected p-values <0.001) between subjects carrying at least one NOS1 14-AAT allele and exposure to cockroaches, high levels of total IgE, and positive response against the phadiatop test in asthmatic children. The findings of this study provide strong evidence that NOS1 gene with 14-AAT tandem repeats has a significant effect in asthmatic children. Environmental factors and atopic status will enhance the asthmatic risk for children who carry NOS1 susceptible allele.
Pediatr Allergy Immunol. 2010 Jul 1. [Epub ahead of print]
哮喘是一个复杂的疾病，已知其受到遗传和环境因素的影响。本试验在台湾南部155名哮喘儿童和301名对照儿童中研究NOS1基因中内显子2的GT重复序列、内显子20的AAT重复序列以及外显子29的CA重复序列的微卫星多态性，以及NOS1基因与环境因素的相互作用。检测血清总IgE、进行过敏筛选试验和基因多态性监测。哮喘组患儿其NOS1基因的14/14-AAT 重复序列的基因型频率显著高于对照组(p = 0.01)。携带NOS1 14/14-AAT基因型的哮喘患儿，其总IgE含量高于其他多态性的哮喘患儿。在至少一个NOS1 14-AAT等位基因、蟑螂暴露、总血清IgE较高以及过敏原筛选试验阳性的哮喘患儿中，基因与环境的相互作用是对照儿童的3.83倍、6.86倍和8.04倍（所有校正后的p<0.001）。
本研究发现，伴14-AAT 重复序列的NOS1基因与儿童哮喘相关。环境因素和过敏状态将增加携带NOS1易感等位基因儿童的哮喘风险。
（苏楠 审校）
Pediatr Allergy Immunol. 2010 Jul 1. [Epub ahead of print]
The effects of NOS1 gene on asthma and total IgE levels in Taiwanese children, and the interactions with environmental factors
Wang TN, Tseng HI, Kao CC, Chu YT, Chen WY, Wu PF, Lee CH, Ko YC.
Department of Public Health, College of Health Science, Kaohsiung Medical University, Kaohsiung, Taiwan.
Abstract
Asthma is a complex disorder, which is known to be affected by interactions between genetic and environmental factors. The aim of this study was to investigate the three microsatellite polymorphisms of GT repeats in intron 2, AAT repeats in intron 20, and CA repeats in exon 29 of the NOS1 gene in 155 asthmatic children and 301 control children, and the interaction with environmental factors in southern Taiwan. Total serum IgE, phadiatop test and genetic polymorphisms were measured. The genotype frequency of 14/14-AAT repeats of the NOS1 gene was significantly higher in the asthmatic group (p = 0.01). Total IgE concentrations were higher in asthmatic children (p = 0.015) carrying the NOS1 14/14-AAT genotype than in subjects with other polymorphisms. The gene and environmental interaction effects were 3.83-fold, 6.86-fold, and 8.04-fold (all corrected p-values <0.001) between subjects carrying at least one NOS1 14-AAT allele and exposure to cockroaches, high levels of total IgE, and positive response against the phadiatop test in asthmatic children. The findings of this study provide strong evidence that NOS1 gene with 14-AAT tandem repeats has a significant effect in asthmatic children. Environmental factors and atopic status will enhance the asthmatic risk for children who carry NOS1 susceptible allele.
Pediatr Allergy Immunol. 2010 Jul 1. [Epub ahead of print]
上一篇：
学龄期患哮喘和过敏性鼻炎儿童的氧化状态变化
下一篇：
学龄前儿童家庭空调环境和交通环境暴露与哮喘和过敏性症状之间的关系
