小鼠哮喘模型中有氧训练逆转气道炎症和气道重构
2010/02/23
有氧训练（AT）能减少呼吸困难、运动诱发性支气管痉挛，同时改善需氧量和生活质量。然而对上述作用的机制了解甚少。
本实验旨在小鼠气道炎症和气道重构模型中评价AT对肺部慢性过敏性炎症的作用。
将小鼠暴露于生理盐水或卵白蛋白，并分为对照组、AT组、OVA组和OVA＋AT组。于第28天开始进行AT（每周5次，每次60分钟，连续4周）。对小鼠的呼吸力学、特异性IgE和IgG1、胶原及弹性纤维沉积、平滑肌厚度、上皮粘液、支气管周围嗜酸性粒细胞密度、CD3＋和CD4＋细胞、IL-4、IL-5、IL-13、IFNγ、IL-2、IL-1ra、IL-10、NF-κB及Foxp3进行评估。
OVA 组小鼠气道IgE和IgG1、嗜酸性粒细胞、CD3+及CD4+细胞、IL-4、IL-5、IL-13、NF-κB、胶原及弹性纤维均增加。与OVA 组相比，OVA+AT组小鼠气道IgE和IgG1增加，嗜酸性粒细胞、CD3+及CD4+细胞、IL-4、IL-5、IL-13、NF-κB、气道重构、粘液合成、平滑肌厚度及肺组织抗性和弹性均下降（P<0.05）。OVA +AT 组小鼠IL-10 和IL-1ra增加（P<0.05），该增加与Foxp3无关。
AT能逆转气道炎症和气道重构、Th2细胞反应，改善呼吸力学。上述结果可能与IL-10 和IL-1ra表达增加及NF-κB表达下降有关。
（刘国梁 审校）
Eur Respir J. 2009 Nov 6.
Aerobic training reverses airway inflammation and remodeling in asthma murine model.
Silva RA, Vieira RP, Duarte AC, Lopes FD, Perini A, Mauad T, Martins MA, Carvalho CR.
Aerobic training (AT) decreases dyspnoea, exercise-induced bronchospasm and improves aerobic capacity and quality of life, however the mechanisms for such benefits remains poorly understood. The aim of the present study was to evaluate the AT effects in a chronic model of allergic lung inflammation in mice after the establishment of airway inflammation and remodeling.Mice were divided in Control, AT, OVA, and OVA+AT groups exposed to saline or Ovalbumin. AT began on the 28(th) day(60 min/5x-wk/4-wks). Respiratory mechanics, specific IgE and IgG1, collagen and elastic fibers deposition, smooth muscle thickness, epithelial mucus, peribronchial density of eosinophils, CD3+ and CD4+,IL-4,IL-5,IL-13,IFN-gamma,IL-2,IL-1ra,IL-10, NF-kappaB, and Foxp3 were evaluated.OVA group presented increase of IgE and IgG1, eosinophils, CD3+,CD4+,IL-4,IL-5,IL-13,NF-kappaB, collagen and elastic, mucus synthesis, smooth muscle thickness and lung tissue resistance and elastance. OVA+AT presented increase of IgE and IgG1, and reduction of eosinophils, CD3+,CD4+,IL-4,IL-5,IL-13,NF-kappaB, airway remodeling, mucus synthesis, smooth muscle thickness and tissue resistance and elastance compared with OVA group (P<0.05). OVA+AT also shown increased of IL-10 and IL-1ra (P<0.05), independently of Foxp3.AT reversed airway inflammation and remodeling, Th2 response and improves respiratory mechanics. These results seem to occur by increase in the expression of IL-10 and IL-1ra and a decrease of NF-kappaB.
Silva RA, Vieira RP, Duarte AC, et al. Eur Respir J. 2009 Nov 6.
本实验旨在小鼠气道炎症和气道重构模型中评价AT对肺部慢性过敏性炎症的作用。
将小鼠暴露于生理盐水或卵白蛋白，并分为对照组、AT组、OVA组和OVA＋AT组。于第28天开始进行AT（每周5次，每次60分钟，连续4周）。对小鼠的呼吸力学、特异性IgE和IgG1、胶原及弹性纤维沉积、平滑肌厚度、上皮粘液、支气管周围嗜酸性粒细胞密度、CD3＋和CD4＋细胞、IL-4、IL-5、IL-13、IFNγ、IL-2、IL-1ra、IL-10、NF-κB及Foxp3进行评估。
OVA 组小鼠气道IgE和IgG1、嗜酸性粒细胞、CD3+及CD4+细胞、IL-4、IL-5、IL-13、NF-κB、胶原及弹性纤维均增加。与OVA 组相比，OVA+AT组小鼠气道IgE和IgG1增加，嗜酸性粒细胞、CD3+及CD4+细胞、IL-4、IL-5、IL-13、NF-κB、气道重构、粘液合成、平滑肌厚度及肺组织抗性和弹性均下降（P<0.05）。OVA +AT 组小鼠IL-10 和IL-1ra增加（P<0.05），该增加与Foxp3无关。
AT能逆转气道炎症和气道重构、Th2细胞反应，改善呼吸力学。上述结果可能与IL-10 和IL-1ra表达增加及NF-κB表达下降有关。
（刘国梁 审校）
Eur Respir J. 2009 Nov 6.
Aerobic training reverses airway inflammation and remodeling in asthma murine model.
Silva RA, Vieira RP, Duarte AC, Lopes FD, Perini A, Mauad T, Martins MA, Carvalho CR.
Aerobic training (AT) decreases dyspnoea, exercise-induced bronchospasm and improves aerobic capacity and quality of life, however the mechanisms for such benefits remains poorly understood. The aim of the present study was to evaluate the AT effects in a chronic model of allergic lung inflammation in mice after the establishment of airway inflammation and remodeling.Mice were divided in Control, AT, OVA, and OVA+AT groups exposed to saline or Ovalbumin. AT began on the 28(th) day(60 min/5x-wk/4-wks). Respiratory mechanics, specific IgE and IgG1, collagen and elastic fibers deposition, smooth muscle thickness, epithelial mucus, peribronchial density of eosinophils, CD3+ and CD4+,IL-4,IL-5,IL-13,IFN-gamma,IL-2,IL-1ra,IL-10, NF-kappaB, and Foxp3 were evaluated.OVA group presented increase of IgE and IgG1, eosinophils, CD3+,CD4+,IL-4,IL-5,IL-13,NF-kappaB, collagen and elastic, mucus synthesis, smooth muscle thickness and lung tissue resistance and elastance. OVA+AT presented increase of IgE and IgG1, and reduction of eosinophils, CD3+,CD4+,IL-4,IL-5,IL-13,NF-kappaB, airway remodeling, mucus synthesis, smooth muscle thickness and tissue resistance and elastance compared with OVA group (P<0.05). OVA+AT also shown increased of IL-10 and IL-1ra (P<0.05), independently of Foxp3.AT reversed airway inflammation and remodeling, Th2 response and improves respiratory mechanics. These results seem to occur by increase in the expression of IL-10 and IL-1ra and a decrease of NF-kappaB.
Silva RA, Vieira RP, Duarte AC, et al. Eur Respir J. 2009 Nov 6.
