嗜酸性粒细胞性支气管炎和哮喘患者支气管肺泡灌洗液中的8-异前列腺素含量
2009/11/17
背景：氧化应激在哮喘的病理生理学中起到重要作用。但尚未在非哮喘性嗜酸性粒细胞性支气管炎（EB）患者中评价气道的氧化应激。8-异前列腺素F2α是氧化应激的标志物。
目的：该研究旨在确定EB患者气道是否存在氧化应激（8-异前列腺素），以及8-异前列腺素是否与EB或哮喘患者的气道通气功能有关。
方法：对11名EB患者、10名哮喘患者和9名健康对照者的支气管肺泡灌洗液检测8-异前列腺素含量。8-异前列腺素的检测采用酶联免疫分析。
结果：结果显示，哮喘患者和EB患者支气管肺泡灌洗液中8-异前列腺素含量均增加。哮喘患者支气管肺泡灌洗液中8-异前列腺素含量中位数（12.78 pg/mL）显著高于EB患者（8.34 pg/mL）和健康对照者（5.07 pg/mL）。
结论：本研究显示，哮喘患者氧化应激显著增加，EB患者氧化应激严重程度轻于哮喘患者。由于该研究采用支气管肺泡灌洗液8-异前列腺素含量反映氧化应激，因此，哮喘患者和EB患者气道通气功能的差异可能与气道8-异前列腺素含量有关。
（苏楠 审校）
J Asthma. 2009 Sep;46(7):712-715.
BAL fluid 8-isoprostane concentrations in eosinophilic bronchitis and asthma.
Xie J, Zhang Q, Zhong N, Lai K.
BACKGROUND: Oxidative stress has an important role in the pathophysiology of asthma. But oxidative stress of airway has not been assessed in patients with nonasthmatic eosinophilic bronchitis (EB). 8-epi-prostaglandin F2alpha (8-isoprostane) is a biomarker of oxidative stress.
OBJECTIVES: We sought to determine whether oxidative stress (measured by 8-isoprostane) occurs in EB and whether 8-isoprostane is associated with airway function in EB and asthma.
METHODS: We measured 8-isoprostane concentrations in the bronchoalveolar lavage (BAL) fluid from 11 subjects with EB, 10 subjects with asthma, and 9 healthy control subjects. 8-isoprostane was measured by enzyme immunoassays.
RESULTS: We found that BAL fluid 8-isoprostane concentrations were raised both in EB and asthma. The median concentrations of 8-isoprostane in BAL fluid were significantly higher in subjects with asthma (12.78 pg/mL) when compared with EB (8.34 pg/mL) and healthy control subjects (5.07 pg/mL).
CONCLUSIONS: Our study shows that oxidative stress is increased significantly in asthmatic subjects and the degree of oxidative stress in EB subjects is milder than that in asthma, as reflected by 8-isoprostane concentrations in the BAL fluid. The difference in airway function observed in subjects with EB and asthma could be associated with different elevation in 8-isoprostane concentration in the airways.
J Asthma. 2009 Sep;46(7):712-5.
目的：该研究旨在确定EB患者气道是否存在氧化应激（8-异前列腺素），以及8-异前列腺素是否与EB或哮喘患者的气道通气功能有关。
方法：对11名EB患者、10名哮喘患者和9名健康对照者的支气管肺泡灌洗液检测8-异前列腺素含量。8-异前列腺素的检测采用酶联免疫分析。
结果：结果显示，哮喘患者和EB患者支气管肺泡灌洗液中8-异前列腺素含量均增加。哮喘患者支气管肺泡灌洗液中8-异前列腺素含量中位数（12.78 pg/mL）显著高于EB患者（8.34 pg/mL）和健康对照者（5.07 pg/mL）。
结论：本研究显示，哮喘患者氧化应激显著增加，EB患者氧化应激严重程度轻于哮喘患者。由于该研究采用支气管肺泡灌洗液8-异前列腺素含量反映氧化应激，因此，哮喘患者和EB患者气道通气功能的差异可能与气道8-异前列腺素含量有关。
（苏楠 审校）
J Asthma. 2009 Sep;46(7):712-715.
BAL fluid 8-isoprostane concentrations in eosinophilic bronchitis and asthma.
Xie J, Zhang Q, Zhong N, Lai K.
BACKGROUND: Oxidative stress has an important role in the pathophysiology of asthma. But oxidative stress of airway has not been assessed in patients with nonasthmatic eosinophilic bronchitis (EB). 8-epi-prostaglandin F2alpha (8-isoprostane) is a biomarker of oxidative stress.
OBJECTIVES: We sought to determine whether oxidative stress (measured by 8-isoprostane) occurs in EB and whether 8-isoprostane is associated with airway function in EB and asthma.
METHODS: We measured 8-isoprostane concentrations in the bronchoalveolar lavage (BAL) fluid from 11 subjects with EB, 10 subjects with asthma, and 9 healthy control subjects. 8-isoprostane was measured by enzyme immunoassays.
RESULTS: We found that BAL fluid 8-isoprostane concentrations were raised both in EB and asthma. The median concentrations of 8-isoprostane in BAL fluid were significantly higher in subjects with asthma (12.78 pg/mL) when compared with EB (8.34 pg/mL) and healthy control subjects (5.07 pg/mL).
CONCLUSIONS: Our study shows that oxidative stress is increased significantly in asthmatic subjects and the degree of oxidative stress in EB subjects is milder than that in asthma, as reflected by 8-isoprostane concentrations in the BAL fluid. The difference in airway function observed in subjects with EB and asthma could be associated with different elevation in 8-isoprostane concentration in the airways.
J Asthma. 2009 Sep;46(7):712-5.
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哮喘和COPD中炎症机制的差异
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代谢性酸中毒能改善哮喘患者的气道传导率
