IL15基因变异与哮喘和特应质不相关
2009/03/20
背景：白细胞介素-15（IL-15）能够促进CD8+细胞的激活和增殖并促进CD8+细胞向Th2细胞的转化。之前的一项研究描述了在单体型分析中IL15基因的5种多态性和哮喘相关。
目的：我们选择IL15在HapMap中的标签单核苷酸多态性（SNPs），系统研究在大规模人群样本中这些IL15相关性。
方法：应用MALDI-TOF MS 对3099名9～11岁的儿童进行横断面研究，分析7种IL15 SNPs的基因型。通过标准、有效的方法证实所有的儿童均为特应质表型。采用sas 9.1.3和haploview软件研究单个SNPs和单体型的作用。进行等效性检验以验证阴性结果的显著性。
结果：不论是单个IL15多态性还是单体型分析在多次检验校正后都未证实与哮喘或特异反应性相关。
结论：这些结果未证实之前病例对照研究的结果，这些结果表明IL15基因变型在哮喘或其他特异反应性疾病的发生中并未起到重要作用。
（林江涛 审校）
Pinto LA, et al. Allergy. 2008 Dec 24. [Epub ahead of print]
Pinto LA, et al. Allergy. 2008 Dec 24. [Epub ahead of print]
IL15 gene variants are not associated with asthma and atopy.
Background: Interleukin 15 (IL15) promotes activation and proliferation of CD8+ T cells and enhances the differentiation into Th2 cells. A previous study described five polymorphisms in the IL15 gene to be associated with asthma in a haplotype analysis.
Aim: We selected HapMap tagging single nucleotide polymorphisms (SNPs) from IL15 to systematically investigate these IL15 associations in a large population-based sample. Methods: Genotyping of seven IL15 SNPs was performed using MALDI-TOF MS in a cross-sectional study population of 3099 children from Dresden or Munich (age 9-11 years). All children were phenotyped by standardized and validated protocols for atopic phenotypes. Effects of single SNPs and haplotypes were studied using sas 9.1.3 and haploview. Equivalence tests were performed to prove the significance of negative results.
Results: Neither single IL15 polymorphisms nor haplotype analyses showed associations with asthma or atopy after correction for multiple testing. Conclusion: These results do not confirm previous case-control studies and suggest that IL15 gene variants do not play an important role in the development for asthma or other atopic disorders.
目的：我们选择IL15在HapMap中的标签单核苷酸多态性（SNPs），系统研究在大规模人群样本中这些IL15相关性。
方法：应用MALDI-TOF MS 对3099名9～11岁的儿童进行横断面研究，分析7种IL15 SNPs的基因型。通过标准、有效的方法证实所有的儿童均为特应质表型。采用sas 9.1.3和haploview软件研究单个SNPs和单体型的作用。进行等效性检验以验证阴性结果的显著性。
结果：不论是单个IL15多态性还是单体型分析在多次检验校正后都未证实与哮喘或特异反应性相关。
结论：这些结果未证实之前病例对照研究的结果，这些结果表明IL15基因变型在哮喘或其他特异反应性疾病的发生中并未起到重要作用。
（林江涛 审校）
Pinto LA, et al. Allergy. 2008 Dec 24. [Epub ahead of print]
Pinto LA, et al. Allergy. 2008 Dec 24. [Epub ahead of print]
IL15 gene variants are not associated with asthma and atopy.
Background: Interleukin 15 (IL15) promotes activation and proliferation of CD8+ T cells and enhances the differentiation into Th2 cells. A previous study described five polymorphisms in the IL15 gene to be associated with asthma in a haplotype analysis.
Aim: We selected HapMap tagging single nucleotide polymorphisms (SNPs) from IL15 to systematically investigate these IL15 associations in a large population-based sample. Methods: Genotyping of seven IL15 SNPs was performed using MALDI-TOF MS in a cross-sectional study population of 3099 children from Dresden or Munich (age 9-11 years). All children were phenotyped by standardized and validated protocols for atopic phenotypes. Effects of single SNPs and haplotypes were studied using sas 9.1.3 and haploview. Equivalence tests were performed to prove the significance of negative results.
Results: Neither single IL15 polymorphisms nor haplotype analyses showed associations with asthma or atopy after correction for multiple testing. Conclusion: These results do not confirm previous case-control studies and suggest that IL15 gene variants do not play an important role in the development for asthma or other atopic disorders.
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