血液循环中MicroRNAs和T细胞因子的表达与哮喘发作的特点有关
2020/01/07
摘要
目的：哮喘恶化的免疫学机制尚未阐明。本研究的目的是评估不同哮喘发作特征与选择性表达的血清microRNA（miRNA）和T细胞亚群的关系。
方法：对21例哮喘患者在哮喘发作期间（急性发作访视[EV]和急性发作6周后随访[FV]）进行研究。在两次访视中，进行肺功能测定，呼出一氧化氮（FeNO）测定，并收集鼻咽和血液样品。鼻咽标本用多重聚合酶链反应（PCR）检测呼吸道病毒，并进行细菌培养。实时PCR检测血清miRNAs。流式细胞术检测T细胞表面标志物、嗜酸性粒细胞祖细胞和细胞内细胞因子。
结果：2/3的患者有中度或重度哮喘发作，FV在哮喘控制方面有明显改善。血清miRNA-126a、miRNA-16和miRNA-21的平均表达在EV组显著低于FV组。在EV时，miRNA-29b与FeNO相关（r=0.44，P<0.05），7个miRNA检测中有5个与肺功能检测相关。EV组的CD 45+CD4+IL-4+细胞明显高于FV组，T调节细胞和嗜酸性粒细胞前体细胞与哮喘控制呈正相关。在EV时，血清miRNAs与表达IL-4、IL-17、IL-22和干扰素γ的T细胞数呈负相关，而在FV时，与T细胞亚群呈正相关和负相关。鼻咽液中检测到的病原体（病毒和细菌）与临床、功能和免疫指标均无相关性。
结论：哮喘发作期表观遗传失调可能与呼吸功能、气道炎症及T细胞因子表达有关。
Circulating MicroRNAs and T-Cell Cytokine Expression Are Associated With the Characteristics of Asthma Exacerbation.
Wardzyńska A, Pawełczyk M, Rywaniak J, Kurowski M, Makowska JS, Kowalski ML.
Abstract
PURPOSE: Immunological mechanisms underlying asthma exacerbation have not been elucidated. The aim of this study was to assess the associations of various asthma exacerbation traits with selected serum microRNA (miRNA) expression and T-cell subpopulations.
METHODS: Twenty-one asthmatics were studied during asthma exacerbation (exacerbation visit [EV] and the follow-up visit [FV] at 6 weeks). At both visits, spirometry was performed, fractional exhaled nitric oxide (FeNO) was measured, and nasopharyngeal and blood samples were collected. In nasopharyngeal samples, respiratory viruses were assayed by multiplex polymerase chain reaction (PCR), and bacterial cultures were performed. Serum miRNAs were assayed with real-time PCR. T-cell surface markers, eosinophil progenitors and intracellular cytokines were assessed by flow cytometry.
RESULTS: Two-thirds of patients had moderate or severe exacerbation and the FV, overall improvement in asthma control was observed. The mean expression of serum miRNA-126a, miRNA-16 and miRNA-21 was significantly lower at the EV than at the FV. At EV, miRNA-29b correlated with FeNO (r = 0.44, P < 0.05), and 5 of 7 miRNA tested correlated with pulmonary function tests. The number of cluster of differentiation (CD)45+CD4+interleukin (IL)4+ cells was significantly higher at the EV than at the FV, and positive correlations of T-regulatory cells and eosinophil progenitors with asthma control was found. At the EV, serum miRNAs negatively correlated with the number of T cells expressing IL-4, IL-17, IL-22 and interferon gamma, while at the FV both positive and negative correlations with T-cell subsets were observed. No association of detected pathogen (viruses and bacteria) in nasopharyngeal fluid with clinical, functional and immunological parameters was found.
CONCLUSIONS: Epigenetic dysregulation during asthma exacerbation could be related to respiratory function, airway inflammation and T-cell cytokine expression.
目的：哮喘恶化的免疫学机制尚未阐明。本研究的目的是评估不同哮喘发作特征与选择性表达的血清microRNA（miRNA）和T细胞亚群的关系。
方法：对21例哮喘患者在哮喘发作期间（急性发作访视[EV]和急性发作6周后随访[FV]）进行研究。在两次访视中，进行肺功能测定，呼出一氧化氮（FeNO）测定，并收集鼻咽和血液样品。鼻咽标本用多重聚合酶链反应（PCR）检测呼吸道病毒，并进行细菌培养。实时PCR检测血清miRNAs。流式细胞术检测T细胞表面标志物、嗜酸性粒细胞祖细胞和细胞内细胞因子。
结果：2/3的患者有中度或重度哮喘发作，FV在哮喘控制方面有明显改善。血清miRNA-126a、miRNA-16和miRNA-21的平均表达在EV组显著低于FV组。在EV时，miRNA-29b与FeNO相关（r=0.44，P<0.05），7个miRNA检测中有5个与肺功能检测相关。EV组的CD 45+CD4+IL-4+细胞明显高于FV组，T调节细胞和嗜酸性粒细胞前体细胞与哮喘控制呈正相关。在EV时，血清miRNAs与表达IL-4、IL-17、IL-22和干扰素γ的T细胞数呈负相关，而在FV时，与T细胞亚群呈正相关和负相关。鼻咽液中检测到的病原体（病毒和细菌）与临床、功能和免疫指标均无相关性。
结论：哮喘发作期表观遗传失调可能与呼吸功能、气道炎症及T细胞因子表达有关。
(中日友好医院呼吸与危重症医学科 李红雯 摘译 林江涛 审校)
(Allergy Asthma Immunol Res. 2020 Jan;12(1):125-136. doi: 10.4168/aair.2020.12.1.125.)
(Allergy Asthma Immunol Res. 2020 Jan;12(1):125-136. doi: 10.4168/aair.2020.12.1.125.)
Circulating MicroRNAs and T-Cell Cytokine Expression Are Associated With the Characteristics of Asthma Exacerbation.
Wardzyńska A, Pawełczyk M, Rywaniak J, Kurowski M, Makowska JS, Kowalski ML.
Abstract
PURPOSE: Immunological mechanisms underlying asthma exacerbation have not been elucidated. The aim of this study was to assess the associations of various asthma exacerbation traits with selected serum microRNA (miRNA) expression and T-cell subpopulations.
METHODS: Twenty-one asthmatics were studied during asthma exacerbation (exacerbation visit [EV] and the follow-up visit [FV] at 6 weeks). At both visits, spirometry was performed, fractional exhaled nitric oxide (FeNO) was measured, and nasopharyngeal and blood samples were collected. In nasopharyngeal samples, respiratory viruses were assayed by multiplex polymerase chain reaction (PCR), and bacterial cultures were performed. Serum miRNAs were assayed with real-time PCR. T-cell surface markers, eosinophil progenitors and intracellular cytokines were assessed by flow cytometry.
RESULTS: Two-thirds of patients had moderate or severe exacerbation and the FV, overall improvement in asthma control was observed. The mean expression of serum miRNA-126a, miRNA-16 and miRNA-21 was significantly lower at the EV than at the FV. At EV, miRNA-29b correlated with FeNO (r = 0.44, P < 0.05), and 5 of 7 miRNA tested correlated with pulmonary function tests. The number of cluster of differentiation (CD)45+CD4+interleukin (IL)4+ cells was significantly higher at the EV than at the FV, and positive correlations of T-regulatory cells and eosinophil progenitors with asthma control was found. At the EV, serum miRNAs negatively correlated with the number of T cells expressing IL-4, IL-17, IL-22 and interferon gamma, while at the FV both positive and negative correlations with T-cell subsets were observed. No association of detected pathogen (viruses and bacteria) in nasopharyngeal fluid with clinical, functional and immunological parameters was found.
CONCLUSIONS: Epigenetic dysregulation during asthma exacerbation could be related to respiratory function, airway inflammation and T-cell cytokine expression.
上一篇：
鼻病毒激发后生物标记物波动动态显示哮喘患者适应能力丧失
下一篇：
白细胞介素2调节人嗜碱性细胞的活化
