室内细菌微生物群与10.5岁前哮喘的发展
2019/09/09
摘要
背景：早期室内细菌暴露与哮喘风险相关，但特定细菌属的作用却知之甚少。
目的：确定室内微生物群中的个别细菌属是否能预测哮喘的发展。
方法：收集2个月大时起居室的灰尘样本。尘埃微生物群的特征为细菌16S核糖体RNA基因的测序扩增子。对儿童（N = 373）进行随访，直至年龄为10.5岁。
结果：细菌群丰度与哮喘相关性经调整后呈负相关（p = 0.03）。哮喘患者家中的系统微生物群组成与非哮喘患者家庭的特征不同（加权UniFrac，调整后的关联，PERMANOVA-S，p = 0.02）。加权UniFrac距离矩阵主坐标分析的前两轴评分与哮喘呈负相关。在尘埃样本中检测到的658个属中，41个基因的相对丰度与其中的一个轴相关（r> | 0.4 |）。乳球菌属是哮喘的危险因素（aOR 1.36,95％CI 1.13-1.63 / IQR变化）。 12个细菌属（主要来自放线菌目）的丰度与较低的哮喘风险相关（p <0.10），但并不相互独立。这12个相互关联的属的总相对丰度具有显著的保护作用，也一大部分解释了丰富性与较少哮喘发生的关联。
结论：我们的数据证实，婴儿家庭微生物群的系统差异与随后的哮喘风险相关，并且表明与个别细菌分类群或仅仅是丰富度相比，所选取的细菌群落与哮喘保护的关联性更高。
Indoor Bacterial Microbiota and the Development of Asthma by 10.5 years of age.
Karvonen AM, Kirjavainen PV, Täubel M, Jayaprakash B, Adams RI, Sordillo JE, Gold DR, Hyvärinen A, Remes S, von Mutius E, Pekkanen J.
Abstract
BACKGROUND:Early-life indoor bacterial exposure is associated with the risk of asthma but the roles of specific bacterial genera are poorly understood.
OBJECTIVE:To determine whether individual bacterial genera in indoor microbiota predict the development of asthma.
METHODS:Dust samples from living rooms were collected at 2 months of age. The dust microbiota was characterized by Illumina MiSeq sequencing amplicons of bacterial 16S ribosomal RNA gene. Children (N=373) were followed up for ever asthma until the age of 10.5 years.
RESULTS:Richness was inversely associated with asthma after adjustments (p=0.03). The phylogenetic microbiota composition in asthmatics' homes was characteristically different from non-asthmatics' homes (weighted UniFrac, adjusted association, PERMANOVA-S, p=0.02). The first two axis scores of principal coordinate analysis of the weighted UniFrac-distance matrix were inversely associated with asthma. Out of 658 genera detected in the dust samples, the relative abundances of 41genera correlated (r>|0.4|) with one of these axes. Lactococcus genus was a risk factor for asthma (aOR 1.36, 95%CI 1.13-1.63 per IQR change). The abundance of twelve bacterial genera (mostly from Actinomycetales order) was associated with lower asthma risk (p<0.10), though not independently of each other. The sum relative abundance of these12 intercorrelated genera was significantly protective and explained majority of the association of richness with less asthma.
CONCLUSIONS:Our data confirms that phylogenetic differences in infant home microbiota are associated with subsequent asthma risk and suggest that communities of selected bacteria are more strongly linked to asthma protection than individual bacterial taxon or mere richness.
背景：早期室内细菌暴露与哮喘风险相关，但特定细菌属的作用却知之甚少。
目的：确定室内微生物群中的个别细菌属是否能预测哮喘的发展。
方法：收集2个月大时起居室的灰尘样本。尘埃微生物群的特征为细菌16S核糖体RNA基因的测序扩增子。对儿童（N = 373）进行随访，直至年龄为10.5岁。
结果：细菌群丰度与哮喘相关性经调整后呈负相关（p = 0.03）。哮喘患者家中的系统微生物群组成与非哮喘患者家庭的特征不同（加权UniFrac，调整后的关联，PERMANOVA-S，p = 0.02）。加权UniFrac距离矩阵主坐标分析的前两轴评分与哮喘呈负相关。在尘埃样本中检测到的658个属中，41个基因的相对丰度与其中的一个轴相关（r> | 0.4 |）。乳球菌属是哮喘的危险因素（aOR 1.36,95％CI 1.13-1.63 / IQR变化）。 12个细菌属（主要来自放线菌目）的丰度与较低的哮喘风险相关（p <0.10），但并不相互独立。这12个相互关联的属的总相对丰度具有显著的保护作用，也一大部分解释了丰富性与较少哮喘发生的关联。
结论：我们的数据证实，婴儿家庭微生物群的系统差异与随后的哮喘风险相关，并且表明与个别细菌分类群或仅仅是丰富度相比，所选取的细菌群落与哮喘保护的关联性更高。
(中日友好医院呼吸与危重症医学科 顾宪民 摘译 林江涛 审校)
(J Allergy Clin Immunol. 2019 Aug 12. pii: S0091-6749(19)31033-4. doi: 10.1016/j.jaci.2019.07.035.)
(J Allergy Clin Immunol. 2019 Aug 12. pii: S0091-6749(19)31033-4. doi: 10.1016/j.jaci.2019.07.035.)
Indoor Bacterial Microbiota and the Development of Asthma by 10.5 years of age.
Karvonen AM, Kirjavainen PV, Täubel M, Jayaprakash B, Adams RI, Sordillo JE, Gold DR, Hyvärinen A, Remes S, von Mutius E, Pekkanen J.
Abstract
BACKGROUND:Early-life indoor bacterial exposure is associated with the risk of asthma but the roles of specific bacterial genera are poorly understood.
OBJECTIVE:To determine whether individual bacterial genera in indoor microbiota predict the development of asthma.
METHODS:Dust samples from living rooms were collected at 2 months of age. The dust microbiota was characterized by Illumina MiSeq sequencing amplicons of bacterial 16S ribosomal RNA gene. Children (N=373) were followed up for ever asthma until the age of 10.5 years.
RESULTS:Richness was inversely associated with asthma after adjustments (p=0.03). The phylogenetic microbiota composition in asthmatics' homes was characteristically different from non-asthmatics' homes (weighted UniFrac, adjusted association, PERMANOVA-S, p=0.02). The first two axis scores of principal coordinate analysis of the weighted UniFrac-distance matrix were inversely associated with asthma. Out of 658 genera detected in the dust samples, the relative abundances of 41genera correlated (r>|0.4|) with one of these axes. Lactococcus genus was a risk factor for asthma (aOR 1.36, 95%CI 1.13-1.63 per IQR change). The abundance of twelve bacterial genera (mostly from Actinomycetales order) was associated with lower asthma risk (p<0.10), though not independently of each other. The sum relative abundance of these12 intercorrelated genera was significantly protective and explained majority of the association of richness with less asthma.
CONCLUSIONS:Our data confirms that phylogenetic differences in infant home microbiota are associated with subsequent asthma risk and suggest that communities of selected bacteria are more strongly linked to asthma protection than individual bacterial taxon or mere richness.
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产前氧化平衡与青春期哮喘和过敏性疾病的风险
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葡萄球菌肠毒素的敏感性和哮喘严重程度：EGEA队列中的纵向研究
