家庭的兄弟姐妹与婴儿鼻腔和粪便菌群的关系
2016/11/09
摘要
背景：早期与较大兄弟姐妹一起生活的婴儿患哮喘的风险更低。到目前为止，还没有过有关兄弟姐妹对婴儿气道和和肠道菌群的影响的研究。我们旨在弄清婴儿的鼻腔和粪便菌群谱，并研究有兄弟姐妹和菌群分布之间的关系。
方法:我们进行了一项105名健康婴儿（小于1岁）的横断面研究。对鼻气道和粪便样本进行了16S rRNA基因测序，并使用无偏聚类方法，我们发现了菌群的分布，并确定了有兄弟姐妹和菌群分布之间的关系。
结果:总的来说，年龄中位数为3.4个月（IQR 2.0-4.7个月）；43%婴儿受试者的家中有兄弟姐妹。鼻气道微生物无偏聚类鉴定出3个菌群分布谱：摩拉克氏菌优势菌群（43%），棒状杆菌/ Dolosigranulum优势菌群（36%），和混合菌群（21%）。有兄弟姐妹的婴儿更可能有摩拉克氏菌优势菌群，而不是棒状杆菌/ Dolosigranulum优势菌群（76% VS. 18%）；而那些没有兄弟姐妹有相反的模式（18% VS. 50%）（多变量校正，P＜0.001）。粪便菌群包括3个菌群分布谱：双歧杆菌优势菌群（39%），大肠杆菌优势菌群（31%），和肠杆菌优势菌群（30%）。有兄弟姐妹的婴儿更可能有双歧杆菌优势菌群，而不是大肠杆菌优势菌群（49% VS. 24%）；而那些没有兄弟姐妹有相反的模式（32% VS. 37%）（多变量校正P = 0.04）。
结论：在这个横断面研究中，我们发现有兄弟姐妹的婴儿在鼻腔菌谱中更可能有摩拉克氏菌优势菌群，在肠道菌群中有双歧杆菌优势菌群分布。我们的发现可进一步加速早期生活环境暴露、微生物菌群、和儿童哮喘之间的相互作用的研究。
（苏欣 审校）
Pediatr Int. 2016 Sep 16. doi: 10.1111/ped.13168. [Epub ahead of print]
Association of household siblings with nasal and fecal microbiota in infants.
Hasegawa K1, Linnemann RW2, Mansbach JM3, Ajami NJ4, Espinola JA5, Fiechtner LG6, Petrosino JF4, Camargo CA Jr5.
Author information
Abstract
BACKGROUND:Early-life exposure to older siblings is associated with a lower risk of asthma. To date, no study has addressed the impact of having siblings on both the airway and fecal microbiota during infancy. We aimed to profile the nasal airway and fecal microbiota in infants, and to examine the association between having siblings and microbiota profiles.
METHODS:We conducted a cross-sectional study of 105 healthy infants (aged <1 year). By applying 16S rRNA gene sequencing and an unbiased clustering approach to the nasal airway and fecal samples, we identified microbiota profiles and then determined the association between having siblings and microbiome profiles.
RESULTS:Overall, the median age was 3.4 months (IQR, 2.0-4.7 months); 43% had siblings in the household. Unbiased clustering of nasal airway microbiota identified three profiles: Moraxella-dominant (43%), Corynebacterium/Dolosigranulum-dominant (36%), and mixed (21%). Infants with siblings were more likely to have Moraxella-dominant profile than Corynebacterium/Dolosigranulum-dominant profile (76% vs. 18%) while those without siblings had the opposite pattern (18% vs. 50%) (multivariable-adjusted P<0.001). Fecal microbiota consisted of three profiles: Bifidobacterium-dominant (39%), Escherichia-dominant (31%), and Enterobacter-dominant (30%). Infants with siblings were more likely to have Bifidobacterium-dominant profile than Escherichia-dominant profile (49% vs. 24%) while those without siblings had the opposite pattern (32% vs. 37%) (multivariable-adjusted P=0.04).
CONCLUSIONS:In this cross-sectional study, we found that infants with siblings were more likely to have Moraxella-dominant nasal microbiota profile and Bifidobacterium-dominant fecal microbiota profile. Our findings should facilitate further investigation of the interplay between early-life environmental exposures, the microbiome, and childhood asthma. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:airway microbiota; asthma; fecal microbiota; infants; sibling
Pediatr Int. 2016 Sep 16. doi: 10.1111/ped.13168. [Epub ahead of print]
