中国急性下呼吸道感染住院患儿中感染的1到4型人类副流感病毒
2016/07/27
摘要
背景：人类副流感病毒（HPIVs）是急性下呼吸道感染的重要原因（ALRTIs）。HPIV-4，一种新发现的病毒，最近已经发现它与急性下呼吸道感染相关。
方法：在2010年3月和2011年2月之间共收集了771例住院患儿的鼻咽分泌物。通过巢式PCR检测发现了HPIVs，通过RT-PCR和PCR检测发现了其它已知的呼吸道病毒。对所有扩增产物进行测序。
结果：148例（19.19%）患者中发现了HPIVs的样本，其中28例（3.63%）HPIV-4阳性，12例（1.55%）HPIV-1阳性，4例（0.51%）HPIV－2阳性，107例（13.87%）HPIV-3阳性。只有3例是不同类型的HPIV共同感染。所有人副流感病毒阳性的儿童都在5岁以下，其中大部分是1岁以内。只有HPIV-3的检出率在不同年龄间有显著性差异（χ2 = 29.648，P = 0.000）。HPIV-3和HPIV -4在夏天被检测出来。60例（40.54%）与其它呼吸道病毒共感染，其中人鼻病毒（HRV）是最常见的共同感染病毒。最常见的临床诊断为支气管肺炎，所有患者都有咳嗽；一些被HIPV-3和4感染的患者有气促和发绀。那些感染了HPIV-4和HPIV-3的患者之间的临床表现无显著性差异。HPIV-4的两个基因型是普遍的，虽然HPIV-4a为显性。
结论：HPIV-4是中国患有急性下呼吸道感染住院患儿的重要病毒。HRV是最重要的共感染病毒。HPIV-4两种基因型是普遍的，HPIV-4a 为显性。
（苏欣 审校）
J Med Virol. 2016 May 19. doi: 10.1002/jmv.24580. [Epub ahead of print]
Human parainfluenza virus types 1 to 4 in hospitalized children with acute lower respiratory infections in China.
Xiao NG1, Duan ZJ2, Xie ZP2, Zhong LL1, Zeng SZ1, Han-Huang1, Gao HC2, Zhang B1.
Author information
Abstract
BACKGROUND:Human parainfluenza viruses (HPIVs) are an important cause of acute lower respiratory tract infections (ALRTIs). HPIV-4, a newly identified virus, has been associated with severe ALRTIs recently.
METHODS:A total of 771 nasopharyngeal aspirate samples were collected from hospitalized children between March 2010 and February 2011. HPIVs were detected by Nest -PCR, and other known respiratory viruses were detected by RT-PCR and PCR. All amplification products were sequenced.
RESULTS:HPIVs were detected in 148 (19.19%) patients, of whom 28 (3.63%) were positive for HPIV-4, 12(1.55%) for HPIV-1, 4 (0.51%) for HPIV-2, and 107 (13.87%) for HPIV-3. Only 3 were found to be co-infected with different types of HPIVs. All HPIV-positive children were under 5 years of age, with the majority being less than 1 year. Only the detection rate of HPIV-3 had a significant statistical difference (χ2 = 29.648, P = 0.000) between ages. HPIV-3 and HPIV-4 were detected during the summer. Sixty (40.54%) were co-infected with other respiratory viruses, and human rhinovirus (HRV) was the most common co-infecting virus. The most frequent clinical diagnosis was bronchopneumonia, and all patients had cough; some patients who were infected with HPIV-3 and HPIV-4 had polypnea and cyanosis. No significant difference was found in clinical manifestations between those who were infected with HPIV-4 and HPIV-3. Two genotypes for HPIV-4 were prevalent, although HPIV-4a dominated.
CONCLUSION:HPIV-4 is an important virus for children hospitalized with ALRTIs in China. HRV was the most common co-infecting virus. Two genotypes for HPIV-4 are prevalent, HPIV-4a dominated. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:clustal analysis; human parainfluenza virus; respiratory tract; seasonal incidence
J Med Virol. 2016 May 19. doi: 10.1002/jmv.24580. [Epub ahead of print]
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