血清中血管生成素-2（Ang-2）浓度与重度哮喘表型相关
2016/05/26
摘要
背景：一些促血管生成的分子与哮喘炎症和重构的发病机理有密切联系。本研究的目的在比较哮喘病人和健康受试者（HS）血清中促血管生成因子的浓度，并把这种浓度作为疾病严重程度的临床和炎性标记物的相关参考。
方法：收集45例重症/难治性哮喘（SRA）和51例非重症哮喘（nSA）的血清，以30例HS作为对照组。血清中Ang-1、Ang-2、血管内皮生长因子（VEGF）和骨桥蛋白的浓度通过酶联免疫吸附实验进行评估。
结果：哮喘患者（n = 94）的血管生成素-1（Ang-1, 68.8±2.7 vs 56.4±9.3ng/ml；P<0.05），血管生成素-2（Ang-2, 4.9±0.35 vs 1.38±0.14ng/ml；P<0.0001）和VEGF的水平均明显高于HS（255±45.4 vs 424.5±27.8 pg /ml；P<0.01）。Ang-2的平均血清水平在SRA患者中显著高于nSA患者（6.04±0.46 vs 3.84±0.43；P<0.001）。Ang-2血清水平与呼吸功能和哮喘严重程度的参数相关，包括：在前12个月哮喘加重的平均次数（R = 0.21；P<0.05），严重哮喘发作的平均急救次数（R = 0.24；P<0.04），平均住院人数（R = 0.21；P<0.05）或患者吸入糖皮质激素的剂量（R = 0.36；P<0.001）。
结论：Ang-2可能是重要的促血管生成细胞因子，在反复加重的SRA病人身上过量表达。Ang-2血清水平可作为重度哮喘的一种生物标志物。
（苏欣 审校）
Allergy Asthma ClinImmunol. 2016 Mar 1;12:8.doi:10.1186/s13223-016-0112-6. eCollection 2016.
Angiopoietin-2 concentration in serum is associated with severe asthma phenotype.
Makowska JS1, Cieślak M2, Jarzębska M2, Lewandowska-Polak A3, Kowalski ML3.
Author information
Abstract
BACKGROUND:Several proangiogenic molecules have been implicated in the pathogenies of asthmatic inflammation and remodeling. The aim of the study was to compare the concentration of proangiogenic factors in the sera of asthmatic patients and in healthy subjects (HS), and to refer the concentrations to both clinical and inflammatory markers of the disease severity.
METHODS:Serum was collected from 45 patients with severe/refractory asthma (SRA) and 51 patients with non-severe asthma (nSA). The control group included 30 HS. Serum concentrations of Angiopoietin-1, Angiopoietin-2, vascular endothelial growth factor (VEGF) and osteopontin were assessed by the enzyme-linked immunosorbent assay.
RESULTS:The levels of Angiopoietin-1 (68.8 ± 2.7 vs 56.4 ± 9.3 ng/ml; p < 0.05), Angiopoietin-2 (4.9 ± 0.35 vs 1.38 ± 0.14 ng/ml; p < 0.0001) and VEGF were significantly higher in asthmatic patients (n = 94) as compared to HS (255 ± 45.4 vs 424.5 ± 27.8 pg/ml; p < 0.01). The mean serum level of Angiopoietin-2 was found to be significantly higher in patients with SRA as compared to nSA patients (6.04 ± 0.46 vs 3.84 ± 0.43; p < 0.001). Angiopoietin-2 serum level correlated with respiratory function and with parameters of asthma severity: the mean number of asthma exacerbations in the preceding 12 months (R = 0.21; p < 0.05), mean number of emergency visits due to severe asthma exacerbation (R = 0.24; p < 0.04) and mean number of hospitalizations (R = 0.21; p < 0.05) or dose of inhaled glucocorticosteroids taken by the patients (R = 0.36; p < 0.001).
CONCLUSION:Angiopoietin-2 seems to be a crucial proangiogenic cytokine overproduced in patients with SRA characterized by repeated exacerbations and Angiopoietin-2 serum levels can serve as a biomarker of severe asthma.
KEYWORDS:Angiopoietins; Pathogenesis; Severe asthma
Allergy Asthma Clin Immunol. 2016 Mar 1;12:8. doi:10.1186/s13223-016-0112-6. eCollection 2016.
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