哮喘联盟

   摘要
   玻连蛋白是一种多功能的糖蛋白，参与凝血、抑制膜攻击复合物（MAC）的形成、细胞粘附和迁移、伤口愈合和组织重构。玻连蛋白主要的细胞来源是肝细胞；虽然在健康受试者和间质性肺疾病患者的呼出气冷凝液和支气管肺泡灌洗液中发现糖蛋白的存在，但是呼吸道的固有细胞是否产生玻连蛋白并不清楚。哮喘和COPD患者中玻连蛋白的表达是否改变也不清楚。在本项研究中，尸检获得7例哮喘患者、10例COPD患者和14例对照受试者的支气管组织，并用免疫组化的方法检测含玻连蛋白的粘膜下腺所占的面积百分比。在一组单独的实验中，对供体（6例哮喘、4例COPD和7例对照）肺的气管支气管组织进行定量共定位分析。对12例进行诊断性支气管镜检查的受试者支气管表面上皮中玻连蛋白RNA和蛋白的表达情况进行检测。哮喘、COPD和对照受试者的气管支气管上皮中都发现玻连蛋白，但在哮喘患者中玻连蛋白的表达显著降低。三维共聚焦图片共定位分析表明玻连蛋白在浆液性上皮细胞和呼吸道上皮细胞表面表达，在杯状细胞无表达。哮喘和COPD患者支气管表面上皮65-KD玻连蛋白亚型的表达降低。引起哮喘患者玻连蛋白表达下降的原因并不明确，但呼吸道上皮/粘膜下层的玻连蛋白表达下降可能与气道重构有关。

（杨冬 审校）
PLoS One. 2015 Mar 13;10(3):e0119717. doi: 10.1371/journal.pone.0119717.
 

Vitronectin Expression in the Airways of Subjects with Asthma and Chronic Obstructive Pulmonary Disease.
 

Salazar-Peláez LM1, Abraham T2, Herrera AM1, Correa MA3, Ortega JE4, Paré PD5, Seow CY6.
 

ABSTRACT
Vitronectin, a multifunctional glycoprotein, is involved in coagulation, inhibition of the formation of the membrane attack complex (MAC), cell adhesion and migration, wound healing, and tissue remodeling. The primary cellular source of vitronectin is hepatocytes; it is not known whether resident cells of airways produce vitronectin, even though the glycoprotein has been found in exhaled breath condensate and bronchoalveolar lavage from healthy subjects and patients with interstitial lung disease. It is also not known whether vitronectin expression is altered in subjects with asthma and COPD. In this study, bronchial tissue from 7 asthmatic, 10 COPD and 14 control subjects was obtained at autopsy and analyzed by immunohistochemistry to determine the percent area of submucosal glands occupied by vitronectin. In a separate set of experiments, quantitative colocalization analysis was performed on tracheobronchial tissue sections obtained from donor lungs (6 asthmatics, 4 COPD and 7 controls). Vitronectin RNA and protein expressions in bronchial surface epithelium were examined in 12 subjects who undertook diagnostic bronchoscopy. Vitronectin was found in the tracheobronchial epithelium from asthmatic, COPD, and control subjects, although its expression was significantly lower in the asthmatic group. Colocalization analysis of 3D confocal images indicates that vitronectin is expressed in the glandular serous epithelial cells and in respiratory surface epithelial cells other than goblet cells. Expression of the 65-kDa vitronectin isoform was lower in bronchial surface epithelium from the diseased subjects. The cause for the decreased vitronectin expression in asthma is not clear, however, the reduced concentration of vitronectin in the epithelial/submucosal layer of airways may be linked to airway remodeling.
 

PLoS One. 2015 Mar 13;10(3):e0119717. doi: 10.1371/journal.pone.0119717.