儿童支气管哮喘发病机制的初步研究
2015/03/18
摘要
背景：支气管哮喘的病因学和发病机制尚不清楚。本研究旨在从遗传学和免疫学角度，探讨儿童支气管哮喘发病的相关风险因素，并初步揭示儿童支气管哮喘的发病机理。
方法：采用实时定量PCR技术，检测在两组儿童的外周静脉血血清中，TRPV1 基因 和mRNA的表达水平，酶联免疫吸附方法（ELISA法）检测总IgE、 IL-4、IL-5和IFN-γ水平。采用Logistic回归分析研究诱导儿童支气管哮喘的最重要因素。
结果：病例组外周血中的 TRPV1的mRNA 水平显著高于对照组（p < 0.01）。病例组血清中IL-4，IL-5和 EOS水平明显高于对照组（p < 0.01）， 然而IFN-γ水平明显低于对照组 （p < 0.01）。Logistic回归分析结果表明，TRPV1表达水平，IL-4水平和rs4790522位点突变是诱导儿童支气管哮喘的主要危险因素。
结论：TRPV1基因表达和Th1/Th2细胞因子的水平与儿童哮喘发病有着密切的关系，这为儿童支气管哮喘分子靶向治疗提供了理论依据。
（林江涛 审校）
Pediatr Res. 2015 Jan 13. doi: 10.1038/pr.2015.11. [Epub ahead of print]
Preliminary study on pathogenesis of bronchial asthma in children.
Ren YF1, Li H2, Xing XH2, Guan HS2, Zhang BA3, Chen CL4, Zhang JH5.
Abstract
BACKGROUND：Etiology and pathogenesis of bronchial asthma remain unclear. This study is to investigate risk factors related to bronchial asthma onset in children from genetics and immunology and preliminarily reveal pathogenesis of bronchial asthma in children.
METHODS：Real-time quantification PCR was adopted to detect expression level of TRPV1 gene and mRNA and ELISA method to the total IgE level and levels of IL-4, IL-5 and IFN-γ in serum in peripheral venous blood for children in two groups. Logistic regression analysis was applied to analyze the most essential factors inducing bronchial asthma in children.
RESULTS：mRNA level of TRPV1 in peripheral blood in case group was higher than that in control group (p<0.01). Levels of IL-4, IL-5 and EOS in serum in case group were markedly higher than those in control group (p<0.01), while IFN-γ level was lower than that in control group (p<0.01). Results of logistic regression analysis indicated that TRPV1 expression level, IL-4 level and rs4790522 site mutation were the main risk factors inducing bronchial asthma in children.
CONCLUSIONS：Levels of TRPV1 gene expression and Th1/Th2 cytokines have a close relationship with asthma onset in children, which provides theoretical evidences for molecular targeted treatment in children with bronchial asthma.
Pediatr Res. 2015 Jan 13. doi: 10.1038/pr.2015.11. [Epub ahead of print]
