哮喘联盟

   摘要
   我们之前的研究结果表明，普诺维洛尔（一种多酚化合物）对于过敏性炎症具有抗炎作用。本项目旨在研究普诺维洛尔及联合使用临床止哮药（布地奈德或茶碱）在实验性过敏性哮喘中对气道防御机制的作用。各组豚鼠在21天卵清蛋白致敏过程中分别给予普诺维洛尔（20 mg/kg/天, 口服）、布地奈德（1 mM吸入）、茶碱（10 mg/kg/天, 腹膜腔内注射）、普诺维洛尔与布地奈德的半剂量联合、普诺维洛尔与茶碱的半剂量联合的治疗。气道防御机制通过体内咳嗽反射以及特异性气道阻力（sRaw）等指标来评估。同时，在体外检测气管平滑肌反应性及气道粘液纤毛清除功能。研究结果显示，普诺维洛尔可导致特异性气道阻力以及气管平滑肌收缩性显著下降，此外，它还可以抑制咳嗽反射，并对纤毛摆动频率进行正向的调节。普诺维洛尔的支气管扩张效果及止喘效果与临床上使用的布地奈德或茶碱相似。虽然在联合治疗措施中布地奈德及茶碱的治疗剂量已经减半，但普诺维洛尔作为一种附加治疗措施可显著加强布地奈德及茶碱的治疗效果。因此，普诺维洛尔不仅有支气管扩张及止喘的效果，还可以加强布地奈德及茶碱的类似效果。
 

（苏楠 审校）
Adv Exp Med Biol. 2014 Oct 15. [Epub ahead of print]

 

Effects of Provinol and Its Combinations with Clinically Used Antiasthmatics on Airway Defense Mechanisms in Experimental Allergic Asthma.
 

Kazimierová I1, Jošková M, Pecháňová O, Sutovská M, Fraňová S.
 

ABSTRACT
Our previous studies show that provinol, a polyphenolic compound, has anti-inflammatory activity during allergic inflammation. In the present study we investigated the effects of provinol and its combinations with clinically used antiasthmatics: budesonide or theophylline on airway defense mechanisms during experimental allergic asthma. Separate groups of guinea pigs were treated during the course of 21-day ovalbumin sensitization with provinol (20 mg/kg/day, p.o.), or budesonide (1 mM by inhalation), or theophylline (10 mg/kg/day, i.p.), and with a half-dose combination of provinol+budesonide or provinol+theophylline. Airways defense mechanisms: cough reflex and specific airway resistance (sRaw) were evaluated in vivo. Tracheal smooth muscle reactivity and mucociliary clearance were examined in vitro. The findings were that provinol caused significant decreases in sRaw and in tracheal smooth muscle contractility, a suppression of cough reflex, and positively modulated ciliary beat frequency. The bronchodilatory and antitussive effects of provinol were comparable with those of budesonide and theophylline. Provinol given as add-on treatment significantly potentiated the effects of budesonide or theophylline, although the doses of each were halved. We conclude that provinol not only has bronchodilatory and antitussive effects, but also potentiates similar effects exerted by budesonide and theophylline.
 

Adv Exp Med Biol. 2014 Oct 15. [Epub ahead of print]