哮喘联盟

   摘要
   持续或反复皮肤屏障破坏后发生的特应性皮炎（AD）是特异性反应的开始，并将最终演变为呼吸道过敏。维持角质层（SC）pH值酸性是皮肤屏障发挥正常功能的重要因素。本研究旨在研究局部应用/经鼻吸入欧洲屋尘螨（Dp）是否会使恶唑酮（Ox）诱导AD小鼠模型发生气道炎症——一种新型特异性反应动物模型，并探索反复使用酸性乳膏创造角质层酸性环境是否可阻断这一特异性反应。在反复使用Ox及Dp诱导特异性小鼠模型时，对相应小鼠使用酸性乳膏（pH 2.8）或中性乳膏(pH 7.4)（每天两次），乳膏的pH值通过柠檬酸及氢氧化钠进行调节并与载体混合。反复对无毛小鼠使用Ox和Dp诱导产生AD样病变后，小鼠发生呼吸道过敏，证实特异性反应动物模型构建成功。而酸性乳膏可阻止气道过敏性炎症和AD样皮肤病变的发生。这些结果证实，通过对Ox诱导的AD小鼠模型反复局部及经鼻给予屋尘螨，可建立一种新型特异性反应动物模型，而酸化角质层可作为一种阻断特异性反应的新型干预方式。
 

（苏楠 审校）
ExpDermatol. 2014 Jul 30.doi: 10.1111/exd.12525. [Epub ahead of print]

 

Topical Acidic Cream Prevents The Development of Atopic Dermatitis- and Asthma-Like Lesions in Murine Model.
 

Lee HJ1, Yoon NY, Lee NR, Jung M, Kim DH, Choi EH.
 

ABSTRACT
Long-standing or repeated skin barrier damage followed by atopic dermatitis (AD) is the initial step of the atopic march that eventually progresses to respiratory allergies. Maintenance of an acidic pH in the stratum corneum (SC) is an important factor for normal skin barrier function. We performed this study in order to determine whether an oxazolone (Ox)-induced AD murine model can develop airway inflammation by topical application and nasal inhalation of a house dust mite, Dermatofagoidespteronyssinus (Dp), which is a novel 'atopic march animal model', and whether an acidic SC environment, made by repeated application of acidic cream, can interrupt this atopic march. During repeated treatment with Ox and Dp to make an atopic march murine model, acidic cream (pH 2.8) and neutral cream (pH 7.4) adjusted by citric acid and sodium hydroxide mixed with vehicle were applied twice daily. Repeated treatment with Ox and Dp to hairless mice induced AD-like skin lesions followed by respiratory allergy, defining it as an atopic march model. Acidic cream inhibited the occurrence of respiratory allergic inflammation as well as AD-like skin lesions. These results indicate that a novel atopic march animal model can be developed by repeated topical and nasal treatments with house dust mite on Ox-induced AD mice, and that the acidification of SC could be a novel intervention method to block the atopic march.
 

ExpDermatol. 2014 Jul 30.doi: 10.1111/exd.12525. [Epub ahead of print]