哮喘联盟

   摘要
   哮喘气道炎症的发病机制涉及表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）、血小板源生长因子（PDGF）的AA和BB亚型及血管内皮生长因子。本研究的研究内容为哮喘表型与诱导痰液和血清中的这些生长因子表达水平的相关性。根据痰液的细胞学分类，62例哮喘患者被分为嗜酸性粒细胞和中性粒细胞表型。另外，还根据肺功能(FEV1/FVC >70%或 FEV1/FVC <70%)和哮喘严重程度（轻度、重度和重度）对患者进行分类。血清和痰液中的EGF, bFGF, PDGF-AA, PDGF-BB和VEGF浓度通过酶联免疫分析进行测定。与中性粒细胞表型哮喘患者相比，嗜酸性粒细胞表型哮喘患者血清和诱导痰液中的VEGF水平更高。与FEV1/FVC >70%的哮喘患者相比，FEV1/FVC <70%的哮喘患者的VEGF表达水平更高。与轻-中度哮喘患者相比，重度哮喘患者的VEGF水平更高。不同表型哮喘患者之间EGF、bFGF、PDGF-AA和 PDGF-BB水平的差异无统计学意义。因此，本研究的结果表明：哮喘患者血清和诱导痰液中VEGF表达水平的增加可能与嗜酸性粒细胞性气道炎症、严重气流受限和哮喘的严重程度相关。
 

（林江涛 审校）
Exp Ther Med. 2014 Aug;8(2):573-578. Epub 2014 Jun 4.

 

Analysis of growth factors in serum and induced sputum from patients with asthma.
 

Zou H1, Fang QH2, Ma YM2, Wang XY1.
 

ABSTRACT
Epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), the AA and BB isoforms of platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) are involved in the pathogenesis of airway inflammation in asthma. In the present study, the associations between asthmatic phenotypes and the expression levels of these mediators in induced sputum and serum were investigated. A total of 62 asthmatic patients were divided into eosinophilic or neutrophilic phenotypes by cytological classification of the induced sputum. In addition, patients were classified according to lung function (FEV1/FVC >70% or FEV1/FVC <70%) and asthma severity (mild, moderate or severe). The concentrations of EGF, bFGF, PDGF-AA, PDGF-BB and VEGF in the serum and induced sputum were measured using sandwich enzyme immunoassays. VEGF levels in the serum and induced sputum were higher in patients with an eosinophilic phenotype compared with those with a neutrophilic phenotype. In addition, VEGF expression was higher in patients with an FEV1/FVC value of <70% as compared with patients with an FEV1/FVC value of >70%. Furthermore, the levels of VEGF were higher in patients with severe asthma compared with the patients with mild and moderate asthma. There were no statistically significant differences observed with regard to EGF, bFGF, PDGF-AA and PDGF-BB levels among the various phenotypes. Therefore, the observations of the present study indicated that increased VEGF expression in the serum and induced sputum of patients may be associated with eosinophilic airway inflammation, severe airflow limitation and the severity of asthma.
 

Exp Ther Med. 2014 Aug;8(2):573-578. Epub 2014 Jun 4.