小鼠哮喘模型中气道改变的显微CT定量研究与病理结果比较
2014/03/06
摘要
目的:依据尸检显微CT影像以及病理结果评估卵清蛋白诱导哮喘小鼠的气道改变。
方法:一组小鼠通过腹腔注射和鼻滴入乳清蛋白氢氧化铝诱导的哮喘(实验组, n=6),另一组小鼠接受腹腔注射和鼻滴入蒸馏磷酸盐缓冲盐水(对照组, n=6)。使用Lucion智能笔(半自动)和曲线笔(手动),测定显微CT轴向扫描所显示的远端第三支气管分支水平段(每只小鼠分6段)主支气管腔面积。在多维平面重建(MPR)曲线影像显示下,通过测定与主支气管纵轴相垂直的直径,获取第三支气管分支水平段后的4节段(每侧2段)支气管管壁厚度。组织学切片是从与其CT影像相匹配的病灶处获得,并测定支气管壁厚度。
结果:平均支气管管腔面积实验组为0.196±0.072 mm(2),对照组为0.243±0.116 mm(2);差异具有显著的统计学意义。与对照组相比,实验组在显微CT影像 (平均值, 0.119±0.01 vs. 0.108±0.013 mm)和病理标本(平均值, 0.066±0.011 vs. 0.041±0.009 mm)上支气管壁厚度更厚;显微CT影像上支气管壁厚度与病理学厚度具有很好的相关性(实验组, r=0.712; 对照组, r=0.46)。实验组中厚支气管壁显示粘膜下层肥大并伴有杯状细胞和平滑肌细胞增生。
结论:显微CT影像显示哮喘可以诱导支气管壁增厚以及管腔面积变窄并与病理检查结果显著相关。
(林江涛 审校)
Allergy Asthma Immunol Res.2014 Jan;6(1):75-82.doi:10.4168/aair.2014.6.1.75. Epub 2013 Dec 26.
A Quantitative Study of Airway Changes on Micro-CT in a Mouse Asthma Model: Comparison With Histopathological Findings.
Paik SH1, Kim WK1, Park JS1, Park CS2, Jin GY3.
Abstract
PURPOSE: To evaluate airway changes in ovalbumin-induced asthmatic mice in terms of postmortem micro-CT images and pathological findings.
METHODS: Asthma was induced in mice by intraperitoneal injection and nasal instillation of ovalbumin aluminium hydroxide into mice (experimental group, n=6), and another group of mice received intraperitoneal injection and nasal instillation of distilled phosphate-buffered saline (control group, n=6). Bronchial lumen area was measured in the main bronchial lumen of the distal third bronchial branch level (6 parts per each mouse) on axial scans of Micro-CT, using a Lucion's smart pen (semi-automated) and a curve pen (manual). Bronchial wall thickness was obtained in 4 sections (2 levels on either side) after the third bronchial branch by measuring the diameter which was perpendicular to the longitudinal axis of the main bronchus on curved Multi-planar reconstruction (MPR) images. Histologic slides were obtained from the lesion that was matched with its CT images, and bronchial wall thicknesses were determined.
RESULTS: The mean bronchial lumen area was 0.196±0.072 mm(2) in the experimental group and 0.243±0.116 mm(2) in the control group; the difference was significant. Bronchial wall thickness on micro-CT images (mean, 0.119±0.01 vs. 0.108±0.013 mm) and in pathological specimens (mean, 0.066±0.011 vs. 0.041±0.009 mm) were thicker in the experimental group than in the control group; bronchial wall thickness on micro-CT images correlated well with pathological thickness (for the experimental group, r=0.712; for the control group, r=0.46). The thick bronchial wall in the experimental group demonstrated submucosal hypertrophy along with goblet cell hyperplasia and smooth muscle hyperplasia.
CONCLUSIONS: The results of this study suggest that asthma may induce thickening of bronchial wall and narrowing of the lumen area on micro-CT images and that these results may significantly correlate with pathological findings.
Allergy Asthma Immunol Res. 2014 Jan;6(1):75-82. doi: 10.4168/aair.2014.6.1.75. Epub 2013 Dec 26.
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危重哮喘综合征的免疫生物学
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组胺释放因子和免疫球蛋白在哮喘和变态反应中的作用