四种止咳药对暴露于香烟烟雾下的慢性咳嗽豚鼠模型神经性炎症的影响
2013/12/04
摘要
目的：四种止咳药包括磷酸可待因(CP),莫吉斯坦,左羟丙哌嗪 (LVDP)和柚苷对慢性咳嗽豚鼠的气道神经性炎症和咳嗽加剧的影响。
方法：豚鼠暴露于香烟烟雾下达8周。在第7周和第8周时，分别接受CP (4.8 mg/kg),莫吉斯坦(24 mg/kg), LVDP (14 mg/kg)和柚苷(18.4 mg/kg)治疗。之后，记录在辣椒素诱导期间咳嗽和时间进展的曲线下面积(Penh-AUC)。同时评估肺中的P物质 (SP)含量，NK-1受体表达和中性肽内切酶(NEP)活动度。
结果：慢性香烟烟雾暴露诱导辣椒素双相位时间的咳嗽反应。8周的香烟烟雾暴露显著增强豚鼠气道神经性炎症和咳嗽反应。CP, 莫吉斯坦, LVDP或柚苷2周治疗，有效改善慢性香烟暴露引起的咳嗽加剧。只有柚苷显著影响Penh-AUC、 SP 含量和 NK-1 受体表达，有效防止肺NEP活动度的下降。
结论：长时间暴露于香烟烟雾的豚鼠适用于慢性病理性咳嗽的研究，柚苷能有效的抑制气道神经性炎症和咳嗽加剧。
（刘国梁 审校）
Inflamm Res. 2013 Oct 2. [Epub ahead of print]
Effects of four antitussives on airway neurogenic inflammation in a guinea pig model of chronic cough induced by cigarette smoke exposure.
Luo YL, Li PB, Zhang CC, Zheng YF, Wang S, Nie YC, Zhang KJ, Su WW.
ABSTRACT
OBJECTIVE: The effects of four antitussives, including codeine phosphate (CP), moguisteine, levodropropizine (LVDP) and naringin, on airway neurogenic inflammation and enhanced cough were investigated in guinea pig model of chronic cough.
METHODS: Guinea pigs were exposed to CS for 8 weeks. At the 7th and 8th week, the animals were treated with vehicle, CP (4.8 mg/kg), moguisteine (24 mg/kg), LVDP (14 mg/kg) and naringin (18.4 mg/kg) respectively. Then the cough and the time-enhanced pause area under the curve (Penh-AUC) during capsaicin challenge were recorded. The substance P (SP) content, NK-1 receptor expression and neutral endopeptidase (NEP) activity in lung were determined.
RESULTS: Chronic CS exposure induced a bi-phase time course of cough responsiveness to capsaicin. Eight weeks of CS exposure significantly enhanced the airway neurogenic inflammation and cough response in guinea pigs. Two weeks of treatment with CP, moguisteine, LVDP or naringin effectively attenuated the chronic CS-exposure enhanced cough. Only naringin exerted significant effect on inhibiting Penh-AUC, SP content and NK-1 receptor expression, as well as preventing the declining of NEP activity in lung.
CONCLUSIONS: Chronic CS-exposed guinea pig is suitable for studying chronic pathological cough, in which naringin is effective on inhibiting both airway neurogenic inflammation and enhanced cough.
Inflamm Res. 2013 Oct 2. [Epub ahead of print]
