哮喘患者痰上层清液中的血管内皮生长因子和半胱氨酰白三烯
2013/11/13
摘要
背景：血管内皮生长因子(VEGF)在哮喘中被认为是生成血管的最重要因子。半胱氨酰白三烯(Cyst-LTs)在哮喘中已经被认为与血管通透性有关。Cyst-LTs受体拮抗剂通过降低VEGF表达调节血管通透性。
目的：旨在确定哮喘患者痰上层清液中VEGF和Cyst-LTs的水平，研究VEGF和Cyst-LTs之间可能的关系和与气道血管通透性指数的关系。同时评估其他可能的混杂因子。
方法：121例哮喘患者（38例重症难治性哮喘患者，41例吸烟者）和30例健康受试者（15例吸烟者）参加研究。所有受试者均接受肺功能检测，评估诱导痰中的细胞计数，痰上层清液中VEGF和Cyst-LTs水平，以及AVP指数。
结果：与正常受试者相比，哮喘患者的VEGF和Cyst-LTs (pg/ml) 水平均显著增加 (中位数, 五分位差分别为845 [487-1034] vs. 432 (327-654) 和 209 [171-296] vs. 92 [75-114] ，两者p < 0.001)。整个组中多变量回归分析表明，痰上层清液中Cyst-LTs水平与VEGF和AVP指数显著相关。痰上层清液中VEGF水平和AVP指数也有相似的正相关性。重症哮喘的出现在两者的关联中是一个重要的协变量。
结论：结果表明，Cyst-LTs可能通过上调VEGF表达调节血管通透性。以上的作用似乎受哮喘严重程度的影响。
Vascular endothelial growth factor and cysteinyl leukotrienes in sputum supernatant of patients with asthma.
Papadaki G, Bakakos P, Kostikas K, Hillas G, Tsilogianni Z, Koulouris NG, Papiris S, Loukides S.
ABSTRACT
BACKGROUND: Vascular endothelial growth factor (VEGF) is considered to be the most important angiogenic factor in asthma. Cysteinyl leukotrienes (Cyst-LTs) have been implicated in vascular permeability in asthma. Cyst-LTs receptor antagonists modulate vascular permeability by reducing VEGF expression.
OBJECTIVE: We aimed to determine the levels of VEGF and Cyst-LTs in sputum supernatants of patients with asthma and to investigate possible associations within them and with airway vascular permeability (AVP) index. Possible confounding factors were also assessed.
METHODS: One hundred twenty one patients with asthma (38 with severe refractory asthma, 41 smokers) and 30 healthy subjects (15 smokers) were studied. All subjects underwent lung function tests, and sputum induction for cell count identification and VEGF, Cyst-LTs, measurement in supernatants. AVP index was also assessed.
RESULTS: Both VEGF & Cyst-LTs (pg/ml) levels were significantly elevated in patients with asthma compared to normal subjects (median, interquartile ranges 845 [487-1034] vs. 432 (327-654) and 209 [171-296] vs. 92 [75-114] respectively, p < 0.001 for both). Multivariate regression analysis in the whole group showed a significant association of Cyst-LTs levels in sputum supernatants with VEGF levels in sputum supernatants and AVP index. A similar positive association was observed between VEGF levels in sputum supernatants and AVP index. The presence of Severe asthma was a significant covariate for both associations.
CONCLUSION: Our results indicate that Cyst-LTs may modulate vascular permeability by up-regulating VEGF expression. The above effect seems to be affected by asthma severity.
Respir Med. 2013 Sep;107(9):1339-45. doi: 10.1016/j.rmed.2013.06.014. Epub 2013 Jul 8.
背景：血管内皮生长因子(VEGF)在哮喘中被认为是生成血管的最重要因子。半胱氨酰白三烯(Cyst-LTs)在哮喘中已经被认为与血管通透性有关。Cyst-LTs受体拮抗剂通过降低VEGF表达调节血管通透性。
目的：旨在确定哮喘患者痰上层清液中VEGF和Cyst-LTs的水平，研究VEGF和Cyst-LTs之间可能的关系和与气道血管通透性指数的关系。同时评估其他可能的混杂因子。
方法：121例哮喘患者（38例重症难治性哮喘患者，41例吸烟者）和30例健康受试者（15例吸烟者）参加研究。所有受试者均接受肺功能检测，评估诱导痰中的细胞计数，痰上层清液中VEGF和Cyst-LTs水平，以及AVP指数。
结果：与正常受试者相比，哮喘患者的VEGF和Cyst-LTs (pg/ml) 水平均显著增加 (中位数, 五分位差分别为845 [487-1034] vs. 432 (327-654) 和 209 [171-296] vs. 92 [75-114] ，两者p < 0.001)。整个组中多变量回归分析表明，痰上层清液中Cyst-LTs水平与VEGF和AVP指数显著相关。痰上层清液中VEGF水平和AVP指数也有相似的正相关性。重症哮喘的出现在两者的关联中是一个重要的协变量。
结论：结果表明，Cyst-LTs可能通过上调VEGF表达调节血管通透性。以上的作用似乎受哮喘严重程度的影响。
（苏楠 审校）
Respir Med. 2013 Sep;107(9):1339-45. doi: 10.1016/j.rmed.2013.06.014. Epub 2013 Jul 8.
Respir Med. 2013 Sep;107(9):1339-45. doi: 10.1016/j.rmed.2013.06.014. Epub 2013 Jul 8.
Vascular endothelial growth factor and cysteinyl leukotrienes in sputum supernatant of patients with asthma.
Papadaki G, Bakakos P, Kostikas K, Hillas G, Tsilogianni Z, Koulouris NG, Papiris S, Loukides S.
ABSTRACT
BACKGROUND: Vascular endothelial growth factor (VEGF) is considered to be the most important angiogenic factor in asthma. Cysteinyl leukotrienes (Cyst-LTs) have been implicated in vascular permeability in asthma. Cyst-LTs receptor antagonists modulate vascular permeability by reducing VEGF expression.
OBJECTIVE: We aimed to determine the levels of VEGF and Cyst-LTs in sputum supernatants of patients with asthma and to investigate possible associations within them and with airway vascular permeability (AVP) index. Possible confounding factors were also assessed.
METHODS: One hundred twenty one patients with asthma (38 with severe refractory asthma, 41 smokers) and 30 healthy subjects (15 smokers) were studied. All subjects underwent lung function tests, and sputum induction for cell count identification and VEGF, Cyst-LTs, measurement in supernatants. AVP index was also assessed.
RESULTS: Both VEGF & Cyst-LTs (pg/ml) levels were significantly elevated in patients with asthma compared to normal subjects (median, interquartile ranges 845 [487-1034] vs. 432 (327-654) and 209 [171-296] vs. 92 [75-114] respectively, p < 0.001 for both). Multivariate regression analysis in the whole group showed a significant association of Cyst-LTs levels in sputum supernatants with VEGF levels in sputum supernatants and AVP index. A similar positive association was observed between VEGF levels in sputum supernatants and AVP index. The presence of Severe asthma was a significant covariate for both associations.
CONCLUSION: Our results indicate that Cyst-LTs may modulate vascular permeability by up-regulating VEGF expression. The above effect seems to be affected by asthma severity.
Respir Med. 2013 Sep;107(9):1339-45. doi: 10.1016/j.rmed.2013.06.014. Epub 2013 Jul 8.
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哮喘和COPD中血管重塑标志物的表达与缓激肽受体的关系
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哮喘风险以及其相关并发症的增加与儿童期肥胖的关系
