哮喘联盟

   摘要
   背景：玄参科是一种生长在伊朗东北部的植物，被作为传统的药草用于各种炎性疾病的治疗。本研究旨在探讨玄参提取物在卵白蛋白(OVA)诱导的小鼠哮喘模型中的保护作用
   方法：分别使用两种剂量(100和200毫克/公斤)的玄参提取物治疗OVA敏化的小鼠8到14天，在最后一次OVA刺激后48小时收集支气管肺泡灌洗液(BALF)，然后使用显微镜直接计数法评估嗜酸细胞和其他炎症细胞的数量。同时使用酶联免疫吸附试验测定BALF中的血清总(Ig) E、OVA特异性IgE和IL-4 、IL-5 细胞因子。另外，还使用薄层层析法(TLC)评估植化素和2,2-二苯基-1-苦肼基自由基(DPPH)评估主要化合物和植物提取物的抗氧化能力。
   结果：结果表明,主要化合物包括黄酮类、酚类化合物和苯丙烷类都存在于玄参提取物中。而且，随着提取物的显著减少BALF中的炎症细胞和Th2抑制因子如IL-4 和IL-5因子的数量也随之减少。总IgE和OVA-特异性IgE水平也随之减少。
   结论：总而言之，提取物具有调节Th2细胞因子的可能性，可以作为治疗过敏性哮喘的免疫调节剂。

 

（刘国梁 审校）
Daru. 2013 Jul 9;21(1):56. [Epub ahead of print]

 

Protective effects of Scrophularia striata in Ovalbumin-induced mice asthma model.
 

Azadmehr A, Hajiaghaee R, Zohal MA, Maliji G.
 

Abstract
BACKGROUND:Scrophularia striata Boiss. (Scrophulariaceae) is a plant growing in the northeastern part of Iran and being used as a traditional herb for various inflammatory disorders.This study was designed to investigate the protective effects of the Scrophularia striata extract in Ovalbumin (OVA) induced-asthma mice model.
METHODS:OVA-sensitized mice were intrapritonealy treated with two doses (100 and 200 mg/kg) of the extract on days 8 to 14 separately. Broncoalveolar lavage fluids (BALF) was collected 48 h after the final OVA challenge and then the number of eosinophils and other inflammatory cells were assessed by direct microscopic counting. In addition, total immunoglubolin (Ig) E and OVA-specific IgE levels in serum, IL-4 and IL-5 cytokines in BALF were determined by Enzyme-Linked Immunosorbent Assay. Moreover, phytochemical assay by thin layer chromatography (TLC) and the 2, 2 diphenyl-1-picrylhydrazyl (DPPH) were used to evaluate the main compounds and the antioxidant capacity of the plant extract, respectively.
RESULTS:The results showed that the main components; including flavonoids, phenolic compounds and phenyl propanoids were presented in the S. striata extract. In addition, the treatment with extract significantly reduced the number of inflammatory cells and suppressed T-helper 2 (Th2) cytokines including IL-4 and IL-5 in BALF. Also, total IgE and OVA-specific IgE levels in the serum decreased.
CONCLUSION:Collectively, it is concluded that the extract has the potential to modulate the Th2 cytokines and could be used as immunomodulatory agent in the treatment of allergic asthma.
 

Daru. 2013 Jul 9;21(1):56. [Epub ahead of print]