哮喘联盟

   摘要
   我们比较了实验性过敏性哮喘中，骨髓来源的单核细胞（BMMCs）与间充质细胞（MSCs）在气道炎症和重构及肺机制中的作用。用卵清蛋白对C57BL/6大鼠（OVA组）进行致敏和激发试验。对照组以生理盐水同样处理。末次激发后24小时，两组均进一步随机分为三个亚组，分别予以生理盐水、BMMCs (2×106)或MSCs(1×105)气管内注射。与OVA-SAL组相比，BMMCs和MSCs注射能降低细胞浸润、气管收缩指数、肺泡塌陷、肺泡隔胶原纤维成分，以及白介素-4、白介素-13、转化生长因子-β、血管内皮生长因子等因子浓度水平。BMMC比MSC更能改善肺功能、肺泡塌陷、肺泡隔胶原纤维成分和TGF-β、VEGF水平。结论， BMMCs 和MSCs 气管内注射能有效调节实验性哮喘模型中炎症和纤维化，同时，BMMC与纤维化相关生长因子的降低更为相关。
 

（苏楠 审校）
RespirPhysiolNeurobiol.2013Mar30.pii:S1569-9048(13)00093-1.doi:10.1016/j.resp.2013.03.014. [Epub ahead of print]

 

Bone marrow-derived mononuclear cells vs. mesenchymal stromal cells in experimental allergic asthma.

Abreu SC, Antunes MA, de Castro JC, de Oliveira MV, Bandeira E, Ornellas DS, Diaz BL, Morales MM, Xisto DG, Rocco PR.

Abstract
We compared the effects of bone marrow-derived mononuclear cells (BMMCs) and mesenchymal stromal cells (MSCs) on airway inflammation and remodeling and lung mechanics in experimental allergic asthma. C57BL/6 mice were sensitized and challenged with ovalbumin (OVA group). A control group received saline using the same protocol. Twenty-four hours after the last challenge, groups were further randomized into subgroups to receive saline, BMMCs (2×106) or MSCs (1×105) intratracheally. BMMC and MSC administration decreased cell infiltration, bronchoconstriction index, alveolar collapse, collagen fiber content in the alveolar septa, and interleukin (IL)-4, IL-13, transforming growth factor (TGF)-β and vascular endothelial growth factor (VEGF) levels compared to OVA-SAL. Lung function, alveolar collapse, collagen fiber deposition in alveolar septa, and levels of TGF-β and VEGF improved more after BMMC than MSC therapy. In conclusion, intratracheal BMMC and MSC administration effectively modulated inflammation and fibrogenesis in an experimental model of asthma, but BMMCs was associated with greater benefit in terms of reducing levels of fibrogenesis-related growth factors.

RespirPhysiolNeurobiol.2013Mar30.pii:S1569-9048(13)00093-1.doi:10.1016/j.resp.2013.03.014. [Epub ahead of print]