哮喘联盟

   摘要
   背景：调节性T细胞（Treg）在平衡免疫应答过程中起到重要作用，而Treg细胞功能障碍则参与了多种疾病的致病过程，包括哮喘。本研究主要在哮喘和非哮喘儿童的外周血中检测Treg细胞出现的频率。此外，还研究Treg细胞出现频率与过敏原致敏、疾病严重程度和药物治疗之间的关系。
   方法：分离健康对照者（n=93）和不同严重程度的哮喘患儿（n=66）的外周血单核细胞，使用多参数流式细胞仪进行检测。
   结果：我们的研究结果显示，哮喘患儿的Treg细胞出现频率较非哮喘儿童有所增加。多变量模型显示，哮喘患儿的Treg细胞出现频率直接与激素使用有关，但与哮喘严重程度、过敏原致敏和哮喘特应性状态无关。
   结论：低剂量、局部气道激素治疗足以影响外周血Treg细胞的出现频率。结果表明，研究Treg细胞时有必要考虑药物治疗的影响，哮喘患者激素吸入治疗可能可以通过增加Treg细胞出现频率来改善哮喘控制水平。
 

（林江涛 审校）
Clin Mol Allergy. 2013 Jan 25;11(1):1. [Epub ahead of print]

 

Inhaled corticosteroid use is associated with increased circulating t regulatory cells in children with asthma.
 
Singh AM, Dahlberg P, Burmeister K, Evans MD, Gangnon R, Roberg KA, Tisler C, Dasilva D,Pappas T, Salazar L, Lemanske RF Jr, Gern JE, Seroogy CM.

Abstract
BACKGROUND: T regulatory (Treg) cells are important in balancing immune responses and dysregulation of Treg cells has been implicated in the pathogenesis of multiple disease states including asthma. In this study, our primary aim was to determine Treg cell frequency in the peripheral blood of children with and without asthma. The secondary aim was to explore the association between Treg cell frequency with allergen sensitization, disease severity and medication use.
METHODS: Peripheral blood mononuclear cells from healthy control subjects (N = 93) and asthmatic children of varying disease severity (N = 66) were characterized by multi-parameter flow cytometry.
RESULTS: Our findings demonstrate that children with asthma had a significantly increased frequency of Treg cells compared to children without asthma. Using a multivariate model, increased Treg cell frequency in children with asthma was most directly associated with inhaled corticosteroid use, and not asthma severity, allergic sensitization, or atopic status of the asthma.
CONCLUSION: We conclude that low dose, local airway administration of corticosteroids is sufficient to impact the frequency of Treg cells in the peripheral blood. These data highlight the importance of considering medication exposure when studying Treg cells and suggest inhaled corticosteroid use in asthmatics may improve disease control through increased Treg cell frequency.
 

Clin Mol Allergy. 2013 Jan 25;11(1):1. [Epub ahead of print]