哮喘联盟

   摘要
   背景：人外周血细胞存在香草素瞬时受体电位2(TRPV2)，说明其在某些病理环境下具有一定作用。本试验在华北地区研究TRPV2基因表达与儿童期哮喘的关系。同时还研究了过敏原暴露对TRPV2表达的影响。
   方法：60名医生诊断为哮喘的儿童和60名健康儿童对照者入选本研究。检测血清总IgE和特异性IgE。采用qRT-PCR检测外周血淋巴细胞TRPV2的表达。Student's t检验和卡方检验用于分析TRPV2转录与不同研究变量之间关系对儿童期哮喘易感性的影响。多元logistic回归用于分析TRPV2基因与不同过敏原的相关性。
   结果：与对照者相比，哮喘患儿的TRPV2表达增加2.6倍（p < .01）。TRPV2表达上调和对三种过敏原（春季花粉、尘螨和狗/猫毛发）中任何一个的致敏与儿童期哮喘相关。此外，对春季花粉、蟑螂和尘螨的高反应性以及TRPV2表达上调是北京儿童哮喘发生的危险因素。
   结论：外周淋巴细胞TRPV2表达上调与华北儿童期哮喘密切相关。本研究为儿童期哮喘提供了一个潜在的生物标志物，为未来阐明哮喘致病机制提供了基础。
 

（刘国梁 审校）
J Asthma. 2013 Jan 7. [Epub ahead of print]

 

Transient Receptor Potential Vanilloid 2 (TRPV2), a Potential Novel Biomarker in Childhood Asthma.
 
Cai X, Yang YC, Wang JF, Wang Q, Gao J, Fu WL, Zhu ZY, Wang YY, Zou MJ, Wang JX, Xu DQ, Xu DG.

Source
Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences , Beijing , China.

Abstract 
BACKGROUND：The presence of transient receptor potential vanilloid 2 (TRPV2) in human peripheral blood cells may suggest a role under pathological conditions. The aim of this study was to explore the relationship between the expression profile of TRPV2 gene and childhood asthma in the north of China. The effects of allergens exposure on the expression of TRPV2 gene were also investigated.
METHODS：Sixty asthmatics children confirmed by physician diagnosis and 60 healthy children as a control group were recruited. Serum total IgE and specific IgE were measured. Using quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR), TRPV2 was detected in total RNA extracted from peripheral blood lymphocytes. Student's t-test and chi-square test were used to analyze the relationship between TRPV2 transcript and different parameter variables on susceptibility of childhood asthma. Multiple logistic regression was used to analyze the associations between TRPV2 gene and allergens.
RESULTS：The expression level of TRPV2 gene was increased 2.6 times in asthmatic children compared with controls (p < .01). The up-regulation of TRPV2 gene and sensitization to one of three the allergens-spring pollen, dust mite, and dog and cat hair-were correlated with childhood asthma. In addition, the hypersensitivity to spring pollen, cockroach, and dust mite and up-regulation of TRPV2 gene expression may be the risk factors for the childhood asthma in Beijing.
CONCLUSIONS：The increased expression of TRPV2 gene in peripheral lymphocytes is closely correlated with childhood asthma in the north of China. This study provides a potential new biomarker of childhood asthma and lays the basis for further clarification of the pathogenesis underlying asthma.

J Asthma. 2013 Jan 7. [Epub ahead of print]