有和无阿司匹林过敏的哮喘患者,支气管阿司匹林激发试验中呼出气类花生酸类物质含量:初步研究
2012/02/29
背景:类花生酸类物质在阿司匹林诱导的哮喘中其特殊作用已经进行过研究。
目的:在哮喘患者中研究阿司匹林对呼出气冷凝液(EBC)中类花生酸类物质含量的影响。
方法:对阿司匹林敏感和阿司匹林耐受的哮喘患者,在基础水平和支气管阿司匹林激发试验中,采用气相色谱/质谱法(GC-MS)和/或高效液相色谱/质谱法(HPLC-MS2)检测EBC中类花生酸类物质含量。
结果:对于阿司匹林敏感患者,阿司匹林能促进支气管反应,但对阿司匹林耐受者无影响(ATAs)。基础水平下,除了脂质氧化产物5-和15-羟基二十碳四烯酸(HETE)在阿司匹林诱导的哮喘患者(AIA)中高于阿司匹林耐受的哮喘患者,其他类花生酸类物质两组间无显著差异。阿司匹林激发试验中,总半胱氨酰-白三烯(cys-LTs)水平在两组患者中维持不变。阿司匹林剂量对阿司匹林过敏哮喘患者呼出气cys-LT和前列腺素类化合物水平有影响。
结论:基础水平下呼出气冷凝液中5-和15-羟基二十碳四烯酸含量较高,可用于检测阿司匹林敏感性哮喘的变化。稳定哮喘患者,支气管阿司匹林激发试验中,呼出气冷凝液中半胱氨酰-白三烯和eoxins不能作为阿司匹林超敏的一个可靠诊断指标。
目的:在哮喘患者中研究阿司匹林对呼出气冷凝液(EBC)中类花生酸类物质含量的影响。
方法:对阿司匹林敏感和阿司匹林耐受的哮喘患者,在基础水平和支气管阿司匹林激发试验中,采用气相色谱/质谱法(GC-MS)和/或高效液相色谱/质谱法(HPLC-MS2)检测EBC中类花生酸类物质含量。
结果:对于阿司匹林敏感患者,阿司匹林能促进支气管反应,但对阿司匹林耐受者无影响(ATAs)。基础水平下,除了脂质氧化产物5-和15-羟基二十碳四烯酸(HETE)在阿司匹林诱导的哮喘患者(AIA)中高于阿司匹林耐受的哮喘患者,其他类花生酸类物质两组间无显著差异。阿司匹林激发试验中,总半胱氨酰-白三烯(cys-LTs)水平在两组患者中维持不变。阿司匹林剂量对阿司匹林过敏哮喘患者呼出气cys-LT和前列腺素类化合物水平有影响。
结论:基础水平下呼出气冷凝液中5-和15-羟基二十碳四烯酸含量较高,可用于检测阿司匹林敏感性哮喘的变化。稳定哮喘患者,支气管阿司匹林激发试验中,呼出气冷凝液中半胱氨酰-白三烯和eoxins不能作为阿司匹林超敏的一个可靠诊断指标。
(苏楠 审校)
J Allergy (Cairo). 2012;2012:696792. Epub 2012 Jan 12.
J Allergy (Cairo). 2012;2012:696792. Epub 2012 Jan 12.
Source
Department of Medicine, Medical College, Jagiellonian University, ul. Skawińska 8, 31-066 Kraków, Poland.
Department of Medicine, Medical College, Jagiellonian University, ul. Skawińska 8, 31-066 Kraków, Poland.
Abstract
BACKGROUND:Special regulatory role of eicosanoids has been postulated in aspirin-induced asthma.
OBJECTIVE:To investigate effects of aspirin on exhaled breath condensate (EBC) levels of eicosanoids in patients with asthma.
METHODS:We determined EBC eicosanoid concentrations using gas chromatography/mass spectrometry (GC-MS) and high-performance liquid chromatography/mass spectrometry (HPLC-MS(2)) or both. Determinations were performed at baseline and following bronchial aspirin challenge, in two well-defined phenotypes of asthma: aspirin-sensitive and aspirin-tolerant patients.
RESULTS:Aspirin precipitated bronchial reactions in all aspirin-sensitive, but in none of aspirin-tolerant patients (ATAs). At baseline, eicosanoids profile did not differ between both asthma groups except for lipoxygenation products: 5- and 15-hydroxyeicosatetraenoic acid (5-, 15-HETE) which were higher in aspirin-induced asthma (AIA) than inaspirin-tolerant subjects. Following aspirin challenge the total levels of cysteinyl-leukotrienes (cys-LTs) remained unchanged in both groups. The dose of aspirin had an effect on magnitude of the response of the exhaled cys-LTs and prostanoids levels only in AIA subjects.
CONCLUSION:The high baseline eicosanoid profiling of lipoxygenation products 5- and 15-HETE in EBC makes it possible to detect alterations in aspirin-sensitive asthma. Cysteinyl-leukotrienes, and eoxins levels in EBC after bronchial aspirin administration in stable asthma patients cannot be used as a reliable diagnostic index for aspirin hypersensitivity.
BACKGROUND:Special regulatory role of eicosanoids has been postulated in aspirin-induced asthma.
OBJECTIVE:To investigate effects of aspirin on exhaled breath condensate (EBC) levels of eicosanoids in patients with asthma.
METHODS:We determined EBC eicosanoid concentrations using gas chromatography/mass spectrometry (GC-MS) and high-performance liquid chromatography/mass spectrometry (HPLC-MS(2)) or both. Determinations were performed at baseline and following bronchial aspirin challenge, in two well-defined phenotypes of asthma: aspirin-sensitive and aspirin-tolerant patients.
RESULTS:Aspirin precipitated bronchial reactions in all aspirin-sensitive, but in none of aspirin-tolerant patients (ATAs). At baseline, eicosanoids profile did not differ between both asthma groups except for lipoxygenation products: 5- and 15-hydroxyeicosatetraenoic acid (5-, 15-HETE) which were higher in aspirin-induced asthma (AIA) than inaspirin-tolerant subjects. Following aspirin challenge the total levels of cysteinyl-leukotrienes (cys-LTs) remained unchanged in both groups. The dose of aspirin had an effect on magnitude of the response of the exhaled cys-LTs and prostanoids levels only in AIA subjects.
CONCLUSION:The high baseline eicosanoid profiling of lipoxygenation products 5- and 15-HETE in EBC makes it possible to detect alterations in aspirin-sensitive asthma. Cysteinyl-leukotrienes, and eoxins levels in EBC after bronchial aspirin administration in stable asthma patients cannot be used as a reliable diagnostic index for aspirin hypersensitivity.
J Allergy (Cairo). 2012;2012:696792. Epub 2012 Jan 12.