诱导痰液中嗜酸性粒细胞的简化检测方法
2011/06/17
摘要
目的与设计：痰液诱导是研究哮喘患者气道炎症的一个安全的无创性方法，但是对样本的分析较为费力，而且需要经过专门培训的技术人员和特殊的实验设备才能完成。本文介绍一种检测诱导痰液（IS）样本中嗜酸性粒细胞炎症的简单方法。
试验方法：对来自81名呼吸道疾病患者的诱导痰液样本进行分析。比较4种不同的方法与欧洲呼吸学会/美国胸科协会推荐的传统方法在检测嗜酸性粒细胞炎症上的差异。
结果：对传统方法进行改良后的新方法，在检测嗜酸性粒细胞炎症中具有简便性，而且对技术要求不高，检测的嗜酸性粒细胞分别为13.0 ± 18.2 vs. 15.4 ± 22.4%（P = 0.19）。
结论：本文介绍的检测嗜酸性粒细胞炎症的简便方法，在不具备采用传统方法检测的实验室内，可作为评价嗜酸性粒细胞炎症的检测手段。
（刘国梁 审校）
Inflamm Res. 2011 Apr 5. [Epub ahead of print]
Simplified detection of eosinophils in induced sputum.
Fireman E, Toledano B, Buchner N, Stark M, Schwarz Y.
Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel, fireman@tasmc.health.gov.il.
Abstract
OBJECTIVE AND DESIGN: The induction of sputum is a safe, noninvasive method of studying airway inflammation in asthma, but the method of analyzing the samples is laborious and requires well-trained technicians using highly specialized laboratory equipment. We introduce a shorter and simpler modification of the process for identifying eosinophilic inflammation from induced sputum (IS) samples.
MATERIAL: Samples of 81 patients referred for IS assessment of respiratory diseases were studied. Four different assessment approaches were studied in comparison with the conventional method of selecting plugs proposed by the European Respiratory Society/American Thoracic Society task force.
RESULTS: One modification of the conventional method of IS processing emerged as simpler to execute and less technologically demanding than the original one (13.0 ± 18.2 vs. 15.4 ± 22.4% eosinophils, P = 0.19).
CONCLUSION: The simpler approach should now encourage the use of IS as a convenient procedure for evaluating eosinophilic inflammation in less sophisticated laboratories.
Inflamm Res. 2011 Apr 5. [Epub ahead of print]
目的与设计：痰液诱导是研究哮喘患者气道炎症的一个安全的无创性方法，但是对样本的分析较为费力，而且需要经过专门培训的技术人员和特殊的实验设备才能完成。本文介绍一种检测诱导痰液（IS）样本中嗜酸性粒细胞炎症的简单方法。
试验方法：对来自81名呼吸道疾病患者的诱导痰液样本进行分析。比较4种不同的方法与欧洲呼吸学会/美国胸科协会推荐的传统方法在检测嗜酸性粒细胞炎症上的差异。
结果：对传统方法进行改良后的新方法，在检测嗜酸性粒细胞炎症中具有简便性，而且对技术要求不高，检测的嗜酸性粒细胞分别为13.0 ± 18.2 vs. 15.4 ± 22.4%（P = 0.19）。
结论：本文介绍的检测嗜酸性粒细胞炎症的简便方法，在不具备采用传统方法检测的实验室内，可作为评价嗜酸性粒细胞炎症的检测手段。
（刘国梁 审校）
Inflamm Res. 2011 Apr 5. [Epub ahead of print]
Simplified detection of eosinophils in induced sputum.
Fireman E, Toledano B, Buchner N, Stark M, Schwarz Y.
Institute of Pulmonary and Allergic Diseases, National Laboratory Service for ILD, Tel-Aviv Sourasky Medical Center, 6 Weizman Street, 64239, Tel-Aviv, Israel, fireman@tasmc.health.gov.il.
Abstract
OBJECTIVE AND DESIGN: The induction of sputum is a safe, noninvasive method of studying airway inflammation in asthma, but the method of analyzing the samples is laborious and requires well-trained technicians using highly specialized laboratory equipment. We introduce a shorter and simpler modification of the process for identifying eosinophilic inflammation from induced sputum (IS) samples.
MATERIAL: Samples of 81 patients referred for IS assessment of respiratory diseases were studied. Four different assessment approaches were studied in comparison with the conventional method of selecting plugs proposed by the European Respiratory Society/American Thoracic Society task force.
RESULTS: One modification of the conventional method of IS processing emerged as simpler to execute and less technologically demanding than the original one (13.0 ± 18.2 vs. 15.4 ± 22.4% eosinophils, P = 0.19).
CONCLUSION: The simpler approach should now encourage the use of IS as a convenient procedure for evaluating eosinophilic inflammation in less sophisticated laboratories.
Inflamm Res. 2011 Apr 5. [Epub ahead of print]
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