咳嗽中传入迷走神经的可塑性
2011/05/12
摘要
在过敏性炎症和病毒性炎症中，气道产生大量神经营养因子。这些因子与感觉神经上的同源受体选择性结合，在炎症性疾病的神经可塑性中起到重要作用。在前期研究中，我们在豚鼠气道中发现了多节的Aδ纤维，在受到刺激时能诱发咳嗽。这些神经纤维不表达TRPV1，因此对辣椒素也不敏感。我们采用单个神经元RT-PCR，对这些多节性Aδ纤维的神经营养因子表达进行了评价。结果显示，这些神经元主要表达TRKB--脑源性神经营养因子(BDNF)和NT4的受体。同时还有GFRα1的表达（胶质细胞源性神经营养因子(GDNF)的受体）。针对气道的BDNF，活化TRKB受体，能导致大部分多节性Aδ神经元表型变化，如这些神经元表达TRPV1。这些结果显示，参与咳嗽的传入神经员的表型特征存在变化，这取决于研究的背景情况。
（陈欣 审校）
Pulm Pharmacol Ther. 2011 Mar 2. [Epub ahead of print]
Neuroplasticity in vagal afferent neurons involved in cough.
Lieu T, Undem BJ.
The Johns Hopkins Medical Institutions, Baltimore, MD, USA.
Abstract
Neurotrophic factors are produced in the airways during allergic and viral inflammation. Their selective interaction with cognate receptors on sensory nerves likely accounts for some of the neuroplasticity that can accompany inflammatory diseases. We have previously described a nodose Aδ fiber in the guinea pig trachea that evokes cough upon stimulation. These nerves do not express TRPV1 and accordingly are capsaicin-insensitive. We evaluated the neurotrophic factor expression in nodose tracheal Aδ fiber neurons using single identified neuron RT-PCR. We found these neuron expressed mainly TRKB; the receptor for brain-derived neurotrophic factor, (BDNF) and NT4. They also expressed GFRα1; the receptor for glial-derived neurotrophic factor (GDNF). Treating the trachea with BDNF, to activate the TRKB receptors, caused a phenotypic change in the vast majority of nodose Aδ neurons such that they expressed TRPV1. These results strengthen the conclusion that the phenotypic characteristics of afferent nerves involved in cough may vary, depending on the context in which they are studied
Pulm Pharmacol Ther. 2011 Mar 2. [Epub ahead of print]
在过敏性炎症和病毒性炎症中，气道产生大量神经营养因子。这些因子与感觉神经上的同源受体选择性结合，在炎症性疾病的神经可塑性中起到重要作用。在前期研究中，我们在豚鼠气道中发现了多节的Aδ纤维，在受到刺激时能诱发咳嗽。这些神经纤维不表达TRPV1，因此对辣椒素也不敏感。我们采用单个神经元RT-PCR，对这些多节性Aδ纤维的神经营养因子表达进行了评价。结果显示，这些神经元主要表达TRKB--脑源性神经营养因子(BDNF)和NT4的受体。同时还有GFRα1的表达（胶质细胞源性神经营养因子(GDNF)的受体）。针对气道的BDNF，活化TRKB受体，能导致大部分多节性Aδ神经元表型变化，如这些神经元表达TRPV1。这些结果显示，参与咳嗽的传入神经员的表型特征存在变化，这取决于研究的背景情况。
（陈欣 审校）
Pulm Pharmacol Ther. 2011 Mar 2. [Epub ahead of print]
Neuroplasticity in vagal afferent neurons involved in cough.
Lieu T, Undem BJ.
The Johns Hopkins Medical Institutions, Baltimore, MD, USA.
Abstract
Neurotrophic factors are produced in the airways during allergic and viral inflammation. Their selective interaction with cognate receptors on sensory nerves likely accounts for some of the neuroplasticity that can accompany inflammatory diseases. We have previously described a nodose Aδ fiber in the guinea pig trachea that evokes cough upon stimulation. These nerves do not express TRPV1 and accordingly are capsaicin-insensitive. We evaluated the neurotrophic factor expression in nodose tracheal Aδ fiber neurons using single identified neuron RT-PCR. We found these neuron expressed mainly TRKB; the receptor for brain-derived neurotrophic factor, (BDNF) and NT4. They also expressed GFRα1; the receptor for glial-derived neurotrophic factor (GDNF). Treating the trachea with BDNF, to activate the TRKB receptors, caused a phenotypic change in the vast majority of nodose Aδ neurons such that they expressed TRPV1. These results strengthen the conclusion that the phenotypic characteristics of afferent nerves involved in cough may vary, depending on the context in which they are studied
Pulm Pharmacol Ther. 2011 Mar 2. [Epub ahead of print]
