血浆脑源性神经营养因子水平与学龄期哮喘患儿临床严重程度相关
2010/12/31
摘要
背景：哮喘表现为气道的慢性炎症，具有明显的白细胞浸润、细胞活化和组织重构改变。脑源性神经营养因子（BDNF）已经被证实参与了哮喘和过敏性疾病的致病过程，但将其作为儿童哮喘严重程度的一个标志物尚未进行临床研究。
目的：在持续哮喘获得控制的患儿（6～15岁）中评价血浆BDNF和炎症标志物与疾病严重程度之间的关系。
方法：患有持续性哮喘的患儿入选本研究，对所有患者进行肺功能检测和皮肤针刺试验。采用ELISA检测血浆BDNF水平和多个炎症标记物(CCL3, CCL11, CCL22, CCL24, CXCL8, CXCL9, CXCL10, 可溶性TNF 受体)。
结果：与轻度哮喘患儿和哮喘控制的患儿相比，中度和重度哮喘患儿具有较高的血浆BDNF水平(P<0.001)。研究的趋化因子和可溶性TNF受体在各组之间未见显著差异。
结论和临床相关性：我们的结果显示，BDNF是临床评价儿童哮喘严重程度的一个潜在生物标记物。
（陈欣 审校）
Clin Exp Allergy. 2010 Dec;40(12):1755-9. doi:10.1111/j.1365-2222.2010.03618.x.
Plasma brain-derived neurotrophic factor levels are associated with clinical severity in school age children with asthma.
Müller GC, Pitrez PM, Teixeira AL, Pires PS, Jones MH, Stein RT, Bauer ME.
Laboratory of Cellular and Molecular Immunology Laboratory of Pediatric Pulmonology, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil Department of Internal Medicine, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Abstract
Background Asthma is characterized by chronic inflammation of the airways with significant changes in leucocyte trafficking, cellular activation and tissue remodelling. Brain-derived neurotrophic factor (BDNF) has been involved with asthma and allergic diseases but its role as a severity marker in paediatric asthma has not been clinically assessed. Objectives To evaluate plasma BDNF and inflammatory markers in order to address their relationships with disease severity in children (6-15 years) with controlled persistent asthma. Methods Children with persistent asthma were selected and lung function and skin prick tests were performed in all patients. Plasma BDNF levels and various inflammatory markers (CCL3, CCL11, CCL22, CCL24, CXCL8, CXCL9, CXCL10, soluble TNF receptors) were assessed by ELISAs. Results Subjects with moderate and severe asthma had higher BDNF levels than mild asthma and controls (P<0.001). The chemokines studied and soluble TNF receptors did not differ between the studied groups. Conclusion and Clinical Relevance Our results indicate BDNF as a potential biomarker for clinical severity in children with asthma.
Clin Exp Allergy. 2010 Dec;40(12):1755-9. doi: 10.1111/j.1365-2222.2010.03618.x.
背景：哮喘表现为气道的慢性炎症，具有明显的白细胞浸润、细胞活化和组织重构改变。脑源性神经营养因子（BDNF）已经被证实参与了哮喘和过敏性疾病的致病过程，但将其作为儿童哮喘严重程度的一个标志物尚未进行临床研究。
目的：在持续哮喘获得控制的患儿（6～15岁）中评价血浆BDNF和炎症标志物与疾病严重程度之间的关系。
方法：患有持续性哮喘的患儿入选本研究，对所有患者进行肺功能检测和皮肤针刺试验。采用ELISA检测血浆BDNF水平和多个炎症标记物(CCL3, CCL11, CCL22, CCL24, CXCL8, CXCL9, CXCL10, 可溶性TNF 受体)。
结果：与轻度哮喘患儿和哮喘控制的患儿相比，中度和重度哮喘患儿具有较高的血浆BDNF水平(P<0.001)。研究的趋化因子和可溶性TNF受体在各组之间未见显著差异。
结论和临床相关性：我们的结果显示，BDNF是临床评价儿童哮喘严重程度的一个潜在生物标记物。
（陈欣 审校）
Clin Exp Allergy. 2010 Dec;40(12):1755-9. doi:10.1111/j.1365-2222.2010.03618.x.
Plasma brain-derived neurotrophic factor levels are associated with clinical severity in school age children with asthma.
Müller GC, Pitrez PM, Teixeira AL, Pires PS, Jones MH, Stein RT, Bauer ME.
Laboratory of Cellular and Molecular Immunology Laboratory of Pediatric Pulmonology, Instituto de Pesquisas Biomédicas, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil Department of Internal Medicine, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Abstract
Background Asthma is characterized by chronic inflammation of the airways with significant changes in leucocyte trafficking, cellular activation and tissue remodelling. Brain-derived neurotrophic factor (BDNF) has been involved with asthma and allergic diseases but its role as a severity marker in paediatric asthma has not been clinically assessed. Objectives To evaluate plasma BDNF and inflammatory markers in order to address their relationships with disease severity in children (6-15 years) with controlled persistent asthma. Methods Children with persistent asthma were selected and lung function and skin prick tests were performed in all patients. Plasma BDNF levels and various inflammatory markers (CCL3, CCL11, CCL22, CCL24, CXCL8, CXCL9, CXCL10, soluble TNF receptors) were assessed by ELISAs. Results Subjects with moderate and severe asthma had higher BDNF levels than mild asthma and controls (P<0.001). The chemokines studied and soluble TNF receptors did not differ between the studied groups. Conclusion and Clinical Relevance Our results indicate BDNF as a potential biomarker for clinical severity in children with asthma.
Clin Exp Allergy. 2010 Dec;40(12):1755-9. doi: 10.1111/j.1365-2222.2010.03618.x.
