哮喘患者红细胞膜鞘磷脂代谢
2010/12/29
摘要
含有胆固醇的鞘磷脂（SM）是胞浆膜外层的主要组成部分，由多个微结构域组成，后者又称之为脂质筏。这些脂质筏为蛋白、受体、酶及其他组成部分提供了支撑，并组织和引导这些组成部分完成细胞功能，包括由细胞外物质引起的跨膜信号传递。SM的成分受到其代谢的影响。SM组成的变化也会影响脂质筏的组成和细胞对哮喘触发物产生的反应，而后者进一步导致病理生理学变化。细胞膜的变化的研究中，含有脂质筏的红细胞是最佳的研究对象。本试验旨在研究哮喘患者的红细胞胞浆膜。
目的：旨在了解哮喘患者SM代谢变化。
方法：本研究入选50名对象（25名哮喘患者和25名健康受试者）。分离外周血红细胞，提取红细胞胞膜。检测总胆固醇、磷脂、SM和鞘磷脂酶活性。P<0.05为统计学显著性。
结果与结论：对于哮喘患者，胆固醇含量显著降低（P<0.05）、总磷脂含量下降（P<0.005）、SM增加（P<0.01）、胆固醇:SM比值下降（P<0.001）及鞘磷脂酶活性增加（P<0.001）。我们认为，哮喘患者SM含量增加与鞘磷脂酶活性增加相关，表明SM代谢存在失衡，朝着SM积聚的方向发展。胆固醇与SM比值是维持脂质筏的一个重要指标，哮喘患者该比值也显著下降，这表明患者的脂质筏结构存在改变，从而导致病理生理学变化，最终发展为哮喘。SM代谢的调节有助于哮喘的调节与控制。
（苏楠 审校）
J Asthma.2010 Nov;47(9):966-971.
Sphingomyelin metabolism in erythrocyte membrane in asthma.
Gupta P, Vijayan VK, Bansal SK.
Department of Biochemistry, V. P. Chest Institute, University of Delhi, Delhi, India.
Abstract
Background. Sphingomyelin (SM), a major lipid constituent of outer leaflet of plasma membranes, with cholesterol, constitutes microdomains, which are termed as lipid rafts. These rafts provide support to proteins, receptors, enzymes, and so on and organize and orient them to conduct cellular functions including transmembrane signaling to substances in external milieu. The SM contents are regulated by its metabolism, changes in which may affect the composition of lipid rafts and cell response to the triggers of asthma which may lead to the pathophysiology. For studying changes in membranes, erythrocytes, which contain lipid rafts, are considered to be the best cell type. Hence, this study was conducted on plasma membrane of erythrocytes of asthmatic patients.
Objective. The objective is to understand the changes in SM metabolism in asthma. Methods. The study included 50 subjects (25 asthmatics and 25 healthy subjects). Erythrocytes were isolated from the peripheral blood and membrane prepared. This was followed by determination of total cholesterol, phospholipids, SM, and sphingomyelinase activity. P < .05 was considered significant. Results and conclusions In asthmatics, there was a significant decrease in cholesterol contents (p < .05), decrease in total phospholipid contents (p < .005), increase in SM (p < .01), decrease in cholesterol: SM ratio (p < .001) and increase in sphingomyelinase activity (p < .001) in erythrocyte membranes. We conclude that in asthma, the increase in SM contents is associated with increased sphingomyelinase activity which shows an imbalance in SM metabolism, directed toward its accumulation. The ratio of cholesterol to SM, critical for maintenance of lipid rafts, was significantly lower in asthmatics. This indicates changes in structure of lipid rafts which may lead to the pathophysiology and development of asthma. Regulation of SM metabolism may help in disease regulation and its control.
J Asthma. 2010 Nov;47(9):966-71.
含有胆固醇的鞘磷脂（SM）是胞浆膜外层的主要组成部分，由多个微结构域组成，后者又称之为脂质筏。这些脂质筏为蛋白、受体、酶及其他组成部分提供了支撑，并组织和引导这些组成部分完成细胞功能，包括由细胞外物质引起的跨膜信号传递。SM的成分受到其代谢的影响。SM组成的变化也会影响脂质筏的组成和细胞对哮喘触发物产生的反应，而后者进一步导致病理生理学变化。细胞膜的变化的研究中，含有脂质筏的红细胞是最佳的研究对象。本试验旨在研究哮喘患者的红细胞胞浆膜。
目的：旨在了解哮喘患者SM代谢变化。
方法：本研究入选50名对象（25名哮喘患者和25名健康受试者）。分离外周血红细胞，提取红细胞胞膜。检测总胆固醇、磷脂、SM和鞘磷脂酶活性。P<0.05为统计学显著性。
结果与结论：对于哮喘患者，胆固醇含量显著降低（P<0.05）、总磷脂含量下降（P<0.005）、SM增加（P<0.01）、胆固醇:SM比值下降（P<0.001）及鞘磷脂酶活性增加（P<0.001）。我们认为，哮喘患者SM含量增加与鞘磷脂酶活性增加相关，表明SM代谢存在失衡，朝着SM积聚的方向发展。胆固醇与SM比值是维持脂质筏的一个重要指标，哮喘患者该比值也显著下降，这表明患者的脂质筏结构存在改变，从而导致病理生理学变化，最终发展为哮喘。SM代谢的调节有助于哮喘的调节与控制。
（苏楠 审校）
J Asthma.2010 Nov;47(9):966-971.
Sphingomyelin metabolism in erythrocyte membrane in asthma.
Gupta P, Vijayan VK, Bansal SK.
Department of Biochemistry, V. P. Chest Institute, University of Delhi, Delhi, India.
Abstract
Background. Sphingomyelin (SM), a major lipid constituent of outer leaflet of plasma membranes, with cholesterol, constitutes microdomains, which are termed as lipid rafts. These rafts provide support to proteins, receptors, enzymes, and so on and organize and orient them to conduct cellular functions including transmembrane signaling to substances in external milieu. The SM contents are regulated by its metabolism, changes in which may affect the composition of lipid rafts and cell response to the triggers of asthma which may lead to the pathophysiology. For studying changes in membranes, erythrocytes, which contain lipid rafts, are considered to be the best cell type. Hence, this study was conducted on plasma membrane of erythrocytes of asthmatic patients.
Objective. The objective is to understand the changes in SM metabolism in asthma. Methods. The study included 50 subjects (25 asthmatics and 25 healthy subjects). Erythrocytes were isolated from the peripheral blood and membrane prepared. This was followed by determination of total cholesterol, phospholipids, SM, and sphingomyelinase activity. P < .05 was considered significant. Results and conclusions In asthmatics, there was a significant decrease in cholesterol contents (p < .05), decrease in total phospholipid contents (p < .005), increase in SM (p < .01), decrease in cholesterol: SM ratio (p < .001) and increase in sphingomyelinase activity (p < .001) in erythrocyte membranes. We conclude that in asthma, the increase in SM contents is associated with increased sphingomyelinase activity which shows an imbalance in SM metabolism, directed toward its accumulation. The ratio of cholesterol to SM, critical for maintenance of lipid rafts, was significantly lower in asthmatics. This indicates changes in structure of lipid rafts which may lead to the pathophysiology and development of asthma. Regulation of SM metabolism may help in disease regulation and its control.
J Asthma. 2010 Nov;47(9):966-71.
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