趋化因子（C-X-C 基序）配体12/基质细胞衍生因子-1（SDF-1）与哮喘白细胞募集相关
2010/12/07
背景：涉及大量白细胞浸润的过敏性气道炎症是哮喘的特征。基质细胞衍生因子-1（stromal cell-derived factor, SDF-1）或者趋化因子配体12(C-X-C motif ligand 12, CXCL12)可募集大量的细胞，包括静止T淋巴细胞、单核细胞、CD34+干细胞、嗜碱粒细胞和成熟嗜酸粒细胞。然而，据我们了解到目前为止，CXCL12/SDF-1与哮喘白细胞募集相关的潜在作用尚未得到证实。
方法：通过酶联免疫吸附法（ELISA）测定15例哮喘患者和13例健康受试者支气管肺泡灌洗液(BALF)中CXCL12/SDF-1的水平。采用免疫组化方法确定趋化因子的细胞来源。
结果：与健康对照组比较，哮喘患者BALF中CXCL12/SDF-1 浓度显著升高(Med=845 pg/mL，Range=[296, 1,700] pg/mL vs Med=55 pg/mL，Range=[25,, 97] pg/mL; P < .001)。CXCL12/SDF-1的浓度与巨噬细胞(r = 0.728, P < .01)、淋巴细胞(r = 0.728, P < .01)、嗜酸粒细胞(r = 0.765, P < .01)有关。通过免疫组织化学方法发现，CXCL12/SDF-1表达于气道上皮细胞，在较小范围可表达于单核细胞。
结论：在哮喘患者BALF中，CXCL12/SDF-1呈高浓度释放。BALF趋化因子的浓度和白细胞数量相关的发现表明，该趋化因子可促成哮喘白细胞的募集。
（王刚 四川大学华西医院中西医结合科 610041 摘译 ）
（Chest. 2010 ; 138 (1):100-106）
Chemokine (C-X-C Motif) Ligand 12/Stromal Cell-Derived Factor-1 is associated with leukocyte recruitment in asthma
Negrete-García M.C, Velazquez JR, Popoca-Coyotl A, Montes-Vizuet AR, Juárez-Carvajal EJ,Teran LM.
Chest. 2010 ; 138 (1):100-106
Background: Asthma is characterized by allergic airway infl ammatory response involving extensive leukocyte infi ltration. The stromal cell-derived factor (SDF)-1 or chemokine (C-X-C motif) ligand 12 (CXCL12) attracts a number of cells, including resting T lymphocytes, monocytes, CD34+ stem cells, basophils, and mature eosinophils. To date, however, the potential role of CXCL12/SDF-1 in relation to leukocyte recruitment in asthma has not been previously examined, to our knowledge.
Methods: Levels of CXCL12/SDF-1 in the BAL fl uid (BALF) of 15 subjects with asthma and 13 healthy subjects were measured by enzyme-linked immunosorbent assay. Immunohistochemistry was performed to identify the cellular source of this chemokine.
Results: CXCL12/SDF-1 concentrations were signifi cantly elevated in BALF from subjects with asthma compared with normal subjects (median 845 pg/mL, range, 296-1,700 pg/mL vs median 55 pg/mL, range 25-97 pg/mL; P < .001). Concentrations of CXCL12/SDF-1 correlated with macrophages ( r 5 0.728, P < .01), lymphocytes ( r 5 0.651, P < .01), and eosinophils ( r 5 0.765, P < .01). By immunohistochemistry, CXCL12/SDF-1 was localized to the airway epithelium and to a lesser extent to mononuclear cells.
Conclusion: CXCL12/SDF-1 is released in high concentration in BALF of patients with asthma. The finding that concentrations of this chemokine correlated with leukocyte numbers in BALF suggests that this chemokine may contribute to the cell recruitment in asthma.
上一篇：
成人中重度哮喘患者支气管对组胺的反应性与气道重构有关
下一篇：
拮抗TIM-1可以阻断人源化变应性哮喘小鼠模型的疾病进展
