有氧训练逆转哮喘模型小鼠的气道炎症和气道重构
2010/08/20
关键词：有氧训练；气道炎症；气道重构；哮喘；运动；康复
有氧训练（Aerobic training， AT）可以减少呼吸困难和运动诱导的气道痉挛，并改善有氧代谢能力和生活质量。然而，AT这些作用的机制尚不明确。
为了评价AT对哮喘小鼠慢性气道炎症和重构的作用，Silva等将试验小鼠分为对照组、AT组、OVA组、OVA+AT组。AT组小鼠自第28天开始为期4周的有氧训练，每周5次，每次60分钟。测定呼吸力、特异IgE和IgG、胶原沉积和弹力蛋白沉积、平滑肌厚度、上皮细胞粘液、支气管周围嗜酸性粒细胞密度，以及CD3+ 、CD4+、IL-4、IL-5、IL-13、IL- 、IL-2、IL-1ra、IL-10、NF- B和Foxp3等
OVA组显示IgE和IgG1,增多，嗜酸性粒细胞增多，CD3+, CD4+, IL-4, IL-5, IL-13, NF- B水平升高、胶原蛋白、弹性蛋白和粘液合成增多、平滑肌增厚以及肺组织阻力和弹性增加。在OVA+AT组虽然IgE and IgG1增多，但嗜酸性粒细胞、CD3+, CD4+, IL-4, IL-5, IL-13, NF- B减少，气道重构、粘液合成、平滑肌增厚和肺组织抵抗和弹性较OVA组减轻 (p<0.05)。同时OVA+AT组小鼠的 IL-10和IL-1ra高于OVA组(p<0.05)，但Foxp3无显著性差异。
本研究发现AT能减轻气道炎症、气道重建以及TH2型反应并能改善呼吸力学。这些作用似乎是与IL-10和IL-1ra增多以及NF- B下降有关。
（韩伟 青岛大学附属青岛市立医院东院 266071 摘译）
(Eur Respir J 2010; 35:994-1002)
(Eur Respir J 2010; 35:994-1002)
Aerobic training reverses airway inflammation and remodelling in an asthma murine model
R. A. Silva1, R. P. Vieira1, A. C. S. Duarte1, F. D. T. Q. S. Lopes2, A. Perini2, T. Mauad3, M. A. Martins2 and C. R. F. Carvalho1
Keywords: Aerobic training, airway inflammation, airway remodelling, asthma, exercise, rehabilitation
Aerobic training (AT) decreases dyspnoea and exercise-induced bronchospasm, and improves aerobic capacity and quality of life; however, the mechanisms for such benefits remain poorly understood. The aim of the present study was to evaluate the AT effects in a chronic model of allergic lung inflammation in mice after the establishment of airway inflammation and remodelling.
Mice were divided into the control group, AT group, ovalbumin (OVA) group or OVA+AT group and exposed to saline or OVA. AT was started on day 28 for 60 min five times per week for 4 weeks. Respiratory mechanics, specific immunoglobulin (Ig)E and IgG1, collagen and elastic fibres deposition, smooth muscle thickness, epithelial mucus, and peribronchial density of eosinophils, CD3+ and CD4+, IL-4, IL-5, IL-13, interferon- , IL-2, IL-1ra, IL-10, nuclear factor (NF)- B and Foxp3 were evaluated.
The OVA group showed an increase in IgE and IgG1, eosinophils, CD3+, CD4+, IL-4, IL-5, IL-13, NF- B, collagen and elastic, mucus synthesis, smooth muscle thickness and lung tissue resistance and elastance. The OVA+AT group demonstrated an increase of IgE and IgG1, and reduction of eosinophils, CD3+, CD4+, IL-4, IL-5, IL-13, NF- B, airway remodelling, mucus synthesis, smooth muscle thickness and tissue resistance and elastance compared with the OVA group (p<0.05). The OVA+AT group also showed an increase in IL-10 and IL-1ra (p<0.05), independently of Foxp3.
AT reversed airway inflammation and remodelling and T-helper cell 2 response, and improved respiratory mechanics. These results seem to occur due to an increase in the expression of IL-10 and IL-1ra and a decrease of NF- B.
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OX40/OX40L及IL-4在哮喘动物模型气道壁的表达
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不同哮喘表型中性粒细胞基因表达和细胞因子合成的差异
