哮喘患者的CCR8表达增加
2010/08/11
背景：趋化因子及其受体在T细胞向哮喘患者肺部聚集过程中起到了重要作用。CCR8特异性表达于Th2细胞中，在人体哮喘的发病过程中起到重要作用。
目的：本研究旨在哮喘和健康人中检测血液、支气管肺泡灌洗液（BAL）和支气管粘膜内T细胞的CCR8的表达。
方法：CCR8在血液和BAL的表达采用流式细胞仪检测。采用佛波醇-肉豆蔻-醋酸和钙离子载体刺激后，检测血液和BAL中 IFN-γ(+)和IL-4(+)/IL-13(+)T细胞中CCR8的表达。支气管活检标本石蜡包埋，检测支气管上皮CCR8的表达。结果：表达CCR8的CD3(+) 阳性细胞比率，哮喘患者（4.7+/-0.4%）高于正常个体（3.0+/-0.4%; P<0.01）。在所有研究对象中，血液和BAL中，IL-4(+)/IL-13(+)T细胞的CCR8表达是IFN-γ(+)T细胞的6倍。与正常个体（3.9+/-0.7%）相比，哮喘患者BAL中更多的T细胞（8.6+/-0.8%）表达CCR8（P<0.01）。同一哮喘患者的血液和BAL中，BAL中的CCR8+CD3(+) T细胞（9.0+/-0.9%）较血液显著增加（5.6+/-0.9%; P<0.05）。此外，哮喘患者支气管活检标本中CCR8阳性细胞（93+/-11个/mm2）显著高于正常个体（30+/-16个/mm2）（P<0.05）。同时，哮喘患者BAL中配体CCL1较正常个体显著增加（35+/-6 vs. 12.9+/-7 pg/mL；P<0.05）。
结论：CCR8在趋化T细胞至哮喘患者肺部过程中起到重要作用。
（苏楠 审校）
Clin Exp Allergy. 2010 Apr 28. [Epub ahead of print]
Expression of CCR8 is increased in asthma.
Mutalithas K, Guillen C, Raport C, Kolbeck R, Soler D, Brightling CE, Pavord ID, Wardlaw AJ.
Department of Infection, Immunity and Inflammation, Institute for Lung Health, University of Leicester, Leicester, UK.
Abstract
Summary Background Chemokines and their receptors could play key roles in the recruitment of T cells to the asthmatic lung. CCR8 is preferentially expressed on T-helper type 2 cells, and is thought to play a role in the pathogenesis of human asthma. Objective Determine the expression of CCR8 on T cells in blood, bronchoalveolar lavage (BAL) and bronchial mucosa from asthmatics and normal subjects. Methods CCR8 expression in blood and BAL from asthma and normal subjects was studied using flow cytometry. CCR8 expression on IFN-gamma(+) and IL-4(+)/IL-13(+) blood and BAL T cells was studied following stimulation with Phorbol-Myristate-Acetate and Calcium Ionophore. Paraffin-embedded bronchial biopsies were used to study CCR8 in bronchial epithelium. Results The percentage of CD3(+) cells expressing CCR8 in the blood was higher in asthmatics (4.7+/-0.4%) compared with normal subjects (3.0+/-0.4%; P<0.01). There was an approximately sixfold enrichment of CCR8 on IL-4(+)/IL-13(+) cells compared with IFN-gamma(+) T cells (P<0.001) in both asthmatic and normal subjects in both blood and BAL. Significantly more BAL T cells expressed CCR8 in asthmatic (8.6+/-0.8%) compared with normal subjects (3.9+/-0.7%) (P<0.01). In paired blood-BAL samples from asthmatics, significantly more CCR8+CD3(+) T cells were present in BAL (9.0+/-0.9%) than in blood (5.6+/-0.9%; P<0.05). There were more CCR8-positive cells in bronchial biopsies from asthmatic (93+/-11 cells/mm(2)) compared with normal subjects (30+/-16 cells/mm(2)) (P<0.05). The ligand CCL1 was increased in the BAL of asthmatics compared with normal subjects (35+/-6 vs. 12.9+/-7 pg/mL; P<0.05). Conclusion There may be a role for CCR8 in the recruitment of T cells to the lung in asthmatics.
Clin Exp Allergy. 2010 Apr 28. [Epub ahead of print]
目的：本研究旨在哮喘和健康人中检测血液、支气管肺泡灌洗液（BAL）和支气管粘膜内T细胞的CCR8的表达。
方法：CCR8在血液和BAL的表达采用流式细胞仪检测。采用佛波醇-肉豆蔻-醋酸和钙离子载体刺激后，检测血液和BAL中 IFN-γ(+)和IL-4(+)/IL-13(+)T细胞中CCR8的表达。支气管活检标本石蜡包埋，检测支气管上皮CCR8的表达。结果：表达CCR8的CD3(+) 阳性细胞比率，哮喘患者（4.7+/-0.4%）高于正常个体（3.0+/-0.4%; P<0.01）。在所有研究对象中，血液和BAL中，IL-4(+)/IL-13(+)T细胞的CCR8表达是IFN-γ(+)T细胞的6倍。与正常个体（3.9+/-0.7%）相比，哮喘患者BAL中更多的T细胞（8.6+/-0.8%）表达CCR8（P<0.01）。同一哮喘患者的血液和BAL中，BAL中的CCR8+CD3(+) T细胞（9.0+/-0.9%）较血液显著增加（5.6+/-0.9%; P<0.05）。此外，哮喘患者支气管活检标本中CCR8阳性细胞（93+/-11个/mm2）显著高于正常个体（30+/-16个/mm2）（P<0.05）。同时，哮喘患者BAL中配体CCL1较正常个体显著增加（35+/-6 vs. 12.9+/-7 pg/mL；P<0.05）。
结论：CCR8在趋化T细胞至哮喘患者肺部过程中起到重要作用。
（苏楠 审校）
Clin Exp Allergy. 2010 Apr 28. [Epub ahead of print]
Expression of CCR8 is increased in asthma.
Mutalithas K, Guillen C, Raport C, Kolbeck R, Soler D, Brightling CE, Pavord ID, Wardlaw AJ.
Department of Infection, Immunity and Inflammation, Institute for Lung Health, University of Leicester, Leicester, UK.
Abstract
Summary Background Chemokines and their receptors could play key roles in the recruitment of T cells to the asthmatic lung. CCR8 is preferentially expressed on T-helper type 2 cells, and is thought to play a role in the pathogenesis of human asthma. Objective Determine the expression of CCR8 on T cells in blood, bronchoalveolar lavage (BAL) and bronchial mucosa from asthmatics and normal subjects. Methods CCR8 expression in blood and BAL from asthma and normal subjects was studied using flow cytometry. CCR8 expression on IFN-gamma(+) and IL-4(+)/IL-13(+) blood and BAL T cells was studied following stimulation with Phorbol-Myristate-Acetate and Calcium Ionophore. Paraffin-embedded bronchial biopsies were used to study CCR8 in bronchial epithelium. Results The percentage of CD3(+) cells expressing CCR8 in the blood was higher in asthmatics (4.7+/-0.4%) compared with normal subjects (3.0+/-0.4%; P<0.01). There was an approximately sixfold enrichment of CCR8 on IL-4(+)/IL-13(+) cells compared with IFN-gamma(+) T cells (P<0.001) in both asthmatic and normal subjects in both blood and BAL. Significantly more BAL T cells expressed CCR8 in asthmatic (8.6+/-0.8%) compared with normal subjects (3.9+/-0.7%) (P<0.01). In paired blood-BAL samples from asthmatics, significantly more CCR8+CD3(+) T cells were present in BAL (9.0+/-0.9%) than in blood (5.6+/-0.9%; P<0.05). There were more CCR8-positive cells in bronchial biopsies from asthmatic (93+/-11 cells/mm(2)) compared with normal subjects (30+/-16 cells/mm(2)) (P<0.05). The ligand CCL1 was increased in the BAL of asthmatics compared with normal subjects (35+/-6 vs. 12.9+/-7 pg/mL; P<0.05). Conclusion There may be a role for CCR8 in the recruitment of T cells to the lung in asthmatics.
Clin Exp Allergy. 2010 Apr 28. [Epub ahead of print]
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