采用酶免疫分析和气相色谱-质谱分析可疑哮喘患儿呼出气冷凝液中8-异前列腺素含量
2010/02/23
哮喘患者气道主要表现为氧化应激增加，后者可通过检测生物标记物（如8-异前列腺素）来证实。本实验旨在研究1）可疑哮喘及哮喘控制较好的患儿中检测呼出气冷凝液（EBC）及尿液中8-异前列腺素含量；2）比较气相色谱/负离子化学电离质谱（GC/NICI-MS）及酶免疫分析（EIA）在检测8-异前列腺素浓度上的差异。
纳入20例过敏性哮喘患儿，其中13例患儿哮喘控制较好，7例为可疑哮喘患儿。入选者接受呼出气一氧化氮检测和肺活量检测，收集EBC和尿液。分别采用GC/NICI-MS和EIA检测EBC中的8-异前列腺素含量。
与哮喘控制较好患儿相比，可疑哮喘患儿EBC内8-异前列腺素含量显著增加（P＝0.01）。GC/NICI-MS和EIA在检测8-异前列腺素中结果的重复性较好（重复性系数11.5 pg/ml）。尿液8-异前列腺素含量与EBC8-异前列腺素含量无相关性。
可疑哮喘患儿EBC中8-异前列腺素含量显著增加，这表明氧化应激参与了该型哮喘的致病。此外，虽然GC/NICI-MS检测具有更高的准确性，EIA相比GC/NICI-MS在检测8-异前列腺素含量具有良好的可重复性。
（刘国梁 审校）
Eur Respir J. 2009 Nov 6. [Epub ahead of print]
EIA and GC-MS analysis of 8-isoprostane in EBC of children with problematic asthma.
Carraro S, Cogo PE, Isak I, Simonato M, Corradi M, Carnielli VP, Baraldi E.
Asthmatic airways are characterized by enhanced oxidative stress, which can be studied by measuring biomarkers, such as 8-isoprostane. Aims of the present study were: 1) to measure the concentrations of 8-isoprostane in exhaled breath condensate (EBC) and urine of children with problematic and well-controlled asthma; 2) to compare the concentrations of 8-isoprostane measured by gas chromatographic/negative ion chemical ionization mass spectrometry (GC/NICI-MS) and by an enzymatic immunoassay (EIA).We recruited 20 asthmatic allergic children, 13 with well-controlled asthma and 7 with problematic asthma. They underwent exhaled nitric oxide measurements and spirometry, and both EBC and urine samples were collected. 8-isoprostane was measured in EBC by GC/NICI-MS and EIA.8-isoprostane concentrations in EBC were significantly higher in children with problematic asthma than in children with well-controlled asthma (p=0.01). An acceptable reproducibility emerged between GC/NICI-MS and EIA (coefficient of reproducibility 11.5 pg.mL(-1)). 8-isoprostane levels measured in urine did not correlate with those measured in EBC.We showed that 8-isoprostane in EBC was significantly increased in children with problematic asthma, suggesting a role for oxidative stress in this asthma phenotype. In addition we found an acceptable reproducibility of EIA compared to GC/NICI-MS, even if the latter method had higher accuracy.
Carraro S, Cogo PE, Isak I, et al. Eur Respir J. 2009 Nov 6. [Epub ahead of print]
纳入20例过敏性哮喘患儿，其中13例患儿哮喘控制较好，7例为可疑哮喘患儿。入选者接受呼出气一氧化氮检测和肺活量检测，收集EBC和尿液。分别采用GC/NICI-MS和EIA检测EBC中的8-异前列腺素含量。
与哮喘控制较好患儿相比，可疑哮喘患儿EBC内8-异前列腺素含量显著增加（P＝0.01）。GC/NICI-MS和EIA在检测8-异前列腺素中结果的重复性较好（重复性系数11.5 pg/ml）。尿液8-异前列腺素含量与EBC8-异前列腺素含量无相关性。
可疑哮喘患儿EBC中8-异前列腺素含量显著增加，这表明氧化应激参与了该型哮喘的致病。此外，虽然GC/NICI-MS检测具有更高的准确性，EIA相比GC/NICI-MS在检测8-异前列腺素含量具有良好的可重复性。
（刘国梁 审校）
Eur Respir J. 2009 Nov 6. [Epub ahead of print]
EIA and GC-MS analysis of 8-isoprostane in EBC of children with problematic asthma.
Carraro S, Cogo PE, Isak I, Simonato M, Corradi M, Carnielli VP, Baraldi E.
Asthmatic airways are characterized by enhanced oxidative stress, which can be studied by measuring biomarkers, such as 8-isoprostane. Aims of the present study were: 1) to measure the concentrations of 8-isoprostane in exhaled breath condensate (EBC) and urine of children with problematic and well-controlled asthma; 2) to compare the concentrations of 8-isoprostane measured by gas chromatographic/negative ion chemical ionization mass spectrometry (GC/NICI-MS) and by an enzymatic immunoassay (EIA).We recruited 20 asthmatic allergic children, 13 with well-controlled asthma and 7 with problematic asthma. They underwent exhaled nitric oxide measurements and spirometry, and both EBC and urine samples were collected. 8-isoprostane was measured in EBC by GC/NICI-MS and EIA.8-isoprostane concentrations in EBC were significantly higher in children with problematic asthma than in children with well-controlled asthma (p=0.01). An acceptable reproducibility emerged between GC/NICI-MS and EIA (coefficient of reproducibility 11.5 pg.mL(-1)). 8-isoprostane levels measured in urine did not correlate with those measured in EBC.We showed that 8-isoprostane in EBC was significantly increased in children with problematic asthma, suggesting a role for oxidative stress in this asthma phenotype. In addition we found an acceptable reproducibility of EIA compared to GC/NICI-MS, even if the latter method had higher accuracy.
Carraro S, Cogo PE, Isak I, et al. Eur Respir J. 2009 Nov 6. [Epub ahead of print]
上一篇：
风团长径是评价皮肤点刺试验的最佳检测指标
下一篇：
乙酰甲胆碱激发试验的季节性变化
