屋尘螨抗原诱发的慢性哮喘小鼠模型中曲尼司特和己酮可可碱的有效性
2010/02/23
由于曲尼司特对肥大细胞具有抑制作用，因此被用于过敏性疾病的治疗，并且在某些疾病中显示出抗纤维化作用。己酮可可碱（PTX）为甲基黄嘌呤衍生物，能抑制Th1细胞因子释放而具有较强的抗炎作用，但对于Th2细胞因子的作用尚不清楚。
本实验用7周龄雌性BALB/c小鼠建立慢性哮喘模型。小鼠连续7周采用屋尘螨（HDM）抗原致敏。致敏前，每组小鼠腹腔分别注射曲尼司特、PTX或联合注射曲尼司特与PTX。小鼠慢性哮喘模型中，曲尼司特和PTX均能抑制气道重构、减轻气道高反应性（AHR）和气道炎症。上述保护作用与对Th2细胞因子IL-13和TGF-β1抑制相关。
免疫组织化学分析结果显示，各组支气管周围三色染色区域下降与支气管周围平滑肌细胞增生、1型和3型胶原沉积改善相关。上述药物对慢性哮喘，尤其是气道重构方面具有潜在治疗作用。
（林江涛 审校）
J Asthma. 2009 Nov;46(9):884-894.
Effects of tranilast and pentoxifylline in a mouse model of chronic asthma using house dust mite antigen.
Kim SJ, Kim JW, Kim YH, Lee SH, Yoon HK, Kim CH, Ahn JH, Lee JM, Kim JS, Kim SC, Lee SY, Kwon SS, Kim YK.
Tranilast has been used in allergic diseases because of its inhibitory effect on mast cells; it also has an anti-fibrotic effect in several diseases. Pentoxifylline (PTX), a methylxanthine derivative, is a potent anti-inflammatory drug that is known to manifest its effect through the inhibition of Th1 cytokine, but with an uncertain effect on Th2 cytokine. Seven-week-old female BALB/c mice were studied as a chronic asthma model. The mice were challenged with house dust mite (HDM) antigen for 7 weeks. Each group of mice was given an intraperitoneal injection of tranilast, PTX, or tranilast plus PTX before antigen administration. In this mouse model of chronic asthma, tranilast, and PTX each had an inhibitory effect on airway remodeling as well as on airway hyperresponsiveness (AHR) and airway inflammation. The improved events of these drugs were related with the inhibition of the Th2 cytokine IL-13 and TGF-beta 1. Immunohistochemical analysis showed that decreases in the peribronchial trichrome stained area in each treatment group were associated with improvements in the peribronchial smooth muscle hyperplasia, collagen type I, and collagen type III deposition. These drugs could have potential beneficial effects on chronic asthma, especially with respect to airway remodeling.
J Asthma. 2009 Nov;46(9):884-94.
本实验用7周龄雌性BALB/c小鼠建立慢性哮喘模型。小鼠连续7周采用屋尘螨（HDM）抗原致敏。致敏前，每组小鼠腹腔分别注射曲尼司特、PTX或联合注射曲尼司特与PTX。小鼠慢性哮喘模型中，曲尼司特和PTX均能抑制气道重构、减轻气道高反应性（AHR）和气道炎症。上述保护作用与对Th2细胞因子IL-13和TGF-β1抑制相关。
免疫组织化学分析结果显示，各组支气管周围三色染色区域下降与支气管周围平滑肌细胞增生、1型和3型胶原沉积改善相关。上述药物对慢性哮喘，尤其是气道重构方面具有潜在治疗作用。
（林江涛 审校）
J Asthma. 2009 Nov;46(9):884-894.
Effects of tranilast and pentoxifylline in a mouse model of chronic asthma using house dust mite antigen.
Kim SJ, Kim JW, Kim YH, Lee SH, Yoon HK, Kim CH, Ahn JH, Lee JM, Kim JS, Kim SC, Lee SY, Kwon SS, Kim YK.
Tranilast has been used in allergic diseases because of its inhibitory effect on mast cells; it also has an anti-fibrotic effect in several diseases. Pentoxifylline (PTX), a methylxanthine derivative, is a potent anti-inflammatory drug that is known to manifest its effect through the inhibition of Th1 cytokine, but with an uncertain effect on Th2 cytokine. Seven-week-old female BALB/c mice were studied as a chronic asthma model. The mice were challenged with house dust mite (HDM) antigen for 7 weeks. Each group of mice was given an intraperitoneal injection of tranilast, PTX, or tranilast plus PTX before antigen administration. In this mouse model of chronic asthma, tranilast, and PTX each had an inhibitory effect on airway remodeling as well as on airway hyperresponsiveness (AHR) and airway inflammation. The improved events of these drugs were related with the inhibition of the Th2 cytokine IL-13 and TGF-beta 1. Immunohistochemical analysis showed that decreases in the peribronchial trichrome stained area in each treatment group were associated with improvements in the peribronchial smooth muscle hyperplasia, collagen type I, and collagen type III deposition. These drugs could have potential beneficial effects on chronic asthma, especially with respect to airway remodeling.
J Asthma. 2009 Nov;46(9):884-94.
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奥马珠单抗治疗期间FcepsilonRI介导的人嗜碱性粒细胞促哮喘细胞因子和炎症趋化因子释放下降
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