过敏性哮喘患者中的Th17免疫
2009/12/15
背景：过敏性哮喘是一类炎症性疾病，由T辅助细胞（Th）进行调节。Th1/Th2失衡是过敏性哮喘的主要发病机制之一。最近的研究显示Th17细胞参与了动物过敏性哮喘的发生。然而， Th17是否能在过敏性哮喘患者中介导系统性免疫反应尚不清楚。
方法：对29名哮喘患者和12名健康对照者的外周血单核细胞进行分离。采用流式细胞仪对Th1、Th2和Th17细胞进行计数。采用酶联免疫分析法及Luminex法检测血浆和细胞培养液中相关细胞因子（干扰素γ、IL-4, IL-17、IL-22、IL-23和IL-25）的含量。维甲酸相关孤儿受体γt（控制Th17细胞分化的关键转录因子；RORγt）水平采用实时定量多聚酶链反应检测。
结果：与对照组相比，过敏性哮喘患者的Th2和Th17细胞及Th2和Th17相关细胞因子的水平较高，即使在接受过吸入性糖皮质激素治疗的过敏性哮喘患者中也是如此。Th17细胞百分数及血浆IL-17及IL-22含量随着疾病严重程度增加呈现增加趋势，而IL-25水平在轻度哮喘患者中出现上升。过敏性哮喘患者出现IL-17和IL-23含量及RORγt 水平的相应增加。
结论：我们的结果显示，除了Th2细胞免疫，异常的Th17免疫可能也参与了过敏性哮喘的发病过程。
（陈欣 审校）
Int Arch Allergy Immunol. 2009 Oct 21;151(4):297-307. [Epub ahead of print]
Th17 Immunity in Patients with Allergic Asthma.
Zhao Y, Yang J, Gao YD, Guo W.
Department of Respiratory Medicine, Zhongnan Hospital and Department of Respiratory Medicine, Renmin Hospital, Wuhan, China.
Background: Allergic asthma is an inflammatory disease regulated by the T helper (Th) cells. The Th1/Th2 imbalance has been well documented in the pathogenesis of allergic asthma. Recently, Th17 cells have been found to participate in the development of allergic asthma in animals. However, whether Th17 immunity contributes to the systemic immune responses in allergic asthmatic patients is unclear.
Methods: Peripheral blood mononuclear cells were isolated from allergic asthmatics (n = 29) and healthy controls (n = 12). The frequencies of Th1, Th2 and Th17 cells were analyzed by flow cytometry. The related cytokine (IFN-gamma, IL-4, IL-17, IL-22, IL-23 and IL-25) concentrations in plasma and culture supernatants were measured by enzyme-linked immunosorbent assay and Luminex. The level of retinoic acid-related orphan receptor gammat (RORgammat), a key transcription factor controlling Th17 differentiation, was examined by real-time quantitative polymerase chain reaction.
Results: The percentages of Th2 and Th17 cells as well as the concentrations of Th2- and Th17-related cytokines were higher in allergic asthmatics than those in healthy controls; some patients were even treated with inhaled glucocorticoid. The percentages of Th17 cells as well as the plasma concentrations of IL-17 and IL-22 tended to increase with the severity of the disease, while the IL-25 level was elevated in mild patients. A parallel elevation of IL-17 and IL-23 concentrations and an increase in RORgammat level were found in allergic asthmatics.
Conclusion: Our results suggest that besides predominant Th2 immunity, abnormal Th17 immunity may be also involved in the pathogenesis of allergic asthma.
Int Arch Allergy Immunol. 2009 Oct 21;151(4):297-307. [Epub ahead of print]
方法：对29名哮喘患者和12名健康对照者的外周血单核细胞进行分离。采用流式细胞仪对Th1、Th2和Th17细胞进行计数。采用酶联免疫分析法及Luminex法检测血浆和细胞培养液中相关细胞因子（干扰素γ、IL-4, IL-17、IL-22、IL-23和IL-25）的含量。维甲酸相关孤儿受体γt（控制Th17细胞分化的关键转录因子；RORγt）水平采用实时定量多聚酶链反应检测。
结果：与对照组相比，过敏性哮喘患者的Th2和Th17细胞及Th2和Th17相关细胞因子的水平较高，即使在接受过吸入性糖皮质激素治疗的过敏性哮喘患者中也是如此。Th17细胞百分数及血浆IL-17及IL-22含量随着疾病严重程度增加呈现增加趋势，而IL-25水平在轻度哮喘患者中出现上升。过敏性哮喘患者出现IL-17和IL-23含量及RORγt 水平的相应增加。
结论：我们的结果显示，除了Th2细胞免疫，异常的Th17免疫可能也参与了过敏性哮喘的发病过程。
（陈欣 审校）
Int Arch Allergy Immunol. 2009 Oct 21;151(4):297-307. [Epub ahead of print]
Th17 Immunity in Patients with Allergic Asthma.
Zhao Y, Yang J, Gao YD, Guo W.
Department of Respiratory Medicine, Zhongnan Hospital and Department of Respiratory Medicine, Renmin Hospital, Wuhan, China.
Background: Allergic asthma is an inflammatory disease regulated by the T helper (Th) cells. The Th1/Th2 imbalance has been well documented in the pathogenesis of allergic asthma. Recently, Th17 cells have been found to participate in the development of allergic asthma in animals. However, whether Th17 immunity contributes to the systemic immune responses in allergic asthmatic patients is unclear.
Methods: Peripheral blood mononuclear cells were isolated from allergic asthmatics (n = 29) and healthy controls (n = 12). The frequencies of Th1, Th2 and Th17 cells were analyzed by flow cytometry. The related cytokine (IFN-gamma, IL-4, IL-17, IL-22, IL-23 and IL-25) concentrations in plasma and culture supernatants were measured by enzyme-linked immunosorbent assay and Luminex. The level of retinoic acid-related orphan receptor gammat (RORgammat), a key transcription factor controlling Th17 differentiation, was examined by real-time quantitative polymerase chain reaction.
Results: The percentages of Th2 and Th17 cells as well as the concentrations of Th2- and Th17-related cytokines were higher in allergic asthmatics than those in healthy controls; some patients were even treated with inhaled glucocorticoid. The percentages of Th17 cells as well as the plasma concentrations of IL-17 and IL-22 tended to increase with the severity of the disease, while the IL-25 level was elevated in mild patients. A parallel elevation of IL-17 and IL-23 concentrations and an increase in RORgammat level were found in allergic asthmatics.
Conclusion: Our results suggest that besides predominant Th2 immunity, abnormal Th17 immunity may be also involved in the pathogenesis of allergic asthma.
Int Arch Allergy Immunol. 2009 Oct 21;151(4):297-307. [Epub ahead of print]
