白介素-17A在慢性鼻-鼻窦炎伴哮喘相关性鼻息肉患者的嗜酸性粒细胞积聚和粘膜重构中的作用
2009/10/15
背景：白介素（IL）-17A是一种强效炎性因子，对诸多组织的基质细胞都具有较强作用。虽有研究显示IL-17A可通过触发中性粒细胞积聚而参与炎性肺病的发生，但有关其在上呼吸道疾病中的作用尚不清楚。本试验旨在研究慢性鼻-鼻窦炎伴哮喘相关性鼻息肉患者中的IL-17A表达与相关作用。
方法：采用免疫组织化学方法及实时定量RT-PCR检测IL-17A。采用EG2、CD4和中性粒细胞弹性蛋白酶双染色技术检测IL-17A的细胞来源。通过检测上皮损伤程度和基底膜厚度对鼻息肉的组织重构进行评价。
结果：与正常鼻窦粘膜相比，鼻息肉中IL-17A的免疫反应性及其mRNA的表达均显著增加。IL-17A的表达主要定位于嗜酸性粒细胞和CD4阳性淋巴细胞中。而且，IL-17A阳性细胞数量与组织中的嗜酸性粒细胞，而非中性粒细胞相关。上皮损伤程度和基底膜厚度依赖于所浸润的IL-17A阳性细胞数量。
结论：本研究首次发现，在哮喘相关性慢性鼻-鼻窦炎患者中，IL-17A对鼻息肉中嗜酸性粒细胞的聚集和鼻息肉的组织重构起着重要作用。
（苏楠 审校）
Int Arch Allergy Immunol. 2009 Aug 6;151(1):8-16.
Role of Interleukin-17A in the Eosinophil Accumulation and Mucosal Remodeling in Chronic Rhinosinusitis with Nasal Polyps Associated with Asthma.
Saitoh T, Kusunoki T, Yao T, Kawano K, Kojima Y, Miyahara K, Onoda J, Yokoi H, Ikeda K.
Background: Interleukin (IL)-17A is a highly inflammatory cytokine with a robust effect on stromal cells in many tissues. Although IL-17A is known to be associated with inflammatory lung disorders by triggering an accumulation of neutrophils, the effect of IL-17A on the upper airway is still uncertain. The expression of IL-17A and its role were investigated in the nasal polyps of chronic rhinosinusitis associated with asthma.
Methods: IL-17A was detected by immunohistochemistry and quantitative real-time RT-PCR. The cellular source of IL-17A was examined by double staining with EG2, CD4 and neutrophil elastase. The tissue remodeling of the nasal polyps was evaluated by assessing the epithelial damage and basement membrane thickness.
Results: Both the immunoreactivity and mRNA of IL-17A were significantly detected in the nasal polyps in comparison with control normal sinus mucosa. The localization of IL-17A expression predominantly coincided with eosinophils and CD4-positive lymphocytes. Furthermore, the number of IL-17A-positive cells correlated with tissue eosinophils, but not with neutrophils. The degree of epithelial damage and basement membrane thickness was dependent on the number of infiltrated IL-17A-positive cells.
Conclusion: The present study suggests, for the first time, that IL-17A plays an important role in the eosinophil accumulation in the nasal polyps and the remodeling of the nasal polyps of chronic rhinosinusitis associated with asthma.
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吸烟对正常大鼠和卵清蛋白致敏大鼠支气管上皮细胞的改变存在差异
