使用Sauchinone抑制过敏原诱导的、Th2驱动的气道炎症
2009/07/09
Sauchinone是一种从三白草根分离的木脂素复合物。近期体外实验发现，Sauchinone能通过抑制NF-kB p65的活性而表大抗炎活性。
本研究评价sauchinone对小鼠哮喘模型气道炎症的作用。
结果显示，sauchinone 能显著抑制支气管肺泡灌洗液（BAL）中的嗜酸性粒细胞和淋巴细胞浸润，气道粘膜杯状细胞增生和胶原沉积也得到改善。此外，肺内炎症细胞因子IL-13、IL-5和嗜酸性粒细胞趋化因子eotaxin，以及引流淋巴结IL-5在sauchinone处理后显著下降。而且，体外实验还发现，sauchinone能抑制Th2的细胞发育及其IL-4的产生，同时能剂量依赖性的抑制GATA-3介导的IL-5启动子活性。总之，sauchinone能减轻过敏原诱导的气道炎症反应，这可能与抑制Th2细胞发育中的GATA-3活性有关。因此，Sauchinone有可能成为哮喘和过敏性鼻炎的治疗药物。
（陈欣 审校）
Biochem Biophys Res Commun. 2009 May 18. [Epub ahead of print]
Suppression of Th2-driven, allergen-induced airway inflammation by sauchinone.
Min HJ, Won HY, Kim YC, Sung SH, Byun MR, Hwang JH, Hong JH, Hwang ES.
Sauchinone, a lignan compound isolated from the root of Saururus chinensis, has been recently demonstrated to exhibit anti-inflammatory activity via the suppression of NF-kB p65 activity in vitro. In an effort to evaluate the in vivo anti-inflammatory function of sauchinone, we have evaluated the effects of sauchinone on allergen-induced airway inflammation using a murine model of allergic asthma. We observed that marked eosinophilic and lymphocyte infiltration in the BAL fluid were suppressed to a significant degree by sauchinone, and that mucus-secreting goblet cell hyperplasia and collagen deposition in the airways were also ameliorated by administration of sauchinone treatment. Moreover, gene expression of the inflammatory cytokines, IL-13, and IL-5 and eotaxin in the lung, and IL-5 in the draining lymph node were significantly decreased in sauchinone-treated mice. We demonstrated that sauchinone repressed Th2 cell development in vitro and IL-4 production by Th2 cells, and also inhibited GATA-3-mediated IL-5 promoter activity in a dose-dependent manner. Collectively, sauchinone ameliorated allergen-induced airway inflammation, in part, by repressing GATA-3 activity for Th2 cell development, indicating the possible therapeutic potential of sauchinone in airway inflammatory diseases including allergic asthma and rhinitis.
Sauchinone, a lignan compound isolated from the root of Saururus chinensis, has been recently demonstrated to exhibit anti-inflammatory activity via the suppression of NF-kB p65 activity in vitro. In an effort to evaluate the in vivo anti-inflammatory function of sauchinone, we have evaluated the effects of sauchinone on allergen-induced airway inflammation using a murine model of allergic asthma. We observed that marked eosinophilic and lymphocyte infiltration in the BAL fluid were suppressed to a significant degree by sauchinone, and that mucus-secreting goblet cell hyperplasia and collagen deposition in the airways were also ameliorated by administration of sauchinone treatment. Moreover, gene expression of the inflammatory cytokines, IL-13, and IL-5 and eotaxin in the lung, and IL-5 in the draining lymph node were significantly decreased in sauchinone-treated mice. We demonstrated that sauchinone repressed Th2 cell development in vitro and IL-4 production by Th2 cells, and also inhibited GATA-3-mediated IL-5 promoter activity in a dose-dependent manner. Collectively, sauchinone ameliorated allergen-induced airway inflammation, in part, by repressing GATA-3 activity for Th2 cell development, indicating the possible therapeutic potential of sauchinone in airway inflammatory diseases including allergic asthma and rhinitis.
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